Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

Gionfriddo, Caitlin M.; Soren, Ally Bullock; Wymore, Ann M.; Hartnett, D. Sean; Podar, Mircea; Parks, Jerry M.; Elias, Dwayne A.; Gilmour, Cynthia C.

doi:10.1128/aem.01768-22

Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

Journal Article · Thu Mar 23 00:00:00 EDT 2023 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.01768-22· OSTI ID:1972577

^[1]; Soren, Ally Bullock ^[1]; Wymore, Ann M. ^[2]; Hartnett, D. Sean ^[1]; ^[2]; ^[2]; Elias, Dwayne A. ^[3]; ^[1]

Smithsonian Environmental Research Center, Edgewater, MD (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Elias Consulting, LLC, Knoxville, TN (United States)

The hgcAB gene pair encodes mercury (Hg) methylation capability in a diverse group of microorganisms, but its evolution and transcriptional regulation remain unknown. Working from the possibility that the evolutionary function of HgcAB may not be Hg methylation, we test a possible link to arsenic resistance. Using model Hg methylator Pseudodesulfovibrio mercurii ND132, we evaluated transcriptional control of hgcAB by a putative ArsR encoded upstream and cotranscribed with hgcAB. This regulator shares homology with ArsR repressors of arsenic resistance and S-adenosylhomocysteine (SAH)- responsive regulators of methionine biosynthesis but is distinct from other ArsR/SahR proteins in P. mercurii. Using quantitative PCR (qPCR) and RNA sequencing (RNA-seq) transcriptome analyses, we confirmed this ArsR regulates hgcAB transcription and is responsive to arsenic and SAH. Additionally, RNA-seq indicated a possible link between hgcAB activity and arsenic transformations, with significant upregulation of other ArsRregulated arsenic resistance operons alongside hgcAB. Interestingly, wild-type ND132 was less sensitive to As(V) (but not As(III)) than an hgcAB knockout strain, supporting the idea that hgcAB may be linked to arsenic resistance. Arsenic significantly impacted rates of Hg methylation by ND132; however, responses varied with culture conditions. Differences in growth and metabolic activity did not account for arsenic impacts on methylation. While arsenic significantly increased hgcAB expression, hgcAB gene and transcript abundance was not a good predictor of Hg methylation rates. Taken together, these results support the idea that Hg and As cycling are linked in P. mercurii ND132. Our results may hold clues to the evolution of hgcAB and the controls on Hg methylation in nature. This work reveals a link between microbial mercury methylation and arsenic resistance and may hold clues to the evolution of mercury methylation genes (hgcAB). Microbes with hgcAB produce methylmercury, a strong neurotoxin that readily accumulates in the food web. This study addresses a critical gap in our understanding about the environmental factors that control hgcAB expression. We show that hgcAB expression is controlled by an ArsR-like regulator responsive to both arsenic and S-adenosylhomocysteine in our model organism, Pseudodesulfovibrio mercurii ND132. Exposure to arsenic also significantly impacted Pseudodesulfovibrio mercurii ND132 mercury methylation rates. However, expression of hgcAB was not always a good predictor of Hg methylation rates, highlighting the roles of Hg bioavailability and other biochemical mechanisms in methylmercury production. This study improves our understanding of the controls on hgcAB expression, which is needed to better predict environmental methylmercury production

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1972577

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 4 Vol. 89; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (91)

