Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications

Yuen Jr, John Se Kit; Saad, Michael K.; Xiang, Ning; Barrick, Brigid M.; DiCindio, Hailey; Li, Chunmei; Zhang, Sabrina W.; Rittenberg, Miriam; Lew, Emily T.; Zhang, Kevin Lin; Leung, Glenn; Pietropinto, Jaymie A.; Kaplan, David L.

doi:10.7554/eLife.82120

Title: Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications

Journal Article · Tue Apr 04 00:00:00 EDT 2023 · eLife

DOI:https://doi.org/10.7554/eLife.82120· OSTI ID:1968063

; Saad, Michael K.; Xiang, Ning; Barrick, Brigid M.; DiCindio, Hailey; Li, Chunmei; Zhang, Sabrina W.; Rittenberg, Miriam; Lew, Emily T.; Zhang, Kevin Lin; Leung, Glenn; Pietropinto, Jaymie A.;

We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production.

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Sponsoring Organization:: USDOE Advanced Research Projects Agency - Energy (ARPA-E)

Grant/Contract Number:: AR0001233

OSTI ID:: 1968063

Alternate ID(s):: OSTI ID: 1968065

Journal Information:: eLife, Journal Name: eLife Vol. 12; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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