Engineered hosts with exogenous ligninase and uses thereof

Singh, Seema; Varman, Arul Mozhy

Title: Engineered hosts with exogenous ligninase and uses thereof

Patent · Tue Sep 27 00:00:00 EDT 2022

OSTI ID:1925142

Singh, Seema; Varman, Arul Mozhy

The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).

View Patent

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: NA0003525

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Number(s):: 11,453,895

Application Number:: 16/838,340

OSTI ID:: 1925142

Resource Relation:: Patent File Date: 04/02/2020

Country of Publication:: United States

Language:: English

References (53)

Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals Wu, Weihua; Dutta, Tanmoy; Varman, Arul M. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-07895-1	journal	August 2017
Lignin peroxidase functionalities and prospective applications Falade, Ayodeji O.; Nwodo, Uchechukwu U.; Iweriebor, Benson C. MicrobiologyOpen, Vol. 6, Issue 1 https://doi.org/10.1002/mbo3.394	journal	September 2016
Antioxidant activity applying an improved ABTS radical cation decolorization assay Re, Roberta; Pellegrini, Nicoletta; Proteggente, Anna Free Radical Biology and Medicine, Vol. 26, Issue 9-10 https://doi.org/10.1016/S0891-5849(98)00315-3	journal	May 1999
Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase Larsson, S.; Cassland, P.; Jonsson, L. J. Applied and Environmental Microbiology, Vol. 67, Issue 3, p. 1163-1170 https://doi.org/10.1128/AEM.67.3.1163-1170.2001	journal	March 2001
Recombinant Laccase: I. Enzyme cloning and characterization Nicolini, Claudio; Bruzzese, Debora; Cambria, Maria Teresa Journal of Cellular Biochemistry, Vol. 114, Issue 3 https://doi.org/10.1002/jcb.24397	journal	January 2013
Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences Barber-Zucker, Shiran; Mindel, Vladimir; Garcia-Ruiz, Eva Journal of the American Chemical Society, Vol. 144, Issue 8 https://doi.org/10.1021/jacs.1c12433	journal	February 2022
Engineering the ligninolytic enzyme consortium Alcalde, Miguel Trends in Biotechnology, Vol. 33, Issue 3 https://doi.org/10.1016/j.tibtech.2014.12.007	journal	March 2015
Comparison of Fungal Laccases and Redox Mediators in Oxidation of a Nonphenolic Lignin Model Compound Li, Kaichang; Xu, Feng; Eriksson, Karl-Erik L. Applied and Environmental Microbiology, Vol. 65, Issue 6 https://doi.org/10.1128/AEM.65.6.2654-2660.1999	journal	June 1999
Factors Influencing the Antioxidant Activity Determined by the ABTS^•+Radical Cation Assay Miller, Nicholas J.; Rice-Evans, Catherine A. Free Radical Research, Vol. 26, Issue 3 https://doi.org/10.3109/10715769709097799	journal	January 1997
Impact and prospective of fungal pre-treatment of lignocellulosic biomass for enzymatic hydrolysis Tian, Xiao-fei; Fang, Zhen; Guo, Feng Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 3 https://doi.org/10.1002/bbb.346	journal	January 2012
Structural Determinants of Oxidative Stabilization in an Evolved Versatile Peroxidase Gonzalez-Perez, David; Garcia-Ruiz, Eva; Ruiz-Dueñas, Francisco Javier ACS Catalysis, Vol. 4, Issue 11 https://doi.org/10.1021/cs501218v	journal	October 2014
Can laccases catalyze bond cleavage in lignin? Munk, Line; Sitarz, Anna K.; Kalyani, Dayanand C. Biotechnology Advances, Vol. 33, Issue 1 https://doi.org/10.1016/j.biotechadv.2014.12.008	journal	January 2015
Laccase engineering: From rational design to directed evolution Mate, Diana M.; Alcalde, Miguel Biotechnology Advances, Vol. 33, Issue 1 https://doi.org/10.1016/j.biotechadv.2014.12.007	journal	January 2015
Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria Brockmeier, Ulf; Caspers, Michael; Freudl, Roland Journal of Molecular Biology, Vol. 362, Issue 3 https://doi.org/10.1016/j.jmb.2006.07.034	journal	September 2006
Laccases and Their Applications: A Patent Review Kunamneni, Adinarayana; Plou, Francisco; Ballesteros, Antonio Recent Patents on Biotechnology, Vol. 2, Issue 1 https://doi.org/10.2174/187220808783330965	journal	January 2008
Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency Davis, Ryan Wesley; Singh, Seema; Wu, Huawen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8481974 US Patent Document 8,481,974	patent	July 2013
Lignin-degrading enzymes Pollegioni, Loredano; Tonin, Fabio; Rosini, Elena FEBS Journal, Vol. 282, Issue 7 https://doi.org/10.1111/febs.13224	journal	February 2015
Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature Cassland, P.; Jönsson, L. J. Applied Microbiology and Biotechnology, Vol. 52, Issue 3 https://doi.org/10.1007/s002530051537	journal	September 1999
