Anion exchange and extraction chromatography tandem column isolation of zirconium-89 (89Zr) from cyclotron bombarded targets using an automated fluidic platform

O'Hara, Matthew J.; Carter, Jennifer C.; Kellogg, Cynthia M.; Link, Jeanne M.

doi:10.1016/j.chroma.2022.463347

Title: Anion exchange and extraction chromatography tandem column isolation of zirconium-89 (⁸⁹Zr) from cyclotron bombarded targets using an automated fluidic platform

Journal Article · Sat Jul 16 00:00:00 EDT 2022 · Journal of Chromatography

DOI:https://doi.org/10.1016/j.chroma.2022.463347· OSTI ID:1987542

^[1]; Carter, Jennifer C. ^[1]; Kellogg, Cynthia M. ^[1]; Link, Jeanne M. ^[2]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Oregon Health & Science Univ., Portland, OR (United States)

The long-lived positron emitter ⁸⁹Zr is a highly promising nuclide employed in diagnostic Positron Emission Tomography (PET) imaging. Methods of radiochemical processing to obtain ⁸⁹Zr for clinical use are traditionally performed with a single hydroxamate resin column. In this work, we present a tandem column purification method for the preparation of high-purity ⁸⁹Zr from cyclotron bombarded Y metal foils. The primary column is a macroporous, strongly basic anion exchange resin on styrene divinylbenzene co-polymer. The secondary microcolumn, with an internal volume of 33 µL, is packed with an extraction chromatography resin (ExCR) loaded with HDEHP. A condition of “inverted selectivity” is presented, wherein the ⁸⁹Zr elution from the primary column is synonymous with the load condition on the secondary column. The ability to transfer ⁸⁹Zr from one column to the next allows two sequential column clean-up methods to be performed prior to the final elution of the ⁸⁹Zr product. This approach assures delivery of very high purity ⁸⁹Zr . The tandem column purification process has been implemented into a prototype automated fluidic system. Optimization of the method is presented, followed by evaluation of the process using seven cyclotron bombarded Y metal foil targets. Once optimized, we found that 93.7±2.3% of the ⁸⁹Zr present in the foils was recovered in the secondary column elution fraction (0.8 M oxalic acid). Radiochromatograms of the product elution peaks enabled determination of full width at half-maximum (FWHM) and ⁸⁹Zr collection yields as a function of volume. Because of the small size of the secondary microcolumn, a ⁸⁹Zr product volume of ~0.28 mL is reported, which provides a substantially increased nuclide concentration over traditional methods. Finally, we evaluated the transchelation of the resulting ⁸⁹Zr oxalate product to deferoxamine mesylate (DFOM) salt. We observed effective specific activities (ESA) and bindable metals concentrations ([M_B]) that exceed those reported by the traditional single hydroxamate column method.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830; S10-RR017229

OSTI ID:: 1987542

Alternate ID(s):: OSTI ID: 1960970

Report Number(s):: PNNL-SA-169036; TRN: US2402782

Journal Information:: Journal of Chromatography, Vol. 1678; ISSN 0021-9673

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
laboratory automation
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medical radionuclide
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Ln resin
extraction chromatography
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Title: Anion exchange and extraction chromatography tandem column isolation of zirconium-89 (89Zr) from cyclotron bombarded targets using an automated fluidic platform

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References (60)

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Title: Anion exchange and extraction chromatography tandem column isolation of zirconium-89 (⁸⁹Zr) from cyclotron bombarded targets using an automated fluidic platform