Methyltransferase: An enzyme assay for microbial methylmercury formation in acidic soils and sediments Siciliano, Steven D.; Lean, David R. S. Environmental Toxicology and Chemistry, Vol. 21, Issue 6 https://doi.org/10.1002/etc.5620210610	journal	June 2002
Biotic formation of methylmercury: A bio–physico–chemical conundrum Bravo, Andrea G.; Cosio, Claudia Limnology and Oceanography, Vol. 65, Issue 5 https://doi.org/10.1002/lno.11366	journal	November 2019
Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes Qian, Chen; Chen, Hongmei; Johs, Alexander PROTEOMICS, Vol. 18, Issue 17 https://doi.org/10.1002/pmic.201700479	journal	August 2018
Paludibacter jiangxiensis sp. nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field Qiu, Yan-Ling; Kuang, Xiao-Zhu; Shi, Xiao-Shuang Archives of Microbiology, Vol. 196, Issue 3 https://doi.org/10.1007/s00203-013-0951-1	journal	January 2014
Relationships between bacterial energetic metabolism, mercury methylation potential, and hgcA/hgcB gene expression in Desulfovibrio dechloroacetivorans BerOc1 Goñi-Urriza, Marisol; Corsellis, Yannick; Lanceleur, Laurent Environmental Science and Pollution Research, Vol. 22, Issue 18 https://doi.org/10.1007/s11356-015-4273-5	journal	March 2015
Effect of exogenous and endogenous sulfide on the production and the export of methylmercury by sulfate-reducing bacteria Barrouilhet, Sophie; Monperrus, Mathilde; Tessier, Emmanuel Environmental Science and Pollution Research, Vol. 30, Issue 2 https://doi.org/10.1007/s11356-022-22173-y	journal	August 2022
Mössbauer, EPR, and optical studies of the corrinoid/iron-sulfur protein involved in the synthesis of acetyl coenzyme A by Clostridium thermoaceticum. Ragsdale, S. W.; Lindahl, P. A.; Münck, E. Journal of Biological Chemistry, Vol. 262, Issue 29 https://doi.org/10.1016/S0021-9258(18)47936-7	journal	October 1987
The SmtB/ArsR family of metalloregulatory transcriptional repressors: structural insights into prokaryotic metal resistance Busenlehner, Laura S.; Pennella, Mario A.; Giedroc, David P. FEMS Microbiology Reviews, Vol. 27, Issue 2-3 https://doi.org/10.1016/S0168-6445(03)00054-8	journal	June 2003
Respiratory arsenate reductase as a bidirectional enzyme Richey, Christine; Chovanec, Peter; Hoeft, Shelley E. Biochemical and Biophysical Research Communications, Vol. 382, Issue 2 https://doi.org/10.1016/j.bbrc.2009.03.045	journal	May 2009
Vitamin B 12 in the spotlight again Bridwell-Rabb, Jennifer; Drennan, Catherine L. Current Opinion in Chemical Biology, Vol. 37 https://doi.org/10.1016/j.cbpa.2017.01.013	journal	April 2017
Joint estimation of biogeochemical model parameters from multiple experiments: A bayesian approach applied to mercury methylation Rathore, Saubhagya S.; Schwartz, Grace E.; Brooks, Scott C. Environmental Modelling & Software, Vol. 155 https://doi.org/10.1016/j.envsoft.2022.105453	journal	September 2022
Structures of two ArsR As(III)-responsive transcriptional repressors: Implications for the mechanism of derepression Prabaharan, Chandrasekaran; Kandavelu, Palani; Packianathan, Charles Journal of Structural Biology, Vol. 207, Issue 2 https://doi.org/10.1016/j.jsb.2019.05.009	journal	August 2019
Genome insights of mercury methylation among Desulfovibrio and Pseudodesulfovibrio strains Goñi-Urriza, Marisol; Klopp, Christophe; Ranchou-Peyruse, Magali Research in Microbiology, Vol. 171, Issue 1 https://doi.org/10.1016/j.resmic.2019.10.003	journal	January 2020
Mercury cycling in freshwater systems - An updated conceptual model Branfireun, Brian A.; Cosio, Claudia; Poulain, Alexandre J. Science of The Total Environment, Vol. 745 https://doi.org/10.1016/j.scitotenv.2020.140906	journal	November 2020
The pyruvate dehydrogenase multi-enzyme complex from Gram-negative bacteria de Kok, Aart; Hengeveld, Annechien F.; Martin, Alejandro Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1385, Issue 2 https://doi.org/10.1016/s0167-4838(98)00079-x	journal	June 1998
