Analyzing BMP2, FGFR, and TGF Beta Expressions in High-Grade Osteosarcoma Untreated and Treated Autografts Using Proteomic Analysis

Madda, Rashmi; Chen, Chao-Ming; Chen, Cheng-Fong; Wang, Jir-You; Wu, Hsin-Yi; Wu, Po-Kuei; Chen, Wei-Ming

doi:10.3390/ijms23137409

Analyzing BMP2, FGFR, and TGF Beta Expressions in High-Grade Osteosarcoma Untreated and Treated Autografts Using Proteomic Analysis

Journal Article · Sun Jul 03 00:00:00 UTC 2022 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms23137409· OSTI ID:1987541

Madda, Rashmi ^[1]; ^[2]; ^[2]; Wang, Jir-You ^[2]; ^[3]; Wu, Po-Kuei ^[2]; Chen, Wei-Ming ^[2]

National Yang-Ming University (Taiwan); Taipei Veterans General Hospital (Taiwan); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
National Yang-Ming University (Taiwan); Taipei Veterans General Hospital (Taiwan)
National Taipei University (Taiwan)

In the last few decades, biological reconstruction techniques have improved greatly for treating high-grade osteosarcoma patients. To conserve the limb, and its function the affected tumor-bearing bones have been treated using liquid nitrogen and irradiation processes that enable the removal of entire tumors from the bone, and these treated autografts can be reconstructed for the patients. Here, we focus on the expressions of the growth factor family proteins from the untreated and treated autografts that play a crucial role in bone union, remodeling, and regeneration. In this proteomic study, we identify several important cytoskeletal, transcriptional, and growth factor family proteins that showed substantially low levels in untreated autografts. Interestingly, these protein expressions were elevated after treating the tumor-bearing bones using liquid nitrogen and irradiation. Therefore, from our preliminary findings, we chose to determine the expressions of BMP2, TGF-Beta, and FGFR proteins by the target proteomics approach. Using a newly recruited validation set, we successfully validate the expressions of the selected proteins. Furthermore, the increased growth factor protein expression after treatment with liquid nitrogen may contribute to bone regeneration healing, assist in faster recovery, and reduce local recurrence and metastatic spread in high-grade sarcoma patients.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1987541

Report Number(s):: PNNL-SA-178369

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 13 Vol. 23; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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60 APPLIED LIFE SCIENCES
BMP2
TGF-Beta
biological reconstruction
bone morphogenetic proteins
growth factors
mass spectrometry
osteosarcoma
target proteomics

Analyzing BMP2, FGFR, and TGF Beta Expressions in High-Grade Osteosarcoma Untreated and Treated Autografts Using Proteomic Analysis

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