Transcriptome Mining Provides Insights into Cell Wall Metabolism and Fiber Lignification in Agave tequilana Weber

Maceda-López, Luis F.; Góngora-Castillo, Elsa B.; Ibarra-Laclette, Enrique; Morán-Velázquez, Dalia C.; Girón Ramírez, Amaranta; Bourdon, Matthieu; Villalpando-Aguilar, José L.; Toomer, Gabriela; Tang, John Z.; Azadi, Parastoo; Santamaría, Jorge M.; López-Rosas, Itzel; López, Mercedes G.; Simpson, June; Alatorre-Cobos, Fulgencio

doi:10.3390/plants11111496

Title: Transcriptome Mining Provides Insights into Cell Wall Metabolism and Fiber Lignification in Agave tequilana Weber

Journal Article · Thu Jun 02 00:00:00 EDT 2022 · Plants

DOI:https://doi.org/10.3390/plants11111496· OSTI ID:1962853

^[1];

^[2];

^[3]; Morán-Velázquez, Dalia C. ^[1]; Girón Ramírez, Amaranta ^[2]; Bourdon, Matthieu ^[4]; Villalpando-Aguilar, José L. ^[1];

^[5];

^[6]; Azadi, Parastoo ^[6]; Santamaría, Jorge M. ^[2]; López-Rosas, Itzel ^[1]; López, Mercedes G. ^[7]; Simpson, June ^[7];

^[1]

Colegio De Postgraduados Campus Campeche (Mexico)
Centro de Investigación Científica de Yucatán (CICY) (Mexico)
Instituto de Ecología A. C. (Mexico)
Univ. of Cambridge (United Kingdom)
Illinois Institute of Technology, Chicago, IL (United States)
Univ. of Georgia, Athens, GA (United States)
National Polytechnic Inst. (IPN), Zacatenco (Mexico). Centre for Research and Advanced Studies (CINVESTAV)

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioenergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for understanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots.

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Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0015662

OSTI ID:: 1962853

Journal Information:: Plants, Vol. 11, Issue 11; ISSN 2223-7747

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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