The Structure of an As(III) S-Adenosylmethionine Methyltransferase with 3-Coordinately Bound As(III) Depicts the First Step in Catalysis Packianathan, Charles; Kandavelu, Palani; Rosen, Barry P. Biochemistry, Vol. 57, Issue 28 https://doi.org/10.1021/acs.biochem.8b00457	journal	June 2018
Mercury Methylation Genes Identified across Diverse Anaerobic Microbial Guilds in a Eutrophic Sulfate-Enriched Lake Peterson, Benjamin D.; McDaniel, Elizabeth A.; Schmidt, Anna G. Environmental Science & Technology, Vol. 54, Issue 24 https://doi.org/10.1021/acs.est.0c05435	journal	November 2020
Expression Levels of hgcAB Genes and Mercury Availability Jointly Explain Methylmercury Formation in Stratified Brackish Waters Capo, Eric; Feng, Caiyan; Bravo, Andrea G. Environmental Science & Technology, Vol. 56, Issue 18 https://doi.org/10.1021/acs.est.2c03784	journal	September 2022
Linking Microbial Activities and Low-Molecular-Weight Thiols to Hg Methylation in Biofilms and Periphyton from High-Altitude Tropical Lakes in the Bolivian Altiplano Bouchet, Sylvain; Goñi-Urriza, Marisol; Monperrus, Mathilde Environmental Science & Technology, Vol. 52, Issue 17 https://doi.org/10.1021/acs.est.8b01885	journal	July 2018
Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations Christensen, Geoff A.; Gionfriddo, Caitlin M.; King, Andrew J. Environmental Science & Technology, Vol. 53, Issue 15 https://doi.org/10.1021/acs.est.8b06389	journal	July 2019
Role of ArsEFG in Roxarsone and Nitarsone Detoxification and Resistance Chen, Jian; Zhang, Jun; Rosen, Barry P. Environmental Science & Technology, Vol. 53, Issue 11 https://doi.org/10.1021/acs.est.9b01187	journal	May 2019
Overlooked Role of Putative Non-Hg Methylators in Predicting Methylmercury Production in Paddy Soils Liu, Yu-Rong; Yang, Ziming; Zhou, Xinquan Environmental Science & Technology, Vol. 53, Issue 21 https://doi.org/10.1021/acs.est.9b03013	journal	October 2019
Cobalamin-Dependent Methionine Synthase Is a Modular Protein with Distinct Regions for Binding Homocysteine, Methyltetrahydrofolate, Cobalamin, and Adenosylmethionine ^† Goulding, Celia W.; Postigo, Dellma; Matthews, Rowena G. Biochemistry, Vol. 36, Issue 26 https://doi.org/10.1021/bi9705164	journal	July 1997
The Influence of Sulfide on Solid-Phase Mercury Bioavailability for Methylation by Pure Cultures of Desulfobulbus propionicus (1pr3) Benoit, J. M.; Gilmour, C. C.; Mason, R. P. Environmental Science & Technology, Vol. 35, Issue 1 https://doi.org/10.1021/es001415n	journal	January 2001
A Quantitative Relationship that Demonstrates Mercury Methylation Rates in Marine Sediments Are Based on the Community Composition and Activity of Sulfate-Reducing Bacteria King, Jeffrey K.; Kostka, Joel E.; Frischer, Marc E. Environmental Science & Technology, Vol. 35, Issue 12 https://doi.org/10.1021/es001813q	journal	June 2001
Cobalt Limitation of Growth and Mercury Methylation in Sulfate-Reducing Bacteria Ekstrom, Eileen B.; Morel, François M. M. Environmental Science & Technology, Vol. 42, Issue 1, p. 93-99 https://doi.org/10.1021/es0705644	journal	January 2008
Dissolved Organic Matter Enhances Microbial Mercury Methylation Under Sulfidic Conditions Graham, Andrew M.; Aiken, George R.; Gilmour, Cynthia C. Environmental Science & Technology, Vol. 46, Issue 5 https://doi.org/10.1021/es203658f	journal	February 2012
Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review Hsu-Kim, Heileen; Kucharzyk, Katarzyna H.; Zhang, Tong Environmental Science & Technology, Vol. 47, Issue 6 https://doi.org/10.1021/es304370g	journal	February 2013
Mercury Methylation by Novel Microorganisms from New Environments Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L. Environmental Science & Technology, Vol. 47, Issue 20 https://doi.org/10.1021/es403075t	journal	September 2013
Mercury Methylation by HgcA: Theory Supports Carbanion Transfer to Hg(II) Zhou, Jing; Riccardi, Demian; Beste, Ariana Inorganic Chemistry, Vol. 53, Issue 2 https://doi.org/10.1021/ic401992y	journal	December 2013
