Manipulation of glycogen and sucrose synthesis increases photosynthetic productivity in cyanobacteria

Cantrell, Michael; Cano, Melissa; Sebesta, Jacob; Paddock, Troy; Xiong, Wei; Chou, Katherine J.; Yu, Jianping

doi:10.3389/fmicb.2023.1124274

Manipulation of glycogen and sucrose synthesis increases photosynthetic productivity in cyanobacteria

Journal Article · Thu May 18 04:00:00 EDT 2023 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2023.1124274· OSTI ID:1986507

^[1]; Cano, Melissa ^[1]; Sebesta, Jacob ^[1]; Paddock, Troy ^[1]; ^[1]; ^[1]; ^[1]

National Renewable Energy Laboratory (NREL), Golden, CO (United States). Biosciences Center

Photosynthetic productivity is limited by low energy conversion efficiency in naturally evolved photosynthetic organisms, via multiple mechanisms that are not fully understood. Here we show evidence that extends recent findings that cyanobacteria use “futile” cycles in the synthesis and degradation of carbon compounds to dissipate ATP. Reduction of the glycogen cycle or the sucrose cycle in the model cyanobacterium Synechocystis 6803 led to redirection of cellular energy toward faster growth under simulated outdoor light conditions in photobioreactors that was accompanied by higher energy charge [concentration ratio of ATP/(ATP + ADP)]. Such manipulation of energy metabolism may have potential in engineering microalgal chassis cells to increase productivity of biomass or target metabolites.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE National Renewable Energy Laboratory (NREL), Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1986507

Report Number(s):: NREL/JA--2700-84835; MainId:85608; UUID:182dacb4-eb3e-47c8-9989-88fcccdf321c; MainAdminID:69841

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 14; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
ATP
cyanobacteria
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photosynthesis
sucrose

Manipulation of glycogen and sucrose synthesis increases photosynthetic productivity in cyanobacteria

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