Revealing the Bacterial Quorum-Sensing Effect on the Biofilm Formation of Diatom Cylindrotheca sp. Using Multimodal Imaging

Yang, Cuiyun; Song, Guojuan; Son, Jiyoung; Howard, Logan; Yu, Xiao-Ying

doi:10.3390/microorganisms11071841

Revealing the Bacterial Quorum-Sensing Effect on the Biofilm Formation of Diatom Cylindrotheca sp. Using Multimodal Imaging

Journal Article · Thu Jul 20 00:00:00 EDT 2023 · Microorganisms

DOI:https://doi.org/10.3390/microorganisms11071841· OSTI ID:1991702

Yang, Cuiyun ^[1]; Song, Guojuan ^[2]; ^[3]; Howard, Logan ^[4]; ^[4]

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Qingdao National Laboratory for Marine Science and Technology (China)
Qingdao National Laboratory for Marine Science and Technology (China); Yantai University (China)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Diatoms contribute to carbon fixation in the oceans by photosynthesis and always form biofouling organized by extracellular polymeric substances (EPS) in the marine environment. Bacteria-produced quorum-sensing signal molecules N-acyl homoserine lactones (AHLs) were found to play an important role in the development of Cylindrotheca sp. in previous studies, but the EPS composition change was unclear. This study used the technology of alcian blue staining and scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) to directly observe the biofilm formation process. The results showed that AHLs promote the growth rates of diatoms and the EPS secretion of biofilm components. AHLs facilitated the diatom-biofilm formation by a forming process dependent on the length of carbon chains. AHLs increased the biofilm thickness and the fluorescence intensity and then altered the three-dimensional (3D) structures of the diatom-biofilm. In addition, the enhanced EPS content in the diatom-biofilm testified that AHLs aided biofilm formation. This study provides a collection of new experimental evidence of the interaction between bacteria and microalgae in fouling biofilms.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC05-00OR22725; AC05-76RL01830

OSTI ID:: 1991702

Alternate ID(s):: OSTI ID: 2040077

Report Number(s):: PNNL-SA--155861

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 7 Vol. 11; ISSN 2076-2607

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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