Tectonic and geological setting influence hot spring microbiology

Colman, Daniel R.; Veach, Allison; Stefánsson, Andri; Wurch, Louie; Belisle, B. Shafer; Podar, Peter T.; Yang, Zamin; Klingeman, Dawn Marie; Senba, Kazuyo; Murakami, Katsuhiko S.; Kristjánsson, Jakob K.; Björnsdóttir, Snædís H.; Boyd, Eric S.; Podar, Mircea

doi:10.1111/1462-2920.16472

Tectonic and geological setting influence hot spring microbiology

Journal Article · Tue Aug 08 00:00:00 EDT 2023 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.16472· OSTI ID:1994676

^[1]; Veach, Allison ^[2]; Stefánsson, Andri ^[3]; Wurch, Louie ^[4]; Belisle, B. Shafer ^[5]; Podar, Peter T. ^[6]; Yang, Zamin ^[5]; ^[5]; Senba, Kazuyo ^[7]; Murakami, Katsuhiko S. ^[8]; Kristjánsson, Jakob K. ^[9]; Björnsdóttir, Snædís H. ^[3]; ^[1]; ^[5]

Montana State Univ., Bozeman, MT (United States)
Univ. of Texas at San Antonio, TX (United States)
University of Iceland (Iceland)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); James Madison Univ., Harrisonburg, VA (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Vanderbilt Univ., Nashville, TN (United States)
Beppu University (Japan)
Pennsylvania State Univ., University Park, PA (United States)
Hripa ehf (Iceland)

Hydrothermal systems form at divergent and convergent boundaries of lithospheric plates and within plates due to weakened crust and mantle plumes, playing host to diverse microbial ecosystems. Little is known of how differences in tectonic setting influence the geochemical and microbial compositions of these hydrothermal ecosystems. Here, for this work, coordinated geochemical and microbial community analyses were conducted on 87 high-temperature (>65°C) water and sediment samples from hot springs in Yellowstone National Park, Wyoming, USA (n = 41; mantle plume setting), Iceland (n = 41, divergent boundary), and Japan (n = 5; convergent boundary). Region-specific variation in geochemistry and sediment-associated 16S rRNA gene amplicon sequence variant (ASV) composition was observed, with 16S rRNA gene assemblages being nearly completely distinguished by region and pH being the most explanatory parameter within regions. Several low abundance ASVs exhibited cosmopolitan distributions across regions, while most high-abundance ASVs were only identified in specific regions. The presence of some cosmopolitan ASVs across regions argues against dispersal limitation primarily shaping the distribution of taxa among regions. Rather, the results point to local tectonic and geologic characteristics shaping the geochemistry of continental hydrothermal systems that then select for distinct microbial assemblages. These results provide new insights into the co-evolution of hydrothermal systems and their microbial communities.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Aeronautics and Space Administration (NASA); National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; SC0006654

OSTI ID:: 1994676

Alternate ID(s):: OSTI ID: 1995558

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 11 Vol. 25; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

References (86)

