Complex regulation in a Comamonas platform for diverse aromatic carbon metabolism

Wilkes, Rebecca A.; Waldbauer, Jacob; Caroll, Austin; Nieto-Domínguez, Manuel; Parker, Darren J.; Zhang, Lichun; Guss, Adam M.; Aristilde, Ludmilla

doi:10.1038/s41589-022-01237-7

Complex regulation in a Comamonas platform for diverse aromatic carbon metabolism

Journal Article · Mon Feb 06 00:00:00 EST 2023 · Nature Chemical Biology

DOI:https://doi.org/10.1038/s41589-022-01237-7· OSTI ID:1997683

^[1]; Waldbauer, Jacob ^[2]; Caroll, Austin ^[3]; ^[4]; Parker, Darren J. ^[3]; Zhang, Lichun ^[2]; ^[3]; ^[1]

Cornell University, Ithaca, NY (United States); Northwestern University, Evanston, IL (United States)
University of Chicago, IL (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Technical University of Denmark, Lyngby (Denmark)

Critical to a sustainable energy future are microbial platforms that can process aromatic carbons from the largely untapped reservoir of lignin and plastic feedstocks. Comamonas species present promising bacterial candidates for such platforms because they can use a range of natural and xenobiotic aromatic compounds and often possess innate genetic constraints that avoid competition with sugars. However, the metabolic reactions of these species are underexplored, and the regulatory mechanisms are unknown. Here we identify multilevel regulation in the conversion of lignin-related natural aromatic compounds, 4-hydroxybenzoate and vanillate, and the plastics-related xenobiotic aromatic compound, terephthalate, in Comamonas testosteroni KF-1. Transcription-level regulation controls initial catabolism and cleavage, but metabolite-level thermodynamic regulation governs fluxes in central carbon metabolism. Quantitative ¹³C mapping of tricarboxylic acid cycle and cataplerotic reactions elucidates key carbon routing not evident from enzyme abundance changes. This scheme of transcriptional activation coupled with metabolic fine-tuning challenges outcome predictions during metabolic manipulations.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1997683

Journal Information:: Nature Chemical Biology, Journal Name: Nature Chemical Biology Journal Issue: 5 Vol. 19; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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