Broadly neutralizing antibodies against sarbecoviruses generated by immunization of macaques with an AS03-adjuvanted COVID-19 vaccine

Feng, Yupeng; Yuan, Meng; Powers, John M.; Hu, Mengyun; Munt, Jennifer E.; Arunachalam, Prabhu S.; Leist, Sarah R.; Bellusci, Lorenza; Kim, JungHyun; Sprouse, Kaitlin R.; Adams, Lily E.; Sundaramurthy, Sumana; Zhu, Xueyong; Shirreff, Lisa M.; Mallory, Michael L.; Scobey, Trevor D.; Moreno, Alberto; O’Hagan, Derek T.; Kleanthous, Harry; Villinger, Francois J.; Veesler, David; King, Neil P.; Suthar, Mehul S.; Khurana, Surender; Baric, Ralph S.; Wilson, Ian A.; Pulendran, Bali

doi:10.1126/scitranslmed.adg7404

Broadly neutralizing antibodies against sarbecoviruses generated by immunization of macaques with an AS03-adjuvanted COVID-19 vaccine

Journal Article · Wed May 10 00:00:00 EDT 2023 · Science Translational Medicine

DOI:https://doi.org/10.1126/scitranslmed.adg7404· OSTI ID:1989359

^[1]; ^[2]; ^[3]; ^[4]; Munt, Jennifer E. ^[3]; ^[4]; ^[3]; ^[5]; ^[5]; ^[6]; ^[3]; ^[7]; ^[2]; ^[8]; ^[3]; Scobey, Trevor D. ^[3]; ^[9]; O’Hagan, Derek T. ^[10]; Kleanthous, Harry ^[11]; ^[8] more »

Stanford Univ., CA (United States); slac
Scripps Research Institute, La Jolla, CA (United States)
University of North Carolina, Chapel Hill, NC (United States)
Stanford Univ., CA (United States)
U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Biologics Evaluation and Research
Univ. of Washington, Seattle, WA (United States)
Sino Biological US, Inc. Wayne, PA (United States)
Univ. of Louisiana at Lafayette, New Iberia, LA (United States)
Emory Univ., Atlanta, GA (United States). School of Medicine
GSK, Rockville, MD (United States)
Bill and Melinda Gates Foundation, Seattle, WA (United States)
Univ. of Washington, Seattle, WA (United States). Howard Hughes Medical Institute
Emory National Primate Research Center, Atlanta, GA (United States)
Stanford Univ., CA (United States). School of Medicine

The rapid emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that evade immunity elicited by vaccination has placed an imperative on the development of countermeasures that provide broad protection against SARS-CoV-2 and related sarbecoviruses. Here, we identified extremely potent monoclonal antibodies (mAbs) that neutralized multiple sarbecoviruses from macaques vaccinated with AS03-adjuvanted monovalent subunit vaccines. Longitudinal analysis revealed progressive accumulation of somatic mutation in the immunoglobulin genes of antigen-specific memory B cells (MBCs) for at least 1 year after primary vaccination. Antibodies generated from these antigen-specific MBCs at 5 to 12 months after vaccination displayed greater potency and breadth relative to those identified at 1.4 months. Fifteen of the 338 (about 4.4%) antibodies isolated at 1.4 to 6 months after the primary vaccination showed potency against SARS-CoV-2 BA.1, despite the absence of serum BA.1 neutralization. 25F9 and 20A7 neutralized authentic clade 1 sarbecoviruses (SARS-CoV, WIV-1, SHC014, SARS-CoV-2 D614G, BA.1, and Pangolin-GD) and vesicular stomatitis virus–pseudotyped clade 3 sarbecoviruses (BtKY72 and PRD-0038). 20A7 and 27A12 showed potent neutralization against all SARS-CoV-2 variants and multiple Omicron sublineages, including BA.1, BA.2, BA.3, BA.4/5, BQ.1, BQ.1.1, and XBB. Crystallography studies revealed the molecular basis of broad and potent neutralization through targeting conserved sites within the RBD. Prophylactic protection of 25F9, 20A7, and 27A12 was confirmed in mice, and administration of 25F9 particularly provided complete protection against SARS-CoV-2, BA.1, SARS-CoV, and SHC014 challenge. These data underscore the extremely potent and broad activity of these mAbs against sarbecoviruses.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: Bill and Melinda Gates Foundation; National Cancer Institute (NCI); National Institute of Allergy and Infectious Diseases (NIAID); National Institute of General Medical Sciences (NIGMS); U.S. Food and Drug Administration (FDA); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-06CH11357; AC02-76SF00515