Pyrobaculum gen. nov., a new genus of neutrophilic, rod-shaped archaebacteria from continental solfataras growing optimally at 100�C Huber, R.; Kristjansson, J. K.; Stetter, K. O. Archives of Microbiology, Vol. 149, Issue 2 https://doi.org/10.1007/BF00425072	journal	December 1987
Rhyolites at Kerlingarfjöll, Iceland: the evolution and lifespan of silicic central volcanoes Flude, Stephanie; McGarvie, David W.; Burgess, Ray Bulletin of Volcanology, Vol. 72, Issue 5 https://doi.org/10.1007/s00445-010-0344-0	journal	February 2010
A review of the mechanisms of mineral-based metabolism in early Earth analog rock-hosted hydrothermal ecosystems Amenabar, Maximiliano J.; Boyd, Eric S. World Journal of Microbiology and Biotechnology, Vol. 35, Issue 2 https://doi.org/10.1007/s11274-019-2604-2	journal	January 2019
Fluid flow processes in the BEPPU geothermal system, Japan Allis, R. G.; Yusa, Y. Geothermics, Vol. 18, Issue 5-6 https://doi.org/10.1016/0375-6505(89)90104-1	journal	January 1989
Geothermal systems in Iceland: Structure and conceptual models—I. High-temperature areas Arnórsson, Stefán Geothermics, Vol. 24, Issue 5-6 https://doi.org/10.1016/0375-6505(95)00025-9	journal	October 1995
Hydrogeochemistry of the Campania region in southern Italy Duchi, Vittorio; Minissale, Angelo; Vaselli, Orlando Journal of Volcanology and Geothermal Research, Vol. 67, Issue 4 https://doi.org/10.1016/0377-0273(94)00109-T	journal	September 1995
Sulfur geochemistry of hydrothermal waters in Yellowstone National Park: IV Acid–sulfate waters Kirk Nordstrom, D.; Blaine McCleskey, R.; Ball, James W. Applied Geochemistry, Vol. 24, Issue 2 https://doi.org/10.1016/j.apgeochem.2008.11.019	journal	February 2009
The fluid geochemistry of Icelandic high temperature geothermal areas Ármannsson, Halldór Applied Geochemistry, Vol. 66 https://doi.org/10.1016/j.apgeochem.2015.10.008	journal	March 2016
The transition to microbial photosynthesis in hot spring ecosystems Cox, Alysia; Shock, Everett L.; Havig, Jeff R. Chemical Geology, Vol. 280, Issue 3-4 https://doi.org/10.1016/j.chemgeo.2010.11.022	journal	January 2011
The chemistry of trace elements in surface geothermal waters and steam, Iceland Kaasalainen, Hanna; Stefánsson, Andri Chemical Geology, Vol. 330-331 https://doi.org/10.1016/j.chemgeo.2012.08.019	journal	November 2012
Geochemistry of thermal waters in the Southern Volcanic Zone, Chile – Implications for structural controls on geothermal fluid composition Wrage, Jackie; Tardani, Daniele; Reich, Martin Chemical Geology, Vol. 466 https://doi.org/10.1016/j.chemgeo.2017.07.004	journal	September 2017
Geochemistry of volcanic and hydrothermal fluids and volatile budget of the Kamchatka–Kuril subduction zone Taran, Yuri A. Geochimica et Cosmochimica Acta, Vol. 73, Issue 4 https://doi.org/10.1016/j.gca.2008.11.020	journal	February 2009
Quantifying inorganic sources of geochemical energy in hydrothermal ecosystems, Yellowstone National Park, USA Shock, Everett L.; Holland, Melanie; Meyer-Dombard, D’Arcy Geochimica et Cosmochimica Acta, Vol. 74, Issue 14 https://doi.org/10.1016/j.gca.2009.08.036	journal	July 2010
Geochemistry of hot springs in the Tengchong hydrothermal areas, Southwestern China Guo, Qinghai; Wang, Yanxin Journal of Volcanology and Geothermal Research, Vol. 215-216 https://doi.org/10.1016/j.jvolgeores.2011.12.003	journal	February 2012
Origins of geothermal gases at Yellowstone Lowenstern, Jacob B.; Bergfeld, Deborah; Evans, William C. Journal of Volcanology and Geothermal Research, Vol. 302 https://doi.org/10.1016/j.jvolgeores.2015.06.010	journal	September 2015
The source, fate, and transport of arsenic in the Yellowstone hydrothermal system - An overview McCleskey, R. Blaine; Nordstrom, D. Kirk; Hurwitz, Shaul Journal of Volcanology and Geothermal Research, Vol. 432 https://doi.org/10.1016/j.jvolgeores.2022.107709	journal	December 2022
Uncultivated thermophiles: current status and spotlight on ‘Aigarchaeota’ Hedlund, Brian P.; Murugapiran, Senthil K.; Alba, Timothy W. Current Opinion in Microbiology, Vol. 25 https://doi.org/10.1016/j.mib.2015.06.008	journal	June 2015
