Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation

Li, Chonglei; Du, Xiaoyu; Liu, Zhi-Hua; Li, Bing-Zhi; Meng, Xianzhi; Zhao, Ji; Zhao, Zhi-Min; Ragauskas, Arthur J.

doi:10.1016/j.ijbiomac.2023.123475

Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation

Journal Article · Fri Jan 27 23:00:00 EST 2023 · International Journal of Biological Macromolecules

DOI:https://doi.org/10.1016/j.ijbiomac.2023.123475· OSTI ID:1985376

Li, Chonglei ^[1]; Du, Xiaoyu ^[1]; Liu, Zhi-Hua ^[2]; Li, Bing-Zhi ^[2]; Meng, Xianzhi ^[3]; Zhao, Ji ^[1]; Zhao, Zhi-Min ^[4]; ^[5]

Inner Mongolia Univ., Hohhot (China)
Tianjin Univ. (China)
Univ. of Tennessee, Knoxville, TN (United States)
Inner Mongolia Univ., Hohhot (China); Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Steam explosion coupling high-temperature short-time sterilization (SE-HTST) was exploited to modify cellulosic biomass medium properties and promote high-solid fermentation (HSF). Biomass characterization analysis showed that SE-HTST enlarged microstructural pores and cavities in solid media, providing more effective space for microbial growth. Meanwhile, SE-HTST helped to release glucose from the cellulose with 35.8 ± 4.5, 20.0 ± 2.3, and 12.3 ± 5.7 mg glucose/g dry medium at 24, 48, and 72 h of fermentation, which were 3.1, 2.3, and 1.5 times higher than that in medium from conventional thermal sterilization (CTS), respectively. SE-HTST increased the viable cell and spore number of Bacillus subtilis by 1.8 and 1.6 times at 72 h of fermentation compared to CTS. Moreover, the expressions of master transcriptional gene spo0A and the early sigma factors of sigF and sigE genes gradually increased in the SE-HTST medium, showing enhanced sporulation in HSF. Therefore, SE-HTST is an effective strategy for facilitating cellulose degradation, improving glucose nutrients in biomass medium, and promoting sporulation-regulatory gene expression during high-solid fermentation, which enhances the production of microbial ecological agents using B. subtilis significantly.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1985376

Journal Information:: International Journal of Biological Macromolecules, Journal Name: International Journal of Biological Macromolecules Journal Issue: 1 Vol. 232; ISSN 0141-8130

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (32)