OSTI ID:: 1989359

Journal Information:: Science Translational Medicine, Journal Name: Science Translational Medicine Journal Issue: 695 Vol. 15; ISSN 1946-6234

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

References (75)

[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Structural insights into coronavirus entry Tortorici, M. Alejandra; Veesler, David Advances in Virus Research https://doi.org/10.1016/bs.aivir.2019.08.002	book	January 2019
A Therapeutic Non-self-reactive SARS-CoV-2 Antibody Protects from Lung Pathology in a COVID-19 Hamster Model Kreye, Jakob; Reincke, S. Momsen; Kornau, Hans-Christian Cell, Vol. 183, Issue 4 https://doi.org/10.1016/j.cell.2020.09.049	journal	November 2020
A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice Leist, Sarah R.; Dinnon, Kenneth H.; Schäfer, Alexandra Cell, Vol. 183, Issue 4 https://doi.org/10.1016/j.cell.2020.09.050	journal	November 2020
Maturation and persistence of the anti-SARS-CoV-2 memory B cell response Sokal, Aurélien; Chappert, Pascal; Barba-Spaeth, Giovanna Cell, Vol. 184, Issue 5 https://doi.org/10.1016/j.cell.2021.01.050	journal	March 2021
Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccines Walls, Alexandra C.; Miranda, Marcos C.; Schäfer, Alexandra Cell, Vol. 184, Issue 21 https://doi.org/10.1016/j.cell.2021.09.015	journal	October 2021
Alarming antibody evasion properties of rising SARS-CoV-2 BQ and XBB subvariants Wang, Qian; Iketani, Sho; Li, Zhiteng Cell, Vol. 186, Issue 2 https://doi.org/10.1016/j.cell.2022.12.018	journal	January 2023
Molecular analysis of a public cross-neutralizing antibody response to SARS-CoV-2 Yuan, Meng; Wang, Yiquan; Lv, Huibin Cell Reports, Vol. 41, Issue 7 https://doi.org/10.1016/j.celrep.2022.111650	journal	November 2022
Antibody Light-Chain-Restricted Recognition of the Site of Immune Pressure in the RV144 HIV-1 Vaccine Trial Is Phylogenetically Conserved Wiehe, Kevin; Easterhoff, David; Luo, Kan Immunity, Vol. 41, Issue 6 https://doi.org/10.1016/j.immuni.2014.11.014	journal	December 2014
Rhesus and cynomolgus macaque immunoglobulin heavy-chain genotyping yields comprehensive databases of germline VDJ alleles Vázquez Bernat, Néstor; Corcoran, Martin; Nowak, Izabela Immunity, Vol. 54, Issue 2 https://doi.org/10.1016/j.immuni.2020.12.018	journal	February 2021
Affinity maturation of SARS-CoV-2 neutralizing antibodies confers potency, breadth, and resilience to viral escape mutations Muecksch, Frauke; Weisblum, Yiska; Barnes, Christopher O. Immunity, Vol. 54, Issue 8 https://doi.org/10.1016/j.immuni.2021.07.008	journal	August 2021
Isolation of antibody V(D)J sequences from single cell sorted rhesus macaque B cells Sundling, Christopher; Phad, Ganesh; Douagi, Iyadh Journal of Immunological Methods, Vol. 386, Issue 1-2 https://doi.org/10.1016/j.jim.2012.09.003	journal	December 2012
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
ISCOMATRIX™ adjuvant promotes epitope spreading and antibody affinity maturation of influenza A H7N9 virus like particle vaccine that correlate with virus neutralization in humans Chung, Ka Yan; Coyle, Elizabeth M.; Jani, Dewal Vaccine, Vol. 33, Issue 32 https://doi.org/10.1016/j.vaccine.2015.06.047	journal	July 2015
Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor Ge, Xing-Yi; Li, Jia-Lu; Yang, Xing-Lou Nature, Vol. 503, Issue 7477 https://doi.org/10.1038/nature12711	journal	October 2013