Geological setting of Earth's oldest fossils in the ca. 3.5Ga Dresser Formation, Pilbara Craton, Western Australia Vankranendonk, M.; Philippot, P.; Lepot, K. Precambrian Research, Vol. 167, Issue 1-2 https://doi.org/10.1016/j.precamres.2008.07.003	journal	November 2008
Fluoride in thermal and non-thermal groundwater: Insights from geochemical modeling Nordstrom, D. Kirk Science of The Total Environment, Vol. 824 https://doi.org/10.1016/j.scitotenv.2022.153606	journal	June 2022
Relative Abundance of Thiolated Species of As, Mo, W, and Sb in Hot Springs of Yellowstone National Park and Iceland Planer-Friedrich, Britta; Forberg, Judith; Lohmayer, Regina Environmental Science & Technology, Vol. 54, Issue 7 https://doi.org/10.1021/acs.est.0c00668	journal	March 2020
Micro-organisms adapted to High Temperatures Brock, Thomas D. Nature, Vol. 214, Issue 5091 https://doi.org/10.1038/214882a0	journal	May 1967
[FeFe]-hydrogenase in Yellowstone National Park: evidence for dispersal limitation and phylogenetic niche conservatism Boyd, Eric S.; Hamilton, Trinity L.; Spear, John R. The ISME Journal, Vol. 4, Issue 12 https://doi.org/10.1038/ismej.2010.76	journal	June 2010
Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park Kozubal, Mark A.; Romine, Margaret; Jennings, Ryan deM The ISME Journal, Vol. 7, Issue 3 https://doi.org/10.1038/ismej.2012.132	journal	November 2012
Humboldt’s spa: microbial diversity is controlled by temperature in geothermal environments Sharp, Christine E.; Brady, Allyson L.; Sharp, Glen H. The ISME Journal, Vol. 8, Issue 6 https://doi.org/10.1038/ismej.2013.237	journal	January 2014
Geobiological feedbacks and the evolution of thermoacidophiles Colman, Daniel R.; Poudel, Saroj; Hamilton, Trinity L. The ISME Journal, Vol. 12, Issue 1 https://doi.org/10.1038/ismej.2017.162	journal	October 2017
Earliest signs of life on land preserved in ca. 3.5 Ga hot spring deposits Djokic, Tara; Van Kranendonk, Martin J.; Campbell, Kathleen A. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15263	journal	May 2017
Practical innovations for high-throughput amplicon sequencing Lundberg, Derek S.; Yourstone, Scott; Mieczkowski, Piotr Nature Methods, Vol. 10, Issue 10 https://doi.org/10.1038/nmeth.2634	journal	September 2013
QIIME allows analysis of high-throughput community sequencing data Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse Nature Methods, Vol. 7, Issue 5 https://doi.org/10.1038/nmeth.f.303	journal	April 2010
Chemolithoautotroph distributions across the subsurface of a convergent margin Rogers, Timothy J.; Buongiorno, Joy; Jessen, Gerdhard L. The ISME Journal, Vol. 17, Issue 1 https://doi.org/10.1038/s41396-022-01331-7	journal	October 2022
Microbial biogeography of 925 geothermal springs in New Zealand Power, Jean F.; Carere, Carlo R.; Lee, Charles K. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05020-y	journal	July 2018
Mixing of meteoric and geothermal fluids supports hyperdiverse chemosynthetic hydrothermal communities Colman, Daniel R.; Lindsay, Melody R.; Boyd, Eric S. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-08499-1	journal	February 2019
An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea Buessecker, Steffen; Palmer, Marike; Lai, Dengxun Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-31452-8	journal	June 2022
Effect of tectonic processes on biosphere–geosphere feedbacks across a convergent margin Fullerton, Katherine M.; Schrenk, Matthew O.; Yücel, Mustafa Nature Geoscience, Vol. 14, Issue 5 https://doi.org/10.1038/s41561-021-00725-0	journal	April 2021
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 Bolyen, Evan; Rideout, Jai Ram; Dillon, Matthew R. Nature Biotechnology, Vol. 37, Issue 8 https://doi.org/10.1038/s41587-019-0209-9	journal	July 2019
