The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases

Altae-Tran, Han; Kannan, Soumya; Demircioglu, F. Esra; Oshiro, Rachel; Nety, Suchita P.; McKay, Luke J.; Dlakić, Mensur; Inskeep, William P.; Makarova, Kira S.; Macrae, Rhiannon K.; Koonin, Eugene V.; Zhang, Feng

doi:10.1126/science.abj6856

The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases

Journal Article · Fri Oct 01 04:00:00 EDT 2021 · Science

DOI:https://doi.org/10.1126/science.abj6856· OSTI ID:1982955

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Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.; Thermal Biology Institute, Montana State University, Bozeman, MT 59717, USA.; Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.
Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.; Thermal Biology Institute, Montana State University, Bozeman, MT 59717, USA.
National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.

Tracing the origin of CRISPR-Cas

CRISPR-Cas systems have transformed genome editing and other biotechnologies; however, the broader origins and diversity of RNA-guided nucleases have largely remained unexplored. Altae-Tranet al. show that three distinct transposon-encoded proteins, IscB, IsrB, and TnpB, are naturally occurring, reprogrammable RNA-guided DNA nucleases (see the Perspective by Rousset and Sorek). In addition to identifying diverse guide-encoding mechanisms, the authors elucidate the evolutionary relationship between IsrB, IscB, and CRISPR-Cas9. Overall, these newly characterized systems, called OMEGA (for obligate mobile element–guided activity) systems, are found in all domains of life and may be harnessed for biotechnology development. —DJ

Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1982955

Journal Information:: Science, Journal Name: Science Journal Issue: 6563 Vol. 374; ISSN 0036-8075

Publisher:: AAAS

Country of Publication:: United States

Language:: English

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