The t-SNARE AtVAM3p resides on the prevacuolar compartment in Arabidopsis root cells
Protein cargo is trafficked between the organelles of the endomembrane system inside transport vesicles, a process mediated by integral membrane proteins called SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors) that reside on the surface of the vesicle (v-SNAREs) and target membrane (t-SNAREs). In examining transport of cargo between the trans-Golgi network and the vacuole in Arabidopsis, the authors have previously characterized AtPEP12p as a t-SNARE residing on the prevacuolar compartment and AtVTl1a as a v-SNARE that interacts with AtPEP12p. Recently, they have begun to characterize AtVAM3p, another Arabidopsis t-SNARE that shows high sequence homology to AtPEP12p. They have found that AtVTl1a also interacts with AtVAM3p, suggesting a role for this t-SNARE in post-Golgi trafficking. AtVAM3p has been suggested to localize to the vacuolar membrane in Arabidopsis cells; however, using specific antisera and expression of epitope-tagged versions of each t-SNARE, the authors have discovered that AtVAM3p is found on the same prevacuolar structure as AtPEP12p in Arabidopsis root cells.
- Research Organization:
- Michigan State Univ., East Lansing, MI (US)
- OSTI ID:
- 20006202
- Journal Information:
- Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 3 Vol. 121; ISSN 0032-0889; ISSN PLPHAY
- Country of Publication:
- United States
- Language:
- English