Subsurface Archaea associated with rapid geobiological change in a model Yellowstone hot spring Colman, Daniel R.; Amenabar, Maximiliano J.; Fernandes-Martins, Maria C. Communications Earth & Environment, Vol. 3, Issue 1 https://doi.org/10.1038/s43247-022-00542-2	journal	September 2022
Tectonic settings influence the geochemical and microbial diversity of Peru hot springs Upin, Heather E.; Newell, Dennis L.; Colman, Daniel R. Communications Earth & Environment, Vol. 4, Issue 1 https://doi.org/10.1038/s43247-023-00787-5	journal	April 2023
Geographical isolation in hot spring cyanobacteria: Geographical isolation in hot spring cyanobacteria Papke, R. Thane; Ramsing, Niels B.; Bateson, Mary M. Environmental Microbiology, Vol. 5, Issue 8 https://doi.org/10.1046/j.1462-2920.2003.00460.x	journal	July 2003
Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment. Barns, S. M.; Fundyga, R. E.; Jeffries, M. W. Proceedings of the National Academy of Sciences, Vol. 91, Issue 5 https://doi.org/10.1073/pnas.91.5.1609	journal	March 1994
Formate as an Energy Source for Microbial Metabolism in Chemosynthetic Zones of Hydrothermal Ecosystems Windman, Todd; Zolotova, Natalya; Schwandner, Florian Astrobiology, Vol. 7, Issue 6 https://doi.org/10.1089/ast.2007.0127	journal	December 2007
The Intersection of Geology, Geochemistry, and Microbiology in Continental Hydrothermal Systems Colman, Daniel R.; Lindsay, Melody R.; Amenabar, Maximiliano J. Astrobiology, Vol. 19, Issue 12 https://doi.org/10.1089/ast.2018.2016	journal	December 2019
To rarefy or not to rarefy: robustness and efficiency trade-offs of rarefying microbiome data Hong, Johnny; Karaoz, Ulas; de Valpine, Perry Bioinformatics, Vol. 38, Issue 9 https://doi.org/10.1093/bioinformatics/btac127	journal	February 2022
Cytoscape 2.8: new features for data integration and network visualization Smoot, M. E.; Ono, K.; Ruscheinski, J. Bioinformatics, Vol. 27, Issue 3 https://doi.org/10.1093/bioinformatics/btq675	journal	December 2010
The biology of thermoacidophilic archaea from the order Sulfolobales Lewis, April M.; Recalde, Alejandra; Bräsen, Christopher FEMS Microbiology Reviews, Vol. 45, Issue 4 https://doi.org/10.1093/femsre/fuaa063	journal	January 2021
Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group Nunoura, Takuro; Takaki, Yoshihiro; Kakuta, Jungo Nucleic Acids Research, Vol. 39, Issue 8 https://doi.org/10.1093/nar/gkq1228	journal	December 2010
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
Petrogenetic Evolution of the Torfaj kull Volcanic Complex, Iceland I. Relationship Between the Magma Types Macdonald, R.; McGARVIE, D. W.; Pinkerton, H. Journal of Petrology, Vol. 31, Issue 2 https://doi.org/10.1093/petrology/31.2.429	journal	April 1990
Conexivisphaera calida gen. nov., sp. nov., a thermophilic sulfur- and iron-reducing archaeon, and proposal of Conexivisphaeraceae fam. nov., Conexivisphaerales ord. nov., and Conexivisphaeria class. nov. in the phylum Thaumarchaeota Kato, Shingo; Ohnishi, Masafumi; Nagamori, Mai International Journal of Systematic and Evolutionary Microbiology, Vol. 71, Issue 1 https://doi.org/10.1099/ijsem.0.004595	journal	January 2021
Distribution of ether lipids and composition of the archaeal community in terrestrial geothermal springs: impact of environmental variables Xie, Wei; Zhang, Chuanlun L.; Wang, Jinxiang Environmental Microbiology, Vol. 17, Issue 5 https://doi.org/10.1111/1462-2920.12595	journal	October 2014
The distribution, diversity and function of predominant Thermoproteales in high-temperature environments of Yellowstone National Park: Diversity of Thermoproteales Jay, Zackary J.; Beam, Jacob P.; Kozubal, Mark A. Environmental Microbiology, Vol. 18, Issue 12 https://doi.org/10.1111/1462-2920.13366	journal	May 2016
Probing the geological source and biological fate of hydrogen in Yellowstone hot springs Lindsay, Melody R.; Colman, Daniel R.; Amenabar, Maximiliano J. Environmental Microbiology, Vol. 21, Issue 10 https://doi.org/10.1111/1462-2920.14730	journal	July 2019
