Spray‐induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control

McRae, Amanda G.; Taneja, Jyoti; Yee, Kathleen; Shi, Xinyi; Haridas, Sajeet; LaButti, Kurt; Singan, Vasanth; Grigoriev, Igor V.; Wildermuth, Mary C.

doi:10.1111/mpp.13361

Title: Spray‐induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control

Journal Article · Tue Jun 20 00:00:00 EDT 2023 · Molecular Plant Pathology

DOI:https://doi.org/10.1111/mpp.13361· OSTI ID:1986094

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^[1]; Yee, Kathleen ^[1]; Shi, Xinyi ^[1];

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Department of Plant and Microbial Biology University of California Berkeley California USA
US Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley California USA
Department of Plant and Microbial Biology University of California Berkeley California USA, US Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley California USA

Abstract Spray‐induced gene silencing (SIGS) is an emerging tool for crop pest protection. It utilizes exogenously applied double‐stranded RNA to specifically reduce pest target gene expression using endogenous RNA interference machinery. In this study, SIGS methods were developed and optimized for powdery mildew fungi, which are widespread obligate biotrophic fungi that infect agricultural crops, using the known azole‐fungicide target cytochrome P450 51 (CYP51) in the Golovinomyces orontii–Arabidopsis thaliana pathosystem. Additional screening resulted in the identification of conserved gene targets and processes important to powdery mildew proliferation: apoptosis‐antagonizing transcription factor in essential cellular metabolism and stress response; lipid catabolism genes lipase a , lipase 1 , and acetyl‐CoA oxidase in energy production ; and genes involved in manipulation of the plant host via abscisic acid metabolism ( 9‐cis‐epoxycarotenoid dioxygenase , xanthoxin dehydrogenase , and a putative abscisic acid G‐protein coupled receptor ) and secretion of the effector protein, effector candidate 2 . Powdery mildew is the dominant disease impacting grapes and extensive powdery mildew resistance to applied fungicides has been reported. We therefore developed SIGS for the Erysiphe necator–Vitis vinifera system and tested six successful targets identified using the G. orontii–A. thaliana system. For all targets tested, a similar reduction in powdery mildew disease was observed between systems. This indicates screening of broadly conserved targets in the G. orontii–A. thaliana pathosystem identifies targets and processes for the successful control of other powdery mildew fungi. The efficacy of SIGS on powdery mildew fungi makes SIGS an exciting prospect for commercial powdery mildew control.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); American Vineyard Foundation; National Science Foundation (NSF); National Institute of Food and Agriculture

Grant/Contract Number:: DE‐AC02‐05CH11231; AC02-05CH11231; 2138; 2366; MCB-1617020; PFI-TT-1919244

OSTI ID:: 1986094

Alternate ID(s):: OSTI ID: 2229282

Journal Information:: Molecular Plant Pathology, Journal Name: Molecular Plant Pathology Vol. 24 Journal Issue: 9; ISSN 1464-6722

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
dsRNA
Erysiphe necator
Golovinomyces orontii
grapevine
powdery mildew
spray-induced gene silencing

Title: Spray‐induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control

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