skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microbial reductive dechlorination in large-scale sandbox model

Abstract

Microbial reductive dechlorination is a naturally occurring transformation process tetrachloroethene (PCE) undergoes in aquifers. It contributes significantly to natural attenuation of chlorinated solvents. Stimulation of this process has been considered as a method of enhanced bioremediation. Experiments on the stimulation of reductive dechlorination were carried out in a large-scale quasi-2D sandbox model. The transformation of PCE to cis-1,2-dichloroethene (DCE) was attempted by inoculation with Dehalospirillum multivorans and that of DCE to ethene with a mixed culture. Ethanol used as the electron donor was introduced into the inlet of the domain, whereas water loaded with PCE was injected into a well. Limitations due to insufficient mixing could not be observed as high-permeability lenses enhanced the transverse exchange of the compounds. Both reductive dechlorination and competitive microbial reactions led to the acidification of the domain. The artificial aquifer was buffered by the concurrent injection of sodium sulfide with the electron donor. Under these conditions bioaugmentation of Dehalospirillum multivorans was successful, whereas stable dechlorination of DCE could not be achieved.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Univ. Stuttgart (DE)
OSTI Identifier:
20000237
Resource Type:
Journal Article
Journal Name:
Journal of Environmental Engineering (New York)
Additional Journal Information:
Journal Volume: 125; Journal Issue: 9; Other Information: PBD: Sep 1999; Journal ID: ISSN 0733-9372
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; CHLORINATED ALIPHATIC HYDROCARBONS; REMEDIAL ACTION; SCALE MODELS; BIODEGRADATION; DECHLORINATION; AQUIFERS

Citation Formats

Cirpka, O.A., Windfuhr, C., Bisch, G., Granzow, S., Scholz-Muramatsu, H., and Kobus, H. Microbial reductive dechlorination in large-scale sandbox model. United States: N. p., 1999. Web. doi:10.1061/(ASCE)0733-9372(1999)125:9(861).
Cirpka, O.A., Windfuhr, C., Bisch, G., Granzow, S., Scholz-Muramatsu, H., & Kobus, H. Microbial reductive dechlorination in large-scale sandbox model. United States. doi:10.1061/(ASCE)0733-9372(1999)125:9(861).
Cirpka, O.A., Windfuhr, C., Bisch, G., Granzow, S., Scholz-Muramatsu, H., and Kobus, H. Wed . "Microbial reductive dechlorination in large-scale sandbox model". United States. doi:10.1061/(ASCE)0733-9372(1999)125:9(861).
@article{osti_20000237,
title = {Microbial reductive dechlorination in large-scale sandbox model},
author = {Cirpka, O.A. and Windfuhr, C. and Bisch, G. and Granzow, S. and Scholz-Muramatsu, H. and Kobus, H.},
abstractNote = {Microbial reductive dechlorination is a naturally occurring transformation process tetrachloroethene (PCE) undergoes in aquifers. It contributes significantly to natural attenuation of chlorinated solvents. Stimulation of this process has been considered as a method of enhanced bioremediation. Experiments on the stimulation of reductive dechlorination were carried out in a large-scale quasi-2D sandbox model. The transformation of PCE to cis-1,2-dichloroethene (DCE) was attempted by inoculation with Dehalospirillum multivorans and that of DCE to ethene with a mixed culture. Ethanol used as the electron donor was introduced into the inlet of the domain, whereas water loaded with PCE was injected into a well. Limitations due to insufficient mixing could not be observed as high-permeability lenses enhanced the transverse exchange of the compounds. Both reductive dechlorination and competitive microbial reactions led to the acidification of the domain. The artificial aquifer was buffered by the concurrent injection of sodium sulfide with the electron donor. Under these conditions bioaugmentation of Dehalospirillum multivorans was successful, whereas stable dechlorination of DCE could not be achieved.},
doi = {10.1061/(ASCE)0733-9372(1999)125:9(861)},
journal = {Journal of Environmental Engineering (New York)},
issn = {0733-9372},
number = 9,
volume = 125,
place = {United States},
year = {1999},
month = {9}
}