Characterization and transcription of arsenic respiration and resistance genes during in situ uranium bioremediation Giloteaux, Ludovic; Holmes, Dawn E.; Williams, Kenneth H. The ISME Journal, Vol. 7, Issue 2 https://doi.org/10.1038/ismej.2012.109	journal	October 2012
Relationships between protein-encoding gene abundance and corresponding process are commonly assumed yet rarely observed Rocca, Jennifer D.; Hall, Edward K.; Lennon, Jay T. The ISME Journal, Vol. 9, Issue 8 https://doi.org/10.1038/ismej.2014.252	journal	December 2014
StringTie enables improved reconstruction of a transcriptome from RNA-seq reads Pertea, Mihaela; Pertea, Geo M.; Antonescu, Corina M. Nature Biotechnology, Vol. 33, Issue 3 https://doi.org/10.1038/nbt.3122	journal	February 2015
KBase: The United States Department of Energy Systems Biology Knowledgebase Arkin, Adam P.; Cottingham, Robert W.; Henry, Christopher S. Nature Biotechnology, Vol. 36, Issue 7 https://doi.org/10.1038/nbt.4163	journal	July 2018
Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system Anantharaman, Karthik; Brown, Christopher T.; Hug, Laura A. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms13219	journal	October 2016
Molecular mechanism of energy conservation in polysulfide respiration Jormakka, Mika; Yokoyama, Ken; Yano, Takahiro Nature Structural & Molecular Biology, Vol. 15, Issue 7 https://doi.org/10.1038/nsmb.1434	journal	June 2008
Mercury methylation by metabolically versatile and cosmopolitan marine bacteria Lin, Heyu; Ascher, David B.; Myung, Yoochan The ISME Journal, Vol. 15, Issue 6 https://doi.org/10.1038/s41396-020-00889-4	journal	January 2021
Genome-centric view of carbon processing in thawing permafrost Woodcroft, Ben J.; Singleton, Caitlin M.; Boyd, Joel A. Nature, Vol. 560, Issue 7716 https://doi.org/10.1038/s41586-018-0338-1	journal	July 2018
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype Kim, Daehwan; Paggi, Joseph M.; Park, Chanhee Nature Biotechnology, Vol. 37, Issue 8 https://doi.org/10.1038/s41587-019-0201-4	journal	August 2019
ColabFold: making protein folding accessible to all Mirdita, Milot; Schütze, Konstantin; Moriwaki, Yoshitaka Nature Methods, Vol. 19, Issue 6 https://doi.org/10.1038/s41592-022-01488-1	journal	May 2022
Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation Cooper, Connor J.; Zheng, Kaiyuan; Rush, Katherine W. Communications Biology, Vol. 3, Issue 1 https://doi.org/10.1038/s42003-020-1047-5	journal	June 2020
Arsenate decreases production of methylmercury across increasing sulfate concentration amendments in freshwater lake sediments Mija, Aždajić; Jules M., Blais; Alexandre J., Poulain Environmental Science: Processes & Impacts, Vol. 24, Issue 9 https://doi.org/10.1039/D1EM00543J	journal	January 2022
Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S -adenosylmethionine methyltransferase Qin, Jie; Rosen, Barry P.; Zhang, Yang Proceedings of the National Academy of Sciences, Vol. 103, Issue 7 https://doi.org/10.1073/pnas.0506836103	journal	February 2006
Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria Schaefer, J. K.; Rocks, S. S.; Zheng, W. Proceedings of the National Academy of Sciences, Vol. 108, Issue 21 https://doi.org/10.1073/pnas.1105781108	journal	May 2011
Structural and mechanistic analysis of the arsenate respiratory reductase provides insight into environmental arsenic transformations Glasser, Nathaniel R.; Oyala, Paul H.; Osborne, Thomas H. Proceedings of the National Academy of Sciences, Vol. 115, Issue 37 https://doi.org/10.1073/pnas.1807984115	journal	August 2018
Unraveling the inner workings of respiratory arsenate reductase Stolz, John F.; Basu, Partha Proceedings of the National Academy of Sciences, Vol. 115, Issue 37 https://doi.org/10.1073/pnas.1812841115	journal	August 2018