A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence Menachery, Vineet D.; Yount, Boyd L.; Debbink, Kari Nature Medicine, Vol. 21, Issue 12 https://doi.org/10.1038/nm.3985	journal	November 2015
Cross-reactive immunity against the SARS-CoV-2 Omicron variant is low in pediatric patients with prior COVID-19 or MIS-C Tang, Juanjie; Novak, Tanya; Hecker, Julian Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-30649-1	journal	May 2022
Effectiveness of mRNA-1273 vaccination against SARS-CoV-2 omicron subvariants BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 Tseng, Hung Fu; Ackerson, Bradley K.; Bruxvoort, Katia J. Nature Communications, Vol. 14, Issue 1 https://doi.org/10.1038/s41467-023-35815-7	journal	January 2023
AS03-adjuvanted H5N1 vaccine promotes antibody diversity and affinity maturation, NAI titers, cross-clade H5N1 neutralization, but not H1N1 cross-subtype neutralization Khurana, Surender; Coyle, Elizabeth M.; Manischewitz, Jody npj Vaccines, Vol. 3, Issue 1 https://doi.org/10.1038/s41541-018-0076-2	journal	October 2018
“World in motion” – emulsion adjuvants rising to meet the pandemic challenges O’Hagan, Derek T.; van der Most, Robbert; Lodaya, Rushit N. npj Vaccines, Vol. 6, Issue 1 https://doi.org/10.1038/s41541-021-00418-0	journal	December 2021
Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic Boni, Maciej F.; Lemey, Philippe; Jiang, Xiaowei Nature Microbiology, Vol. 5, Issue 11 https://doi.org/10.1038/s41564-020-0771-4	journal	July 2020
Emerging concepts in the science of vaccine adjuvants Pulendran, Bali; S. Arunachalam, Prabhu; O’Hagan, Derek T. Nature Reviews Drug Discovery, Vol. 20, Issue 6 https://doi.org/10.1038/s41573-021-00163-y	journal	April 2021
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody Pinto, Dora; Park, Young-Jun; Beltramello, Martina Nature, Vol. 583, Issue 7815 https://doi.org/10.1038/s41586-020-2349-y	journal	May 2020
A mouse-adapted model of SARS-CoV-2 to test COVID-19 countermeasures Dinnon, Kenneth H.; Leist, Sarah R.; Schäfer, Alexandra Nature, Vol. 586, Issue 7830 https://doi.org/10.1038/s41586-020-2708-8	journal	August 2020
Addendum: A pneumonia outbreak associated with a new coronavirus of probable bat origin Zhou, Peng; Yang, Xing-Lou; Wang, Xian-Guang Nature, Vol. 588, Issue 7836 https://doi.org/10.1038/s41586-020-2951-z	journal	November 2020
Evolution of antibody immunity to SARS-CoV-2 Gaebler, Christian; Wang, Zijun; Lorenzi, Julio C. C. Nature, Vol. 591, Issue 7851 https://doi.org/10.1038/s41586-021-03207-w	journal	January 2021
mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants Wang, Zijun; Schmidt, Fabian; Weisblum, Yiska Nature, Vol. 592, Issue 7855 https://doi.org/10.1038/s41586-021-03324-6	journal	February 2021
Adjuvanting a subunit COVID-19 vaccine to induce protective immunity Arunachalam, Prabhu S.; Walls, Alexandra C.; Golden, Nadia Nature, Vol. 594, Issue 7862 https://doi.org/10.1038/s41586-021-03530-2	journal	April 2021
SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape Starr, Tyler N.; Czudnochowski, Nadine; Liu, Zhuoming Nature https://doi.org/10.1038/s41586-021-03807-6	journal	July 2021
Broad sarbecovirus neutralization by a human monoclonal antibody Tortorici, M. Alejandra; Czudnochowski, Nadine; Starr, Tyler N. Nature, Vol. 597, Issue 7874 https://doi.org/10.1038/s41586-021-03817-4	journal	July 2021
