Enhanced biotransformation of carbon tetrachloride by Acetobacterium woodii upon addition of hydroxocobalamin and fructose
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:20001034
The objective of this study was to evaluate the effect of hydroxocobalamin (OH-Cbl) on transformation of high concentrations of carbon tetrachloride (CT) by Acetobacterium woodii. Complete transformation of 470 {micro}M CT was achieved by A. woodii within 2.5 days, when 10 {micro}M OH-Cbl was added along with 25.2 mM fructose. This was approximately 30 times faster than A. woodii cultures and medium that did not receive OH-Cbl and 5 times faster than those controls that did receive OH-Cbl, but either live A. woodii or fructose was missing. CT transformation in treatments with only OH-Cbl was indicative of the important contribution of nonenzymatic reactions. Besides increasing the rate of CT transformation, addition of fructose and OH-Cbl to live cultures increased the percentage of [{sup 14}C]CT transformed to {sup 14}CO{sub 2} and {sup 14}C-labeled soluble materials, while decreasing the percentage of CT reduced to chloroform and abiotically transformed to carbon disulfide. {sup 14}CS{sub 2} represented more than 35% of the [{sup 14}C]CT in the presence of reduced medium and OH-Cbl. Conversion of CT to CO was a predominant pathway in formation of CO{sub 2} in the presence of live cells and added fructose and OH-Cbl. These results indicate that the rate and distribution of products during cometabolic transformation of CT by A. woodii can be improved by the addition of fructose and OH-Cbl.
- Research Organization:
- Michigan State Univ., East Lansing, MI (US)
- Sponsoring Organization:
- Oak Ridge Institute for Science and Education; US Department of Energy
- OSTI ID:
- 20001034
- Journal Information:
- Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 10 Vol. 65; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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