Designing 1D multiheme peptide amphiphile assemblies reminiscent of natural systems

Christopher Fry, H.; Divan, Ralu; Liu, Yuzi

doi:10.1039/d2nr00473a

Designing 1D multiheme peptide amphiphile assemblies reminiscent of natural systems

Journal Article · Tue Jun 28 00:00:00 EDT 2022 · Nanoscale

DOI:https://doi.org/10.1039/d2nr00473a· OSTI ID:1969401

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Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials

We discuss protein assemblies that bind and organize ordered arrays of cofactors yield function structures. Multiheme assemblies found in nature yield electronically conductivity 1D nanoscale fibers and are employed in anaerobic respiration. To understand the fundamental characteristics of these organized arrays, the design of peptide amphiphiles that assemble into 1D nanostructures and yield metalloporphyrin binding sites is presented. One challenge with this class of peptide amphiphiles is identifying the correct sequence composition for high affinity binding with high heme density. Here, the peptide c16-AH(Kx)n-CO₂H is explored to identify the impact of sequence length (n) and amino acid identity (x = L, I, or F) on binding affinity and midpoint potential. When n = 2, the peptide assembly yields the greatest affinity. The resulting nanoscale assemblies yield ordered arrays of the redox active molecule heme and have potential utility in the development of supramolecular bioelectronic materials useful in sensing as well as the development of enzymatic materials.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1969401

Alternate ID(s):: OSTI ID: 1875018

Journal Information:: Nanoscale, Journal Name: Nanoscale Journal Issue: 28 Vol. 14; ISSN 2040-3364

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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