Novel candidate genes for lignin structure identified through genome-wide association study of naturally varying Populus trichocarpa

Bryant, Nathan; Zhang, Jin; Feng, Kai; Shu, Mengjun; Ployet, Raphael; Chen, Jin-Gui; Muchero, Wellington; Yoo, Chang Geun; Tschaplinski, Timothy J.; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.3389/fpls.2023.1153113

Title: Novel candidate genes for lignin structure identified through genome-wide association study of naturally varying Populus trichocarpa

Journal Article · Fri May 05 00:00:00 EDT 2023 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2023.1153113· OSTI ID:1972744

Bryant, Nathan; Zhang, Jin; Feng, Kai; Shu, Mengjun; Ployet, Raphael; Chen, Jin-Gui; Muchero, Wellington; Yoo, Chang Geun; Tschaplinski, Timothy J.; Pu, Yunqiao; Ragauskas, Arthur J.

Populus is a promising lignocellulosic feedstock for biofuels and bioproducts. However, the cell wall biopolymer lignin is a major barrier in conversion of biomass to biofuels. To investigate the variability and underlying genetic basis of the complex structure of lignin, a population of 409 three-year-old, naturally varying Populus trichocarpa genotypes were characterized by heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR). A subsequent genome-wide association study (GWAS) was conducted using approximately 8.3 million single nucleotide polymorphisms (SNPs), which identified 756 genes that were significantly associated (−log ₁₀ ( p -value)>6) with at least one lignin phenotype. Several promising candidate genes were identified, many of which have not previously been reported to be associated with lignin or cell wall biosynthesis. These results provide a resource for gaining insights into the molecular mechanisms of lignin biosynthesis and new targets for future genetic improvement in poplar.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1972744

Alternate ID(s):: OSTI ID: 1984342

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 14; ISSN 1664-462X

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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