Geologic legacy spanning >90 years explains unique Yellowstone hot spring geochemistry and biodiversity Payne, Devon; Dunham, Eric C.; Mohr, Elizabeth Environmental Microbiology, Vol. 21, Issue 11 https://doi.org/10.1111/1462-2920.14775	journal	August 2019
Relationships between fluid mixing, biodiversity, and chemosynthetic primary productivity in Yellowstone hot springs Fernandes‐Martins, Maria C.; Colman, Daniel R.; Boyd, Eric S. Environmental Microbiology, Vol. 25, Issue 5 https://doi.org/10.1111/1462-2920.16340	journal	January 2023
Chemolithotrophic growth of the aerobic hyperthermophilic bacterium Thermocrinis ruber OC 14/7/2 on monothioarsenate and arsenite Härtig, Cornelia; Lohmayer, Regina; Kolb, Steffen FEMS Microbiology Ecology, Vol. 90, Issue 3 https://doi.org/10.1111/1574-6941.12431	journal	October 2014
Volcanic controls on the microbial habitability of Mars‐analogue hydrothermal environments Moreras‐Marti, Arola; Fox‐Powell, Mark; Zerkle, Aubrey L. Geobiology, Vol. 19, Issue 5 https://doi.org/10.1111/gbi.12459	journal	June 2021
Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes Eiler, Alexander; Bertilsson, Stefan Environmental Microbiology, Vol. 6, Issue 12 https://doi.org/10.1111/j.1462-2920.2004.00657.x	journal	December 2004
Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol de la Torre, José R.; Walker, Christopher B.; Ingalls, Anitra E. Environmental Microbiology, Vol. 10, Issue 3 https://doi.org/10.1111/j.1462-2920.2007.01506.x	journal	March 2008
The Thermophilic Cyanophytes of Iceland and the Upper Temperature Limit Castenholz, Richard W. Journal of Phycology, Vol. 5, Issue 4 https://doi.org/10.1111/j.1529-8817.1969.tb02626.x	journal	December 1969
THE EFFECT OF SULFIDE ON THE BLUEGREEN ALGAE OF HOT SPRINGS. I. NEW ZEALAND AND ICELAND¹ Castenholz, Richard W. Journal of Phycology, Vol. 12, Issue 1 https://doi.org/10.1111/j.1529-8817.1976.tb02826.x	journal	March 1976
Geographic Barriers Isolate Endemic Populations of Hyperthermophilic Archaea Whitaker, R. J. Science, Vol. 301, Issue 5635 https://doi.org/10.1126/science.1086909	journal	August 2003
Bacterial Origin of Sulfuric Acid in Geothermal Habitats Mosser, Jerry L.; Mosser, Anne G.; Brock, Thomas D. Science, Vol. 179, Issue 4080 https://doi.org/10.1126/science.179.4080.1323	journal	March 1973
The Yellowstone magmatic system from the mantle plume to the upper crust Huang, H. -H.; Lin, F. -C.; Schmandt, B. Science, Vol. 348, Issue 6236 https://doi.org/10.1126/science.aaa5648	journal	April 2015
Carbon Source Preference in Chemosynthetic Hot Spring Communities Urschel, Matthew R.; Kubo, Michael D.; Hoehler, Tori M. Applied and Environmental Microbiology, Vol. 81, Issue 11 https://doi.org/10.1128/AEM.00511-15	journal	March 2015
Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park Jay, Z. J.; Beam, J. P.; Dohnalkova, A. Applied and Environmental Microbiology, Vol. 81, Issue 17 https://doi.org/10.1128/AEM.01095-15	journal	June 2015
Bar-Coded Pyrosequencing Reveals Shared Bacterial Community Properties along the Temperature Gradients of Two Alkaline Hot Springs in Yellowstone National Park Miller, S. R.; Strong, A. L.; Jones, K. L. Applied and Environmental Microbiology, Vol. 75, Issue 13 https://doi.org/10.1128/AEM.02792-08	journal	May 2009
Complete and Draft Genome Sequences of Six Members of the Aquificales Reysenbach, A. -L.; Hamamura, N.; Podar, M. Journal of Bacteriology, Vol. 191, Issue 6 https://doi.org/10.1128/JB.01645-08	journal	January 2009
Novel Division Level Bacterial Diversity in a Yellowstone Hot Spring Hugenholtz, Philip; Pitulle, Christian; Hershberger, Karen L. Journal of Bacteriology, Vol. 180, Issue 2 https://doi.org/10.1128/JB.180.2.366-376.1998	journal	January 1998
Thermophilic blue-green algae and the thermal environment Castenholz, R. W. Bacteriological Reviews, Vol. 33, Issue 4 https://doi.org/10.1128/br.33.4.476-504.1969	journal	December 1969
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys Walters, William; Hyde, Embriette R.; Berg-Lyons, Donna mSystems, Vol. 1, Issue 1 https://doi.org/10.1128/mSystems.00009-15	journal	December 2015
