Detection and confirmation of N-nitrosodialkylamines using liquid chromatography-electrospray ionization coupled on-line with a photolysis reactor
- National Center for Toxicological Research, Jefferson, AK (United States)
Simultaneous detection and confirmation of several N-nitrosodialkylamines are accomplished by on-line coupling of a photolysis reactor with an HPLC-electrospray ionization mass spectrometer. Several parameters such as irradiation wavelength, irradiation time, mobile-phase composition, and pH as well as different organic acid modifiers are investigated, and their impact on the detection of the N-nitrosodialkylamine-acid complex and its dissociative photolysis products is presented here. Additionally, the type of structural information obtained from the photolytic processes of N-nitrosodialkylamines is compared to that obtained by using in-source collision-induced dissociation. To demonstrate the potential of this technique, six N-nitrosodialkylamines are studied to determine the limits of the response, the limits of detection and confirmation, and the reproducibility. The technique`s versatility is also exhibited by utilizing negative-ion mode as a complementary means for analysis of the compounds. Finally, an illustrative application for N-nitrosodimethylamine analysis in beer is described. 38 refs., 6 figs., 4 tabs.
- OSTI ID:
- 199361
- Journal Information:
- Analytical Chemistry (Washington), Vol. 68, Issue 3; Other Information: PBD: 1 Feb 1996
- Country of Publication:
- United States
- Language:
- English
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