Hybrid immunity improves B cells and antibodies against SARS-CoV-2 variants Andreano, Emanuele; Paciello, Ida; Piccini, Giulia Nature, Vol. 600, Issue 7889 https://doi.org/10.1038/s41586-021-04117-7	journal	October 2021
Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift Cameroni, Elisabetta; Bowen, John E.; Rosen, Laura E. Nature, Vol. 602, Issue 7898 https://doi.org/10.1038/s41586-021-04386-2	journal	December 2021
Considerable escape of SARS-CoV-2 Omicron to antibody neutralization Planas, Delphine; Saunders, Nell; Maes, Piet Nature, Vol. 602, Issue 7898 https://doi.org/10.1038/s41586-021-04389-z	journal	December 2021
ACE2 binding is an ancestral and evolvable trait of sarbecoviruses Starr, Tyler N.; Zepeda, Samantha K.; Walls, Alexandra C. Nature, Vol. 603, Issue 7903 https://doi.org/10.1038/s41586-022-04464-z	journal	February 2022
Germinal centre-driven maturation of B cell response to mRNA vaccination Kim, Wooseob; Zhou, Julian Q.; Horvath, Stephen C. Nature, Vol. 604, Issue 7904 https://doi.org/10.1038/s41586-022-04527-1	journal	February 2022
BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection Cao, Yunlong; Yisimayi, Ayijiang; Jian, Fanchong Nature, Vol. 608, Issue 7923 https://doi.org/10.1038/s41586-022-04980-y	journal	June 2022
SARS-CoV-2 immune repertoire in MIS-C and pediatric COVID-19 Ravichandran, Supriya; Tang, Juanjie; Grubbs, Gabrielle Nature Immunology, Vol. 22, Issue 11 https://doi.org/10.1038/s41590-021-01051-8	journal	October 2021
Efficacy of a Fourth Dose of Covid-19 mRNA Vaccine against Omicron Regev-Yochay, Gili; Gonen, Tal; Gilboa, Mayan New England Journal of Medicine, Vol. 386, Issue 14 https://doi.org/10.1056/NEJMc2202542	journal	April 2022
Efficacy of Antibodies and Antiviral Drugs against Omicron BA.2.12.1, BA.4, and BA.5 Subvariants Takashita, Emi; Yamayoshi, Seiya; Simon, Viviana New England Journal of Medicine, Vol. 387, Issue 5 https://doi.org/10.1056/NEJMc2207519	journal	August 2022
Effectiveness and Durability of the BNT162b2 Vaccine against Omicron Sublineages in South Africa Collie, Shirley; Nayager, Jiren; Bamford, Lesley New England Journal of Medicine, Vol. 387, Issue 14 https://doi.org/10.1056/NEJMc2210093	journal	October 2022
Identification of a Novel Coronavirus in Patients with Severe Acute Respiratory Syndrome Drosten, Christian; Günther, Stephan; Preiser, Wolfgang New England Journal of Medicine, Vol. 348, Issue 20 https://doi.org/10.1056/NEJMoa030747	journal	May 2003
Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia Zaki, Ali M.; van Boheemen, Sander; Bestebroer, Theo M. New England Journal of Medicine, Vol. 367, Issue 19 https://doi.org/10.1056/NEJMoa1211721	journal	November 2012
The Severe Acute Respiratory Syndrome Peiris, Joseph S. M.; Yuen, Kwok Y.; Osterhaus, Albert D. M. E. New England Journal of Medicine, Vol. 349, Issue 25 https://doi.org/10.1056/NEJMra032498	journal	December 2003
SARS-like WIV1-CoV poised for human emergence Menachery, Vineet D.; Yount, Boyd L.; Sims, Amy C. Proceedings of the National Academy of Sciences, Vol. 113, Issue 11 https://doi.org/10.1073/pnas.1517719113	journal	March 2016
Adjuvanted H5N1 influenza vaccine enhances both cross-reactive memory B cell and strain-specific naive B cell responses in humans Ellebedy, Ali H.; Nachbagauer, Raffael; Jackson, Katherine J. L. Proceedings of the National Academy of Sciences, Vol. 117, Issue 30 https://doi.org/10.1073/pnas.1906613117	journal	July 2020