The Role of Arsenic-Thiol Interactions in Metalloregulation of the ars Operon Shi, Weiping; Dong, Jun; Scott, Robert A. Journal of Biological Chemistry, Vol. 271, Issue 16, p. 9291-9297 https://doi.org/10.1074/jbc.271.16.9291	journal	April 1996
Trimmomatic: a flexible trimmer for Illumina sequence data Bolger, Anthony M.; Lohse, Marc; Usadel, Bjoern Bioinformatics, Vol. 30, Issue 15 https://doi.org/10.1093/bioinformatics/btu170	journal	April 2014
Gene Graphics: a genomic neighborhood data visualization web application Harrison, Katherine J.; Crécy-Lagard, Valérie de; Zallot, Rémi Bioinformatics, Vol. 34, Issue 8 https://doi.org/10.1093/bioinformatics/btx793	journal	December 2017
MMseqs2 desktop and local web server app for fast, interactive sequence searches Mirdita, Milot; Steinegger, Martin; Söding, Johannes Bioinformatics, Vol. 35, Issue 16 https://doi.org/10.1093/bioinformatics/bty1057	journal	January 2019
The microbial genomics of arsenic Andres, Jérémy; Bertin, Philippe N. FEMS Microbiology Reviews, Vol. 40, Issue 2 https://doi.org/10.1093/femsre/fuv050	journal	January 2016
The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update Afgan, Enis; Baker, Dannon; Batut, Bérénice Nucleic Acids Research, Vol. 46, Issue W1 https://doi.org/10.1093/nar/gky379	journal	May 2018
MGnify: the microbiome analysis resource in 2020 Mitchell, Alex L.; Almeida, Alexandre; Beracochea, Martin Nucleic Acids Research https://doi.org/10.1093/nar/gkz1035	journal	November 2019
Pseudodesulfovibrio hydrargyri sp. nov., a mercury-methylating bacterium isolated from a brackish sediment Ranchou-Peyruse, Magali; Goñi-Urriza, Marisol; Guignard, Marion International Journal of Systematic and Evolutionary Microbiology, Vol. 68, Issue 5 https://doi.org/10.1099/ijsem.0.002173	journal	May 2018
Pseudodesulfovibrio mercurii sp. nov., a mercury-methylating bacterium isolated from sediment Gilmour, Cynthia C.; Soren, Ally Bullock; Gionfriddo, Caitlin M. International Journal of Systematic and Evolutionary Microbiology, Vol. 71, Issue 3 https://doi.org/10.1099/ijsem.0.004697	journal	June 2019
Protein complex prediction with AlphaFold-Multimer Evans, Richard; O???Neill, Michael; Pritzel, Alexander https://doi.org/10.1101/2021.10.04.463034	preprint	March 2022
Control of methionine metabolism by the SahR transcriptional regulator in Proteobacteria: Control of methionine metabolism in Proteobacteria Novichkov, Pavel S.; Li, Xiaoqing; Kuehl, Jennifer V. Environmental Microbiology, Vol. 16, Issue 1 https://doi.org/10.1111/1462-2920.12273	journal	October 2013
Geomicrobiology of the carbon, nitrogen and sulphur cycles in Powell Lake: a permanently stratified water column containing ancient seawater Haas, Sebastian; Desai, Dhwani K.; LaRoche, Julie Environmental Microbiology, Vol. 21, Issue 10 https://doi.org/10.1111/1462-2920.14743	journal	July 2019
Anaerobic guilds responsible for mercury methylation in boreal wetlands of varied trophic status serving as either a methylmercury source or sink Schaefer, Jeffra K.; Kronberg, Rose‐Marie; Björn, Erik Environmental Microbiology, Vol. 22, Issue 9 https://doi.org/10.1111/1462-2920.15134	journal	July 2020
Elemental sulfur reduction by a deep‐sea hydrothermal vent Campylobacterium Sulfurimonas sp. NW10 Wang, Shasha; Jiang, Lijing; Hu, Qitao Environmental Microbiology, Vol. 23, Issue 2 https://doi.org/10.1111/1462-2920.15247	journal	October 2020
A consensus protocol for the recovery of mercury methylation genes from metagenomes Capo, Eric; Peterson, Benjamin D.; Kim, Minjae Molecular Ecology Resources, Vol. 23, Issue 1 https://doi.org/10.1111/1755-0998.13687	journal	August 2022
ArsP: a methylarsenite efflux permease Chen, Jian; Madegowda, Mahendra; Bhattacharjee, Hiranmoy Molecular Microbiology, Vol. 98, Issue 4 https://doi.org/10.1111/mmi.13145	journal	August 2015
Global prevalence and distribution of genes and microorganisms involved in mercury methylation Podar, Mircea; Gilmour, Cynthia C.; Brandt, Craig C. Science Advances, Vol. 1, Issue 9 https://doi.org/10.1126/sciadv.1500675	journal	October 2015