Deblur Rapidly Resolves Single-Nucleotide Community Sequence Patterns Amir, Amnon; McDonald, Daniel; Navas-Molina, Jose A. mSystems, Vol. 2, Issue 2 https://doi.org/10.1128/mSystems.00191-16	journal	March 2017
Rate of Sulfuric Acid Formation in Yellowstone National Park Schoen, Robert Geological Society of America Bulletin, Vol. 80, Issue 4 https://doi.org/10.1130/0016-7606(1969)80[643:ROSAFI]2.0.CO;2	journal	January 1969
Bimodal Distribution of pH Values of Thermal Springs of the World Brock, Thomas D. Geological Society of America Bulletin, Vol. 82, Issue 5 https://doi.org/10.1130/0016-7606(1971)82[1393:BDOPVO]2.0.CO;2	journal	January 1971
Geochemistry and Dynamics of the Yellowstone National Park Hydrothermal System Fournier, R. O. Annual Review of Earth and Planetary Sciences, Vol. 17, Issue 1 https://doi.org/10.1146/annurev.ea.17.050189.000305	journal	May 1989
Waste Not, Want Not: Why Rarefying Microbiome Data Is Inadmissible McMurdie, Paul J.; Holmes, Susan PLoS Computational Biology, Vol. 10, Issue 4 https://doi.org/10.1371/journal.pcbi.1003531	journal	April 2014
A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing Hou, Weiguo; Wang, Shang; Dong, Hailiang PLoS ONE, Vol. 8, Issue 1 https://doi.org/10.1371/journal.pone.0053350	journal	January 2013
Cutadapt removes adapter sequences from high-throughput sequencing reads Martin, Marcel EMBnet.journal, Vol. 17, Issue 1 https://doi.org/10.14806/ej.17.1.200	journal	May 2011
Hydrologic, Magmatic, and Tectonic Controls on Hydrothermal Flow, Taupo Volcanic Zone, New Zealand: Implications for the Formation of Epithermal Vein Deposits Rowland, J. V.; Simmons, S. F. Economic Geology, Vol. 107, Issue 3 https://doi.org/10.2113/econgeo.107.3.427	journal	April 2012
Fluid-Fluid Interactions in Geothermal Systems Arnorsson, S.; Stefansson, A.; Bjarnason, J. O. Reviews in Mineralogy and Geochemistry, Vol. 65, Issue 1 https://doi.org/10.2138/rmg.2007.65.9	journal	July 2007
Water-chemistry data for selected springs, geysers, and streams in Yellowstone National Park, Wyoming, 1999-2000 Ball, James W.; McCleskey, R. Blaine; Nordstrom, D. Kirk https://doi.org/10.3133/ofr02382	report	January 2002
The Quaternary and Pliocene Yellowstone Plateau volcanic field of Wyoming, Idaho, and Montana Christiansen, Robert L. USGS Numbered Series https://doi.org/10.3133/pp729G	report	January 2001
Modeling the Habitat Range of Phototrophs in Yellowstone National Park: Toward the Development of a Comprehensive Fitness Landscape Boyd, Eric S.; Fecteau, Kristopher M.; Havig, Jeff R. Frontiers in Microbiology, Vol. 3 https://doi.org/10.3389/fmicb.2012.00221	journal	January 2012
The YNP metagenome project: environmental parameters responsible for microbial distribution in the Yellowstone geothermal ecosystem Inskeep, William Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00067	journal	January 2013
Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China Hedlund, Brian P.; Reysenbach, Anna-Louise; Huang, Liuquin Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00157	journal	February 2015
Cultivation and Genomic Analysis of “Candidatus Nitrosocaldus islandicus,” an Obligately Thermophilic, Ammonia-Oxidizing Thaumarchaeon from a Hot Spring Biofilm in Graendalur Valley, Iceland Daebeler, Anne; Herbold, Craig W.; Vierheilig, Julia Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.00193	journal	February 2018
Comparative Analysis of Microbial Diversity Across Temperature Gradients in Hot Springs From Yellowstone and Iceland Podar, Peter T.; Yang, Zamin; Björnsdóttir, Snædís H. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.01625	journal	July 2020
Ecological and Evolutionary Implications of Microbial Dispersal Custer, Gordon F.; Bresciani, Luana; Dini-Andreote, Francisco Frontiers in Microbiology, Vol. 13 https://doi.org/10.3389/fmicb.2022.855859	journal	April 2022
VSEARCH: a versatile open source tool for metagenomics Rognes, Torbjørn; Flouri, Tomáš; Nichols, Ben PeerJ, Vol. 4 https://doi.org/10.7717/peerj.2584	journal	January 2016