Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions among patients in the DRC enrolled from 2017 to 2018

Journal Article · · Scientific Reports
 [1];  [1];  [1];  [1];  [2];  [3];  [3];  [4];  [5];  [1]
  1. Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States)
  2. University of Lubumbashi (Democratic Republic of the Congo)
  3. National Institute of Biomedical Research (Democratic Republic of the Congo)
  4. University of Kinshasa (Democratic Republic of the Congo)
  5. Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States); US President’s Malaria Initiative, Atlanta, GA (United States)
+ Show Author Affiliations
Rapid diagnostic tests (RDTs) detecting histidine-rich protein 2 (HRP2) and HRP3 are widely used throughout sub-Saharan Africa (SSA) to diagnose Plasmodium falciparum malaria. However, multiple SSA countries have reported pfhrp2 and pfhrp3 (pfhrp2/3) gene deletions. Blood samples (n = 1109) collected from patients with P. falciparum infection from six health facilities throughout the Democratic Republic of the Congo (DRC) from March 2017 to January 2018 were evaluated for pfhrp2/3 deletions. Samples were assayed for HRP2, pan-Plasmodium LDH (pLDH) and aldolase (pAldolase) antigens by bead-based multiplex antigen assay. Samples with low HRP2 concentration compared to pLDH and pAldolase antigens were selected for further pfhrp2/3 genotyping PCRs. The majority of blood samples (93.3%, 1035/1109) had high concentrations of the HRP2 antigen. Single deletions of pfhrp2 were identified in 0.27% (3/1109) of screened samples, with one sample from each of the Kapolowe, Mikalayi, and Rutshuru study sites. A pfhrp3 single deletion (0.09%, 1/1109) was found in the Kapolowe site. Dual pfhrp2 and pfhrp3 deletions were not observed. Due to, the low numbers of pfhrp2 deletions and the sporadic locations of these deletions, the use of HRP2-based RDTs appears to still be appropriate for these locations in DRC.
Research Organization:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
Centers for Disease Control and Prevention (CDC); USDOE Office of Science (SC)
Grant/Contract Number:
SC0014664
OSTI ID:
1982170
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 11; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

References (26)

HRP2: Transforming Malaria Diagnosis, but with Caveats journal February 2020
Plasmodium falciparum parasites with histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in two endemic regions of Kenya journal November 2017
Combination of Serological, Antigen Detection, and DNA Data for Plasmodium falciparum Provides Robust Geospatial Estimates for Malaria Transmission in Haiti journal May 2020
Analysis of false-negative rapid diagnostic tests for symptomatic malaria in the Democratic Republic of the Congo journal March 2021
Pfhrp2 -deleted Plasmodium falciparum parasites in the Democratic Republic of Congo: A national cross-sectional survey journal November 2016
Screening forPfhrp2/3-DeletedPlasmodium falciparum, Non-falciparum, and Low-Density Malaria Infections by a Multiplex Antigen Assay journal September 2018
Prevalence of pfhrp2 and pfhrp3 gene deletions in Puerto Lempira, Honduras journal January 2015
Multiplex malaria antigen detection by bead-based assay and molecular confirmation by PCR shows no evidence of Pfhrp2 and Pfhrp3 deletion in Haiti journal November 2019
Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma journal January 2020
First evidence of the deletion in the pfhrp2 and pfhrp3 genes in Plasmodium falciparum from Equatorial Guinea journal March 2020
Screening for malaria antigen and anti-malarial IgG antibody in forcibly-displaced Myanmar nationals: Cox’s Bazar district, Bangladesh, 2018 journal March 2020
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 journal August 2020
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania journal November 2020
A Large Proportion of P. falciparum Isolates in the Amazon Region of Peru Lack pfhrp2 and pfhrp3: Implications for Malaria Rapid Diagnostic Tests journal January 2010
Molecular Diagnosis of Malaria by Photo-Induced Electron Transfer Fluorogenic Primers: PET-PCR journal February 2013
Prevalence of pfhrp2 and/or pfhrp3 Gene Deletion in Plasmodium falciparum Population in Eight Highly Endemic States in India journal August 2016
Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control journal September 2016
Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests journal February 2017
One-step PCR: A novel protocol for determination of pfhrp2 deletion status in Plasmodium falciparum journal July 2020
Capacity Development through the US President’s Malaria Initiative–Supported Antimalarial Resistance Monitoring in Africa Network journal December 2017
Major Threat to Malaria Control Programs byPlasmodium falciparumLacking Histidine-Rich Protein 2, Eritrea journal March 2018
Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics journal June 2020
Rapid Screening for Non-falciparum Malaria in Elimination Settings Using Multiplex Antigen and Antibody Detection: Post Hoc Identification of Plasmodium malariae in an Infant in Haiti journal June 2021
Performance of pfHRP2 versus pLDH antigen rapid diagnostic tests for the detection of Plasmodium falciparum : a systematic review and meta-analysis journal January 2017
Multiplex malaria antigen detection by bead-based assay and molecular confirmation by PCR shows no evidence of Pfhrp2 and Pfhrp3 deletion in Haiti collection January 2019
Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania collection January 2021

Similar Records

Investigation of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions and performance of a rapid diagnostic test for identifying asymptomatic malaria infection in northern Ethiopia, 2015
Journal Article · Thu Mar 03 19:00:00 EST 2022 · Malaria Journal · OSTI ID:1904987
Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate
Journal Article · Sat Oct 16 20:00:00 EDT 2021 · Malaria Journal · OSTI ID:1983040
Symptomatic Plasmodium vivax Infection in Rwanda
Journal Article · Tue Jan 18 19:00:00 EST 2022 · Open Forum Infectious Diseases · OSTI ID:1982716

Related Subjects

60 APPLIED LIFE SCIENCES
epidemiology
genotype