Production of Designer Xylose-Acetic Acid Enriched Hydrolysate from Bioenergy Sorghum, Oilcane, and Energycane Bagasses

Cheng, Ming-Hsun; Singh, Shuchi; Carr Clennon, Aidan N.; Dien, Bruce S.; Singh, Vijay

doi:10.1016/j.biortech.2023.129104

Production of Designer Xylose-Acetic Acid Enriched Hydrolysate from Bioenergy Sorghum, Oilcane, and Energycane Bagasses

Journal Article · Fri Apr 28 04:00:00 EDT 2023 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2023.129104· OSTI ID:1996367

Cheng, Ming-Hsun ^[1]; ^[2]; Carr Clennon, Aidan N. ^[2]; Dien, Bruce S. ^[3]; ^[2]

University of Illinois Urbana-Champaign, IL (United States); University of Idaho, Idaho Falls, ID (United States); UIUC
University of Illinois Urbana-Champaign, IL (United States)
University of Illinois Urbana-Champaign, IL (United States); United States Department of Agriculture (USDA), Peoria, IL (United States)

Xylan accounts for up to 40% of the structural carbohydrates in lignocellulosic feedstocks. Along with xylan, acetic acid in sources of hemicellulose can be recovered and marketed as a commodity chemical. Through vibrant bioprocessing innovations, converting xylose and acetic acid into high-value bioproducts via microbial cultures improves the feasibility of lignocellulosic biorefineries. Enzymatic hydrolysis using xylanase supplemented with acetylxylan esterase (AXE) was applied to prepare xylose-acetic acid enriched hydrolysates from bioenergy sorghum, oilcane, or energycane using sequential hydrothermal-mechanical pretreatment. Various biomass solids contents (15 to 25%, w/v) and xylanase loadings (140 to 280 FXU/g biomass) were tested to maximize xylose and acetic acid titers. The xylose and acetic acid yields were significantly improved by supplementing with AXE. The optimal yields of xylose and acetic acid were 92.29% and 62.26% obtained from hydrolyzing energycane and oilcane at 25% and 15% w/v biomass solids using 280 FXU xylanase/g biomass and AXE, respectively.

View Accepted Manuscript (DOE)

Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 1996367

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 380; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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