Biofilm disruption enhances growth rate and carbohydrate-active enzyme production in anaerobic fungi

Leggieri, Patrick A.; Valentine, Megan T.; O'Malley, Michelle A.

doi:10.1016/j.biortech.2022.127361

Biofilm disruption enhances growth rate and carbohydrate-active enzyme production in anaerobic fungi

Journal Article · Sat May 21 00:00:00 UTC 2022 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2022.127361· OSTI ID:1976892

Leggieri, Patrick A. ^[1]; Valentine, Megan T. ^[2]; O'Malley, Michelle A. ^[3]

University of California, Santa Barbara, CA (United States); OSTI
University of California, Santa Barbara, CA (United States)
University of California, Santa Barbara, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)

Anaerobic gut fungi (AGF) are lignocellulose degraders that naturally form biofilms in the rumen of large herbivores and in standard culture techniques. While biofilm formation enhances biomass degradation and carbohydrate-active enzyme (CAZyme) production in some bacteria and aerobic fungi, gene expression and metabolism in AGF biofilms have not been compared to non-biofilm cultures. Here, using the tunable morphology of the non-rhizoidal AGF, Caecomyces churrovis, the impacts of biofilm formation on AGF gene expression, metabolic flux, growth rate, and xylan degradation rate are quantified to inform future industrial scale-up efforts. Contrary to previous findings, C. churrovis upregulated catabolic CAZymes in stirred culture relative to biofilm culture. Using a de novo transcriptome, 197 new transcripts with predicted CAZyme function were identified. Stirred cultures grew and degraded xylan significantly faster than biofilm-forming cultures with negligible differences in primary metabolic flux, offering a way to accelerate AGF biomass valorization without altering the fermentation product profile. The rhizoidal AGF, Neocallimastix lanati, also grew faster with stirring on a solid plant substrate, suggesting that the advantages of stirred C. churrovis cultures may apply broadly to other AGF.

View Accepted Manuscript (DOE)

Research Organization:: University of California, Santa Barbara, CA (United States)

Sponsoring Organization:: Army Research Office (ARO); California NanoSystems Institute (CNSI); USDOE Office of Science (SC)

Grant/Contract Number:: SC0020420

OSTI ID:: 1976892

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 358; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
anaerobic fungi
biofilms
bioprocessing
differential expression
lignocellulose

Biofilm disruption enhances growth rate and carbohydrate-active enzyme production in anaerobic fungi

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