Glycosylation of SARS-CoV-2: structural and functional insights

Shajahan, Asif; Pepi, Lauren E.; Rouhani, Daniel S.; Heiss, Christian; Azadi, Parastoo

doi:10.1007/s00216-021-03499-x

Glycosylation of SARS-CoV-2: structural and functional insights

Journal Article · Wed Jul 07 00:00:00 EDT 2021 · Analytical and Bioanalytical Chemistry

DOI:https://doi.org/10.1007/s00216-021-03499-x· OSTI ID:1963808

Shajahan, Asif ^[1]; Pepi, Lauren E. ^[2]; Rouhani, Daniel S. ^[2]; Heiss, Christian ^[2]; Azadi, Parastoo ^[2]

Univ. of Georgia, Athens, GA (United States); Complex Carbohydrate Research Center, University of Georgia
Univ. of Georgia, Athens, GA (United States)

The COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Similar to other coronaviruses, its particles are composed of four structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins. S, E, and M proteins are glycosylated, and the N protein is phosphorylated. The S protein is involved in the interaction with the host receptor human angiotensin-converting enzyme 2 (hACE2), which is also heavily glycosylated. Recent studies have revealed several other potential host receptors or factors that can increase or modulate the SARS-CoV-2 infection. Interestingly, most of these molecules bear carbohydrate residues. While glycans acquired by the viruses through the hijacking of the host machinery help the viruses in their infectivity, they also play roles in immune evasion or modulation. Here, glycans play complex roles in viral pathobiology, both on their own and in association with carrier biomolecules, such as proteins or glycosaminoglycans (GAGs). Understanding these roles in detail can help in developing suitable strategies for prevention and therapy of COVID-19. In this review, we sought to emphasize the interplay of SARS-CoV-2 glycosylated proteins and their host receptors in viral attachment, entry, replication, and infection. Moreover, the implications for future therapeutic interventions targeting these glycosylated biomolecules are also discussed in detail.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0015662

OSTI ID:: 1963808

Journal Information:: Analytical and Bioanalytical Chemistry, Journal Name: Analytical and Bioanalytical Chemistry Journal Issue: 29 Vol. 413; ISSN 1618-2642

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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The Hepatitis C Virus Glycan Shield and Evasion of the Humoral Immune Response Helle, François; Duverlie, Gilles; Dubuisson, Jean Viruses, Vol. 3, Issue 10 https://doi.org/10.3390/v3101909	journal	October 2011
Playing Hide and Seek: How Glycosylation of the Influenza Virus Hemagglutinin Can Modulate the Immune Response to Infection Tate, Michelle; Job, Emma; Deng, Yi-Mo Viruses, Vol. 6, Issue 3 https://doi.org/10.3390/v6031294	journal	March 2014
Toward Animal Cell Culture–Based Influenza Vaccine Design: Viral Hemagglutinin N-Glycosylation Markedly Impacts Immunogenicity Hütter, Julia; Rödig, Jana V.; Höper, Dirk The Journal of Immunology, Vol. 190, Issue 1 https://doi.org/10.4049/jimmunol.1201060	journal	January 2013
COVID ‐19 acute respiratory distress syndrome ( ARDS ): clinical features and differences from typical pre‐ COVID ‐19 ARDS Gibson, Peter G.; Qin, Ling; Puah, Ser Hon Medical Journal of Australia, Vol. 213, Issue 2 https://doi.org/10.5694/mja2.50674	journal	June 2020
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59 BASIC BIOLOGICAL SCIENCES
SARS-CoV-2 glycosylation
envelope protein glycosylation
glycosaminoglycan SARS-CoV-2
human ACE2 SARS-CoV-2
membrane protein glycosylation
spike protein glycosylation

Glycosylation of SARS-CoV-2: structural and functional insights

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