A tailored fast thioacidolysis method incorporating multi-reaction monitoring mode of GC-MS for higher sensitivity on lignin monomer quantification
[1];
- State Key Laboratory of Pulp and Paper Engineering , School of Light Industry and Engineering, South China University of Technology , Guangzhou 510640 , China
- State Key Laboratory of Pulp and Paper Engineering , School of Light Industry and Engineering, South China University of Technology , Guangzhou 510640 , China; Department of Biochemistry and Great Lakes Bioenergy Research Center , The Wisconsin Energy Institute, University of Wisconsin , Madison , WI 53726 , USA
Abstract Thioacidolysis is widely used for lignin structural characterization by cleaving β-aryl ethers to release syringyl (S), guaiacyl (G), andp-hydroxyphenyl (H) monomers followed by GC analysis. However, the traditional thioacidolysis method requires tedious extraction steps with chlorinated solvent underlying harmful to health, limiting its efficiency and application. Herein, an improved thioacidolysis method with high sensitivity for the quantitation of lignin-derived monomers was developed. The improved protocol used a quick, streamlined procedure to recover the monomeric products using ethyl acetate as extracting solvent and MS detector in multi-reaction monitoring mode to enhance its ability to detect extremely low concentration (0.1 ppb with signal-to-noise higher than 2) of monomeric products. Additionally, a fast GC program was established to speed up the GC quantitation. Several representative lignocellulose samples, including gymnosperm, angiosperm, and poaceae, were used to test this tailored method. The results demonstrated that the ratios of lignin monomer compositions determined by this method were consistent with that of traditional procedure despite the slightly higher monomer yields measured. More importantly, this method uses non-chlorinated solvent for microscale extraction and requires no evaporation step for workup, which is a green and efficient way for the quantification of lignin monomer compositions.
- Research Organization:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- SC0018409
- OSTI ID:
- 1980996
- Journal Information:
- Holzforschung, Vol. 76, Issue 7; ISSN 0018-3830
- Publisher:
- de Gruyter
- Country of Publication:
- United States
- Language:
- English
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