Thermal sterilization of heat‐sensitive products using high‐temperature short‐time sterilization Mann, Angelika; Kiefer, Markus; Leuenberger, Hans Journal of Pharmaceutical Sciences, Vol. 90, Issue 3 https://doi.org/10.1002/1520-6017(200103)90:3<275::AID-JPS3>3.0.CO;2-I	journal	March 2001
Biotechnological advantages of laboratory-scale solid-state fermentation with fungi H�lker, U.; H�fer, M.; Lenz, J. Applied Microbiology and Biotechnology, Vol. 64, Issue 2 https://doi.org/10.1007/s00253-003-1504-3	journal	April 2004
Development of putative probiotics as feed additives: validation in a porcine-specific gastrointestinal tract model Yeo, Soyoung; Lee, Suro; Park, Hyunjoon Applied Microbiology and Biotechnology, Vol. 100, Issue 23 https://doi.org/10.1007/s00253-016-7812-1	journal	September 2016
Progress in research and application development of surface display technology using Bacillus subtilis spores Lin, Ping; Yuan, Haibo; Du, Jieke Applied Microbiology and Biotechnology, Vol. 104, Issue 6 https://doi.org/10.1007/s00253-020-10348-x	journal	January 2020
Lactobacillus salivarius and Lactobacillus agilis feeding regulates intestinal stem cells activity by modulating crypt niche in hens Hong, Yi; Zhou, Zhou; Yu, Lingzi Applied Microbiology and Biotechnology, Vol. 105, Issue 23 https://doi.org/10.1007/s00253-021-11606-2	journal	October 2021
Cellulose–water interactions during enzymatic hydrolysis as studied by time domain NMR Felby, Claus; Thygesen, Lisbeth G.; Kristensen, Jan B. Cellulose, Vol. 15, Issue 5 https://doi.org/10.1007/s10570-008-9222-8	journal	May 2008
A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues Carra, Andrea; Gambino, Giorgio; Schubert, Andrea Analytical Biochemistry, Vol. 360, Issue 2 https://doi.org/10.1016/j.ab.2006.09.022	journal	January 2007
Effect of steam explosion and microbial fermentation on cellulose and lignin degradation of corn stover Chang, Juan; Cheng, Wei; Yin, Qingqiang Bioresource Technology, Vol. 104 https://doi.org/10.1016/j.biortech.2011.10.070	journal	January 2012
Ethanol production from glucose and xylose obtained from steam exploded water-extracted olive tree pruning using phosphoric acid as catalyst Negro, M. J.; Alvarez, C.; Ballesteros, I. Bioresource Technology, Vol. 153 https://doi.org/10.1016/j.biortech.2013.11.079	journal	February 2014
A novel steam explosion sterilization improving solid-state fermentation performance Zhao, Zhi-Min; Wang, Lan; Chen, Hong-Zhang Bioresource Technology, Vol. 192 https://doi.org/10.1016/j.biortech.2015.05.099	journal	September 2015
Physical structure changes of solid medium by steam explosion sterilization Zhao, Zhi-Min; Wang, Lan; Chen, Hong-Zhang Bioresource Technology, Vol. 203 https://doi.org/10.1016/j.biortech.2015.12.043	journal	March 2016
Crystalline reduction, surface area enlargement and pore generation of chitin by instant catapult steam explosion Tian, Zhiqing; Wang, Shikui; Hu, Xuefang Carbohydrate Polymers, Vol. 200 https://doi.org/10.1016/j.carbpol.2018.07.075	journal	November 2018
Correlation of porous structure, mass transfer and enzymatic hydrolysis of steam exploded corn stover Zhao, Junying; Chen, Hongzhang Chemical Engineering Science, Vol. 104 https://doi.org/10.1016/j.ces.2013.10.022	journal	December 2013
Inactivation of chemical and heat-resistant spores of Bacillus and Geobacillus by nitrogen cold atmospheric plasma evokes distinct changes in morphology and integrity of spores van Bokhorst-van de Veen, Hermien; Xie, Houyu; Esveld, Erik Food Microbiology, Vol. 45 https://doi.org/10.1016/j.fm.2014.03.018	journal	February 2015
Low-field NMR study of heat-induced gelation of pork myofibrillar proteins and its relationship with microstructural characteristics Han, Minyi; Wang, Peng; Xu, Xinglian Food Research International, Vol. 62 https://doi.org/10.1016/j.foodres.2014.05.062	journal	August 2014
pH-dependent RNA isolation from cells encapsulated in chitosan-based biomaterials Farrag, Mahmoud; Abri, Shahrzad; Leipzig, Nic D. International Journal of Biological Macromolecules, Vol. 146 https://doi.org/10.1016/j.ijbiomac.2019.12.263	journal	March 2020
Comparison of poly-γ-glutamic acid production between sterilized and non-sterilized solid-state fermentation using agricultural waste as substrates Fang, Junnan; Liu, Yang; Huan, ChenChen Journal of Cleaner Production, Vol. 255 https://doi.org/10.1016/j.jclepro.2020.120248	journal	May 2020
Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes Fimlaid, Kelly A.; Shen, Aimee Current Opinion in Microbiology, Vol. 24 https://doi.org/10.1016/j.mib.2015.01.006	journal	April 2015
Extraction enhancing mechanism of steam exploded Radix Astragali Sui, Wenjie; Chen, Hongzhang Process Biochemistry, Vol. 49, Issue 12 https://doi.org/10.1016/j.procbio.2014.08.010	journal	December 2014
Dark fermentative hydrogen production from pretreated lignocellulosic biomass: Effects of inhibitory byproducts and recent trends in mitigation strategies Basak, Bikram; Jeon, Byong-Hun; Kim, Tae Hyun Renewable and Sustainable Energy Reviews, Vol. 133 https://doi.org/10.1016/j.rser.2020.110338	journal	November 2020
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials Mohsin, Muhammad Zubair; Omer, Rabia; Huang, Jiaofang Synthetic and Systems Biotechnology, Vol. 6, Issue 3 https://doi.org/10.1016/j.synbio.2021.07.002	journal	September 2021
Study of Water Dynamics in the Soaking, Steaming, and Solid-State Fermentation of Glutinous Rice by LF-NMR: A Novel Monitoring Approach Li, Teng; Tu, Chuanhai; Rui, Xin Journal of Agricultural and Food Chemistry, Vol. 63, Issue 12 https://doi.org/10.1021/acs.jafc.5b00769	journal	March 2015
Characterization of Thermostable and Chimeric Enzymes via Isopeptide Bond-Mediated Molecular Cyclization Gao, De-Ying; Sun, Xiao-Bao; Liu, Ming-Qi Journal of Agricultural and Food Chemistry, Vol. 67, Issue 24 https://doi.org/10.1021/acs.jafc.9b01459	journal	June 2019
Bioactive Secondary Metabolites from Bacillus subtilis: A Comprehensive Review Kaspar, Felix; Neubauer, Peter; Gimpel, Matthias Journal of Natural Products, Vol. 82, Issue 7 https://doi.org/10.1021/acs.jnatprod.9b00110	journal	July 2019
Water Distribution in Tofu and Application of T₂ Relaxation Measurements in Determination of Tofu’s Water-Holding Capacity Li, Teng; Rui, Xin; Li, Wei Journal of Agricultural and Food Chemistry, Vol. 62, Issue 34 https://doi.org/10.1021/jf503427m	journal	August 2014
Crisscross regulation of cell-type-specific gene expression during development in B. subtilis Losick, Richard; Stragier, Patrick Nature, Vol. 355, Issue 6361 https://doi.org/10.1038/355601a0	journal	February 1992
Spore formation in B acillus subtilis: Bacillus subtilis sporulation Tan, Irene S.; Ramamurthi, Kumaran S. Environmental Microbiology Reports, Vol. 6, Issue 3 https://doi.org/10.1111/1758-2229.12130	journal	December 2013
Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals Setlow, P. Journal of Applied Microbiology, Vol. 101, Issue 3 https://doi.org/10.1111/j.1365-2672.2005.02736.x	journal	September 2006
SpoVG is an important regulator of sporulation and affects biofilm formation by regulating Spo0A transcription in Bacillus cereus 0–9 Huang, Qiubin; Zhang, Zhen; Liu, Qing BMC Microbiology, Vol. 21, Issue 1 https://doi.org/10.1186/s12866-021-02239-6	journal	June 2021
Physicochemical Characterization of Native and Steam Explosion Pretreated Saccharum spontaneum (Wild Sugarcane) Selvaraj, Aruna; Sriramulu, Gobikrishnan International Journal of Renewable Energy Development, Vol. 9, Issue 3 https://doi.org/10.14710/ijred.2020.30240	journal	June 2020
Physico-Chemical Conversion of Lignocellulose: Inhibitor Effects and Detoxification Strategies: A Mini Review Kim, Daehwan Molecules, Vol. 23, Issue 2 https://doi.org/10.3390/molecules23020309	journal	February 2018
Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy No authors listed Processes, Vol. 7, Issue 10 https://doi.org/10.3390/pr7100644	journal	September 2019

Similar Records

The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation

Journal Article · Tue Jan 27 23:00:00 EST 2015 · Microbiology and Molecular Biology Reviews · OSTI ID:1454688

Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation

Journal Article · Tue Dec 31 23:00:00 EST 2013 · Biotechnology for Biofuels · OSTI ID:1121195

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Bacillus subtilis
cellulose
cellulosic biomass
high-solid fermentation
sporulation
steam explosion

Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation

Citation Formats

References (32)

Similar Records

Related Subjects