Interactions of xenobiotics with steroid hormone receptors and the sex-steroid binding protein in spotted seatrout
- Univ. of Texas, Port Aransas, TX (United States). Marine Science Inst.
- Univ. of California, Berkeley, CA (United States). Dept. of Environmental Science, Policy and Management
A variety of xenobiotics, such as DDT, methoxychlor and PCB mixtures and Kepone have estrogenic actions and disrupt reproduction in mammals by binding to nuclear estrogen receptors (ER). These xenobiotics were tested for their ability to bind to the hepatic ER of a marine fish, spotted seatrout (Cynoscion nebulosus). Several of the DDT derivatives, Kepone and PCB mixtures also bound to the seatrout ER over a range of 10{sup {minus}5}--10{sup {minus}3}M. Moreover, Kepone was shown to have both estrogenic and antiestrogenic actions in an in vitro liver slice vitellogenesis assay. These estrogenic compounds were also tested for their ability to bind to nuclear and plasma membrane progestogen (20{beta}-S) receptors in ovarian tissues and to the sex-steroid binding protein in seatrout plasma. Kepone, methoxychlor and o,p{prime}-DDT caused concentration dependent displacement of {sup 3}H2O{beta}-S from its plasma membrane receptor and inhibition of 20{beta}-S induced final maturation in an in vitro assay over the range of 10{sup {minus}7}--10{sup {minus}3}M, but did not alter steroid binding to the nuclear progestogen receptor. Significant binding of methoxychlor and the other organochlorines to the sex steroid binding protein was also observed. It is concluded from these studies that a variety of xenobiotics with estrogenic actions can also bind to other steroid receptors and binding proteins to influence other endocrine-mediated processes.
- OSTI ID:
- 197547
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9612%%321
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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