Structure of Lipopolysaccharide from Liberibacter crescens Is Low Molecular Weight and Offers Insight into Candidatus Liberibacter Biology

Black, Ian M.; Heiss, Christian; Jain, Mukesh; Muszyński, Artur; Carlson, Russell W.; Gabriel, Dean W.; Azadi, Parastoo

doi:10.3390/ijms222011240

Structure of Lipopolysaccharide from Liberibacter crescens Is Low Molecular Weight and Offers Insight into Candidatus Liberibacter Biology

Journal Article · Mon Oct 18 00:00:00 EDT 2021 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms222011240· OSTI ID:1963261

Black, Ian M. ^[1]; Heiss, Christian ^[2]; ^[3]; ^[2]; Carlson, Russell W. ^[2]; Gabriel, Dean W. ^[3]; Azadi, Parastoo ^[2]

Univ. of Georgia, Athens, GA (United States); Complex Carbohydrate Research Center, University of Georgia
Univ. of Georgia, Athens, GA (United States)
Univ. of Florida, Gainesville, FL (United States)

Huanglongbing (HLB) disease, also known as citrus greening disease, was first reported in the US in 2005. Since then, the disease has decimated the citrus industry in Florida, resulting in billions of dollars in crop losses and the destruction of thousands of acres of citrus groves. The causative agent of citrus greening disease is the phloem limited pathogen Candidatus Liberibacter asiaticus. As it has not been cultured, very little is known about the structural biology of the organism. Liberibacter are part of the Rhizobiaceae family, which includes nitrogen-fixing symbionts of legumes as well as the Agrobacterium plant pathogens. To better understand the Liberibacter genus, a closely related culturable bacterium (Liberibacter crescens or Lcr) has attracted attention as a model organism for structural and functional genomics of Liberibacters. Given that the structure of lipopolysaccharides (LPS) from Gram-negative bacteria plays a crucial role in mediating host-pathogen interactions, we sought to characterize the LPS from Lcr. We found that the major lipid A component of the LPS consisted of a pentaacylated molecule with a β-6-GlcN disaccharide backbone lacking phosphate. The polysaccharide portion of the LPS was unusual compared to previously described members of the Rhizobiaceae family in that it contained ribofuranosyl residues. The LPS structure presented here allows us to extrapolate known LPS structure/function relationships to members of the Liberibacter genus which cannot yet be cultured. It also offers insights into the biology of the organism and how they manage to effectively attack citrus trees.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0015662

OSTI ID:: 1963261

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 20 Vol. 22; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Structure of Lipopolysaccharide from Liberibacter crescens Is Low Molecular Weight and Offers Insight into Candidatus Liberibacter Biology

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