A broad and potent neutralization epitope in SARS-related coronaviruses Yuan, Meng; Zhu, Xueyong; He, Wan-ting Proceedings of the National Academy of Sciences, Vol. 119, Issue 29 https://doi.org/10.1073/pnas.2205784119	journal	June 2022
IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis Brochet, X.; Lefranc, M. -P.; Giudicelli, V. Nucleic Acids Research, Vol. 36, Issue Web Server https://doi.org/10.1093/nar/gkn316	journal	May 2008
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
A Highly Conserved Neutralizing Epitope on Group 2 Influenza A Viruses Ekiert, D. C.; Friesen, R. H. E.; Bhabha, G. Science, Vol. 333, Issue 6044 https://doi.org/10.1126/science.1204839	journal	July 2011
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV Yuan, Meng; Wu, Nicholas C.; Zhu, Xueyong Science, Vol. 368, Issue 6491 https://doi.org/10.1126/science.abb7269	journal	April 2020
Structural basis of a shared antibody response to SARS-CoV-2 Yuan, Meng; Liu, Hejun; Wu, Nicholas C. Science, Vol. 369, Issue 6507 https://doi.org/10.1126/science.abd2321	journal	July 2020
Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms Tortorici, M. Alejandra; Beltramello, Martina; Lempp, Florian A. Science, Vol. 370, Issue 6519 https://doi.org/10.1126/science.abe3354	journal	September 2020
Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody Rappazzo, C. Garrett; Tse, Longping V.; Kaku, Chengzi I. Science, Vol. 371, Issue 6531 https://doi.org/10.1126/science.abf4830	journal	February 2021
Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice Martinez, David R.; Schäfer, Alexandra; Leist, Sarah R. Science, Vol. 373, Issue 6558 https://doi.org/10.1126/science.abi4506	journal	August 2021
Broad betacoronavirus neutralization by a stem helix–specific human antibody Pinto, Dora; Sauer, Maximilian M.; Czudnochowski, Nadine Science, Vol. 373, Issue 6559 https://doi.org/10.1126/science.abj3321	journal	September 2021
Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models Cohen, Alexander A.; van Doremalen, Neeltje; Greaney, Allison J. Science, Vol. 377, Issue 6606 https://doi.org/10.1126/science.abq0839	journal	August 2022
ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies Low, Jun Siong; Jerak, Josipa; Tortorici, M. Alejandra Science, Vol. 377, Issue 6607 https://doi.org/10.1126/science.abq2679	journal	August 2022
Broadly neutralizing antibodies target the coronavirus fusion peptide Dacon, Cherrelle; Tucker, Courtney; Peng, Linghang Science, Vol. 377, Issue 6607 https://doi.org/10.1126/science.abq3773	journal	August 2022
Imprinted antibody responses against SARS-CoV-2 Omicron sublineages Park, Young-Jun; Pinto, Dora; Walls, Alexandra C. Science, Vol. 378, Issue 6620 https://doi.org/10.1126/science.adc9127	journal	November 2022
Prolonged evolution of the human B cell response to SARS-CoV-2 infection Sakharkar, Mrunal; Rappazzo, C. Garrett; Wieland-Alter, Wendy F. Science Immunology, Vol. 6, Issue 56 https://doi.org/10.1126/sciimmunol.abg6916	journal	February 2021
Vaccines with MF59 Adjuvant Expand the Antibody Repertoire to Target Protective Sites of Pandemic Avian H5N1 Influenza Virus Khurana, Surender; Chearwae, Wanida; Castellino, Flora Science Translational Medicine, Vol. 2, Issue 15 https://doi.org/10.1126/scitranslmed.3000624	journal	January 2010
