Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases

Chaudhury, Debayan; Torkelson, Ella R.; Meyers, Kaya A.; Acheson, Justin F.; Landucci, Leta; Pu, Yucen; Sun, Zimou; Tonelli, Marco; Bingman, Craig A.; Smith, Rebecca A.; Karlen, Steven D.; Mansfield, Shawn D.; Ralph, John; Fox, Brian G.

doi:10.1002/cbic.202300001

Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases

Journal Article · Wed Apr 12 00:00:00 EDT 2023 · ChemBioChem: a European journal of chemical biology

DOI:https://doi.org/10.1002/cbic.202300001· OSTI ID:1969598

^[1]; Torkelson, Ella R. ^[1]; Meyers, Kaya A. ^[1]; ^[1]; ^[1]; ^[2]; ^[2]; ^[1]; ^[1]; ^[1]; ^[1]; ^[2]; ^[1]; ^[1]

Department of Biochemistry University of Wisconsin-Madison 433 Babcock Drive Madison WI 53706 USA
Department of Botany and Department of Wood Science University of British Columbia 2424 Main Mall Vancouver BC V6T 1Z4 Canada

Abstract

Chemically labile ester linkages can be introduced into lignin by incorporation of monolignol conjugates, which are synthesized in planta by acyltransferases that use a coenzyme A (CoA) thioester donor and a nucleophilic monolignol alcohol acceptor. The presence of these esters facilitates processing and aids in the valorization of renewable biomass feedstocks. However, the effectiveness of this strategy is potentially limited by the low steady‐state levels of aromatic acid thioester donors in plants. As part of an effort to overcome this, aromatic acid CoA ligases involved in microbial aromatic degradation were identified and screened against a broad panel of substituted cinnamic and benzoic acids involved in plant lignification. Functional fingerprinting of this ligase library identified four robust, highly active enzymes capable of facile, rapid, and high‐yield synthesis of aromatic acid CoA thioesters under mild aqueous reaction conditions mimicking in planta activity.

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Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States); Univ. of Wisconsin-Madison, WI (United States); University of Wisconsin-Madison, WI (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: National Magnetic Resonance Facility at UW-Madison

Grant/Contract Number:: SC0018409; SC0020349

OSTI ID:: 1969598

Alternate ID(s):: OSTI ID: 2524627
OSTI ID: 1970261
OSTI ID: 1983527

Journal Information:: ChemBioChem: a European journal of chemical biology, Journal Name: ChemBioChem: a European journal of chemical biology Journal Issue: 9 Vol. 24; ISSN 1439-4227

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: France

Language:: English

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Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases

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