Microbiological quality, antibiotic resistant bacteria and relevant resistance genes in ready-to-eat Pacific oysters ( Magallana gigas )

Guedes, Bárbara; Godinho, Ofélia; Lage, Olga Maria; Quinteira, Sandra

doi:10.1093/femsle/fnad053

Microbiological quality, antibiotic resistant bacteria and relevant resistance genes in ready-to-eat Pacific oysters ( Magallana gigas )

Journal Article · Mon Jun 12 00:00:00 EDT 2023 · FEMS Microbiology Letters (Online)

DOI:https://doi.org/10.1093/femsle/fnad053· OSTI ID:1987991

; Godinho, Ofélia; Lage, Olga Maria;

Abstract

Oysters are a highly valued seafood but can endanger public health, if they are eaten raw or barely cooked. We evaluated the microbiological quality of Pacific oysters (Magallana gigas) by international standard methods in four groups (each with four to five animals) acquired from supermarkets and directly from a farm producer. Most of the groups presented satisfactory microbiological quality. In two groups of oysters, ‘questionable’ or ‘unsatisfactory’ quality was observed for the coagulase-positive Staphylococcus parameter. Culture-based methods did not detect Salmonella spp. or enteropathogenic Vibrio spp., but Vibrio alginolyticus, a potential foodborne pathogen, was identified by molecular analysis. Fifty strains, belonging to 19 species, were isolated in antibiotic-supplemented media, and their antibiotic susceptibility profile was evaluated. Genes coding for β-lactamases were searched by PCR in bacteria showing resistance phenotype. Decreased susceptibility or resistance to distinct antibiotics were observed for bacteria from depurated and non-depurated oysters. The blaTEM gene was identified in Escherichia fergusonii and Shigella dysenteriae strains, which showed multidrug-resistant phenotypes. The possibility that oysters might be a source of antibiotic-resistant bacteria/antibiotic resistance genes is of great concern and highlights the need for stricter controls and preventative measures to mitigate and counteract the dissemination of antibiotic resistance across the food chain.

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Sponsoring Organization:: USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain

OSTI ID:: 1987991

Journal Information:: FEMS Microbiology Letters (Online), Journal Name: FEMS Microbiology Letters (Online) Vol. 370; ISSN 1574-6968

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Netherlands

Language:: English

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