MF59 Adjuvant Enhances Diversity and Affinity of Antibody-Mediated Immune Response to Pandemic Influenza Vaccines Khurana, Surender; Verma, Nitin; Yewdell, Jonathan W. Science Translational Medicine, Vol. 3, Issue 85 https://doi.org/10.1126/scitranslmed.3002336	journal	June 2011
Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits Ravichandran, Supriya; Coyle, Elizabeth M.; Klenow, Laura Science Translational Medicine, Vol. 12, Issue 550 https://doi.org/10.1126/scitranslmed.abc3539	journal	July 2020
Broadly neutralizing antibodies to SARS-related viruses can be readily induced in rhesus macaques He, Wan-ting; Yuan, Meng; Callaghan, Sean Science Translational Medicine, Vol. 14, Issue 657 https://doi.org/10.1126/scitranslmed.abl9605	journal	August 2022
Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine Arunachalam, Prabhu S.; Feng, Yupeng; Ashraf, Usama Science Translational Medicine, Vol. 14, Issue 658 https://doi.org/10.1126/scitranslmed.abq4130	journal	August 2022
Neutralisation of circulating SARS-CoV-2 delta and omicron variants by convalescent plasma and SARS-CoV-2 hyperimmune intravenous human immunoglobulins for treatment of COVID-19 Zahra, Fatema Tuz; Bellusci, Lorenza; Grubbs, Gabrielle Annals of the Rheumatic Diseases, Vol. 81, Issue 7 https://doi.org/10.1136/annrheumdis-2022-222115	journal	February 2022
A Mouse-Adapted SARS-Coronavirus Causes Disease and Mortality in BALB/c Mice Roberts, Anjeanette; Deming, Damon; Paddock, Christopher D. PLoS Pathogens, Vol. 3, Issue 1 https://doi.org/10.1371/journal.ppat.0030005	journal	January 2007
Clinical application of monoclonal antibodies in targeted therapy Superson, Maciej; Szmyt, Katarzyna European Journal of Clinical and Experimental Medicine, Vol. 17, Issue 4 https://doi.org/10.15584/ejcem.2019.4.9	journal	January 2020
A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice Baric, Ralph ImmPort https://doi.org/10.21430/m33hae2cn9	dataset	January 2023
Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine Suthar, Mehul; Wang, Taia; Grifoni, Alba ImmPort https://doi.org/10.21430/m3fbixlu6o	dataset	January 2023
Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody Gunn, Bonnie; Baric, Ralph ImmPort https://doi.org/10.21430/m3wrg2rj32	dataset	January 2023
Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies Zhang, Fan; Wang, Longyu; Niu, Xuefeng Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.02426	journal	October 2019

Similar Records

Broad SARS-CoV-2 neutralization by monoclonal and bispecific antibodies derived from a Gamma-infected individual

Journal Article · Thu Sep 21 20:00:00 EDT 2023 · iScience · OSTI ID:2469865

Targeting the Spike Receptor Binding Domain Class V Cryptic Epitope by an Antibody with Pan-Sarbecovirus Activity

Journal Article · Sun Jul 02 20:00:00 EDT 2023 · Journal of Virology · OSTI ID:2423689

A multi-specific, multi-affinity antibody platform neutralizes sarbecoviruses and confers protection against SARS-CoV-2 in vivo

Journal Article · Tue May 23 20:00:00 EDT 2023 · Science Translational Medicine · OSTI ID:2423266

Related Subjects

60 APPLIED LIFE SCIENCES

Broadly neutralizing antibodies against sarbecoviruses generated by immunization of macaques with an AS03-adjuvanted COVID-19 vaccine

Citation Formats

References (75)

Similar Records

Related Subjects