The Genetic Basis for Bacterial Mercury Methylation Parks, J. M.; Johs, A.; Podar, M. Science, Vol. 339, Issue 6125, p. 1332-1335 https://doi.org/10.1126/science.1230667	journal	February 2013
Genome-Resolved Metagenomics and Detailed Geochemical Speciation Analyses Yield New Insights into Microbial Mercury Cycling in Geothermal Springs Gionfriddo, Caitlin M.; Stott, Matthew B.; Power, Jean F. Applied and Environmental Microbiology, Vol. 86, Issue 15 https://doi.org/10.1128/AEM.00176-20	journal	July 2020
Site-Directed Mutagenesis of HgcA and HgcB Reveals Amino Acid Residues Important for Mercury Methylation Smith, Steven D.; Bridou, Romain; Johs, Alexander Applied and Environmental Microbiology, Vol. 81, Issue 9 https://doi.org/10.1128/AEM.00217-15	journal	February 2015
Kinetics of Enzymatic Mercury Methylation at Nanomolar Concentrations Catalyzed by HgcAB Date, Swapneeta S.; Parks, Jerry M.; Rush, Katherine W. Applied and Environmental Microbiology, Vol. 85, Issue 13 https://doi.org/10.1128/AEM.00438-19	journal	April 2019
Expression of Genes and Proteins Involved in Arsenic Respiration and Resistance in Dissimilatory Arsenate-Reducing Geobacter sp. Strain OR-1 Tsuchiya, Tatsuya; Ehara, Ayaka; Kasahara, Yasuhiro Applied and Environmental Microbiology, Vol. 85, Issue 14 https://doi.org/10.1128/AEM.00763-19	journal	May 2019
Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park Jay, Z. J.; Beam, J. P.; Dohnalkova, A. Applied and Environmental Microbiology, Vol. 81, Issue 17 https://doi.org/10.1128/AEM.01095-15	journal	June 2015
Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species Graham, Andrew M.; Bullock, Allyson L.; Maizel, Andrew C. Applied and Environmental Microbiology, Vol. 78, Issue 20, p. 7337-7346 https://doi.org/10.1128/AEM.01792-12	journal	August 2012
Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation Gilmour, Cynthia C.; Elias, Dwayne A.; Kucken, Amy M. Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 3938-3951 https://doi.org/10.1128/AEM.02993-10	journal	April 2011
Sulfate-Reducing Bacteria Methylate Mercury at Variable Rates in Pure Culture and in Marine Sediments King, J. K.; Kostka, J. E.; Frischer, M. E. Applied and Environmental Microbiology, Vol. 66, Issue 6, p. 2430-2437 https://doi.org/10.1128/AEM.66.6.2430-2437.2000	journal	June 2000
Genome Sequence of the Mercury-Methylating Strain Desulfovibrio desulfuricans ND132 Brown, S. D.; Gilmour, C. C.; Kucken, A. M. Journal of Bacteriology, Vol. 193, Issue 8, p. 2078-2079 https://doi.org/10.1128/JB.00170-11	journal	February 2011
The Electron Transfer System of Syntrophically Grown Desulfovibrio vulgaris Walker, C. B.; He, Z.; Yang, Z. K. Journal of Bacteriology, Vol. 191, Issue 18 https://doi.org/10.1128/JB.00356-09	journal	July 2009
Characterization of the Arsenate Respiratory Reductase from Shewanella sp. Strain ANA-3 Malasarn, D.; Keeffe, J. R.; Newman, D. K. Journal of Bacteriology, Vol. 190, Issue 1 https://doi.org/10.1128/JB.01110-07	journal	October 2007
Regulation of Arsenate Resistance in Desulfovibrio desulfuricans G20 by an arsRBCC Operon and an arsC Gene Li, X.; Krumholz, L. R. Journal of Bacteriology, Vol. 189, Issue 10 https://doi.org/10.1128/JB.01913-06	journal	March 2007
Metabolic Pathways Leading to Mercury Methylation in Desulfovibrio desulfuricans LS † Choi, Sung-Chan; Chase, Theodore; Bartha, Richard Applied and Environmental Microbiology, Vol. 60, Issue 11 https://doi.org/10.1128/aem.60.11.4072-4077.1994	journal	January 1994
Enzymatic catalysis of mercury methylation by Desulfovibrio desulfuricans LS. Choi, S. C.; Chase, T.; Bartha, R. Applied and Environmental Microbiology, Vol. 60, Issue 4 https://doi.org/10.1128/aem.60.4.1342-1346.1994	journal	January 1994
Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system Oppermann, F. B.; Schmidt, B.; Steinbüchel, A. Journal of Bacteriology, Vol. 173, Issue 2 https://doi.org/10.1128/jb.173.2.757-767.1991	journal	January 1991
Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system Oppermann, F. B.; Steinbüchel, A. Journal of Bacteriology, Vol. 176, Issue 2 https://doi.org/10.1128/jb.176.2.469-485.1994	journal	January 1994
Robust Mercury Methylation across Diverse Methanogenic Archaea Gilmour, Cynthia C.; Bullock, Allyson L.; McBurney, Alyssa mBio, Vol. 9, Issue 2 https://doi.org/10.1128/mBio.02403-17	journal	April 2018
Expanded Phylogenetic Diversity and Metabolic Flexibility of Mercury-Methylating Microorganisms McDaniel, Elizabeth A.; Peterson, Benjamin D.; Stevens, Sarah L. R. mSystems, Vol. 5, Issue 4 https://doi.org/10.1128/mSystems.00299-20	journal	August 2020
Shining light on recent advances in microbial mercury cycling Grégoire, Daniel S.; Poulain, Alexandre J. FACETS, Vol. 3, Issue 1 https://doi.org/10.1139/facets-2018-0015	journal	October 2018
Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization Zhang, Yan; Rodionov, Dmitry A.; Gelfand, Mikhail S. BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-78	journal	January 2009
Growthcurver: an R package for obtaining interpretable metrics from microbial growth curves Sprouffske, Kathleen; Wagner, Andreas BMC Bioinformatics, Vol. 17, Issue 1 https://doi.org/10.1186/s12859-016-1016-7	journal	April 2016
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Love, Michael I.; Huber, Wolfgang; Anders, Simon Genome Biology, Vol. 15, Issue 12 https://doi.org/10.1186/s13059-014-0550-8	journal	December 2014
Comparative Genomics of Transcriptional Regulation of Methionine Metabolism in Proteobacteria Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D. PLoS ONE, Vol. 9, Issue 11 https://doi.org/10.1371/journal.pone.0113714	journal	November 2014
An Improved hgcAB Primer Set and Direct High-Throughput Sequencing Expand Hg-Methylator Diversity in Nature Gionfriddo, Caitlin M.; Wymore, Ann M.; Jones, Daniel S. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.541554	journal	October 2020
Introducing the ArsR-Regulated Arsenic Stimulon Rawle, Rachel; Saley, Tara C.; Kang, Yoon-Suk Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.630562	journal	March 2021
Interactions with Arsenic: Mechanisms of Toxicity and Cellular Resistance in Eukaryotic Microorganisms De Francisco, Patricia; Martín-González, Ana; Rodriguez-Martín, Daniel International Journal of Environmental Research and Public Health, Vol. 18, Issue 22 https://doi.org/10.3390/ijerph182212226	journal	November 2021

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Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes

Journal Article · Wed Aug 15 20:00:00 EDT 2018 · Proteomics · OSTI ID:1468234

Contrary effects of phytoplankton Chlorella vulgaris and its exudates on mercury methylation by iron- and sulfate-reducing bacteria

Journal Article · Thu Mar 31 20:00:00 EDT 2022 · Journal of Hazardous Materials · OSTI ID:1866695

Pseudodesulfovibrio mercurii sp. nov., a mercury-methylating bacterium isolated from sediment

Journal Article · Wed Feb 10 19:00:00 EST 2021 · International Journal of Systematic and Evolutionary Microbiology · OSTI ID:1767872

Related Subjects

RNA-seq
59 BASIC BIOLOGICAL SCIENCES
ArsR
ars operon
arsenic
arsenic resistance
hgcAB
mercury
mercury biogeochemistry
methylmercury

RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 batch cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561189 The current record is supplemented by this dataset	dataset	January 2023
RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 washed-cell cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561387 The current record is supplemented by this dataset	dataset	January 2023
RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 washed-cell cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561387.v1 The current record is supplemented by this dataset	dataset	January 2023

Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

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