Effect of pH on the stability of Pleurotus eryngii versatile peroxidase during heterologous production in Emericella nidulans L�-Chau, T. A.; Ruiz-Due�as, F. J.; Camarero, S. Bioprocess and Biosystems Engineering, Vol. 26, Issue 5 https://doi.org/10.1007/s00449-004-0365-1	journal	August 2004
Microbial Laccases and their Applications: A Review Desai, S. S.; Nityanand, C. Asian Journal of Biotechnology, Vol. 3, Issue 2 https://doi.org/10.3923/ajbkr.2011.98.124	journal	February 2011
Preparation of hydrophobic coatings Branson, Eric D.; Shah, Pratik; Singh, Seema https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7485343 US Patent Document 7,485,343	patent	February 2009
Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution Janusz, Grzegorz; Pawlik, Anna; Sulej, Justyna FEMS Microbiology Reviews, Vol. 41, Issue 6 https://doi.org/10.1093/femsre/fux049	journal	October 2017
Complete Sequence of the First Chimera Genome Constructed by Cloning the Whole Genome of Synechocystis Strain PCC6803 into the Bacillus subtilis 168 Genome Watanabe, Satoru; Shiwa, Yuh; Itaya, Mitsuhiro Journal of Bacteriology, Vol. 194, Issue 24 https://doi.org/10.1128/JB.01798-12	journal	December 2012
Laccase Variants Having Increased Expression and/or Activity Wang, Huaming; Ward, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140123404 US Patent Application 14/110123; 20140123404	patent-application	May 2014
Synthesis of novel ionic liquids from lignin-derived compounds Socha, Aaron; Singh, Seema; Simmons, Blake A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9765044 US Patent Document 9,765,044	patent	September 2017
Laboratory Evolution of High-Redox Potential Laccases Maté, Diana; García-Burgos, Carlos; García-Ruiz, Eva Chemistry & Biology, Vol. 17, Issue 9 https://doi.org/10.1016/j.chembiol.2010.07.010	journal	September 2010
Structural Characterization of Lignin and Its Degradation Products with Spectroscopic Methods Lu, Yao; Lu, Yong-Chao; Hu, Hong-Qin Journal of Spectroscopy, Vol. 2017 https://doi.org/10.1155/2017/8951658	journal	January 2017
Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli Phan, Trang Thi Phuong; Tran, Linh Thuoc; Schumann, Wolfgang Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0255-z	journal	May 2015
Enzymatic lignocellulose hydrolysis: Improved cellulase productivity by insoluble solids recycling Weiss, Noah; Börjesson, Johan; Pedersen, Lars Saaby Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-5	journal	January 2013
Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements Phan, Trang Thi Phuong; Nguyen, Hoang Duc; Schumann, Wolfgang Journal of Biotechnology, Vol. 157, Issue 1 https://doi.org/10.1016/j.jbiotec.2011.10.006	journal	January 2012
Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus Camarero, S.; Pardo, I.; Cañas, A. I. Applied and Environmental Microbiology, Vol. 78, Issue 5 https://doi.org/10.1128/AEM.07530-11	journal	December 2011
The making of versatile peroxidase by directed evolution Gonzalez-Perez, David; Alcalde, Miguel Biocatalysis and Biotransformation, Vol. 36, Issue 1 https://doi.org/10.1080/10242422.2017.1363190	journal	August 2017
Development of chimeric laccases by directed evolution Pardo, Isabel; Vicente, Ana Isabel; Mate, Diana M. Biotechnology and Bioengineering, Vol. 109, Issue 12 https://doi.org/10.1002/bit.24588	journal	July 2012
Toward engineering E. coli with an autoregulatory system for lignin valorization Wu, Weihua; Liu, Fang; Singh, Seema Proceedings of the National Academy of Sciences, Vol. 115, Issue 12 https://doi.org/10.1073/pnas.1720129115	journal	March 2018
Improvement of hydrogen peroxide stability of Pleurotus eryngii versatile ligninolytic peroxidase by rational protein engineering Bao, Xue; Huang, Xuenian; Lu, Xuefeng Enzyme and Microbial Technology, Vol. 54 https://doi.org/10.1016/j.enzmictec.2013.10.003	journal	January 2014
Assembly of evolved ligninolytic genes inSaccharomyces cerevisiae Gonzalez-Perez, David; Alcalde, Miguel Bioengineered, Vol. 5, Issue 4 https://doi.org/10.4161/bioe.29167	journal	May 2014
Lignin biodegradation and industrial implications B. Fisher, Adam; S. Fong, Stephen AIMS Bioengineering, Vol. 1, Issue 2 https://doi.org/10.3934/bioeng.2014.2.92	journal	January 2014
Synthesis of novel ionic liquids from lignin-derived compounds Socha, Aaron; Singh, Seema; Simmons, Blake A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10155735 US Patent Document 10,155,735	patent	December 2018
Laccase: a multi‐purpose biocatalyst at the forefront of biotechnology Mate, Diana M.; Alcalde, Miguel Microbial Biotechnology, Vol. 10, Issue 6 https://doi.org/10.1111/1751-7915.12422	journal	October 2016
Renewable aromatics from lignocellulosic lignin Singh, Seema; Varanasi, Patanjali; Simmons, Blake A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10208076 US Patent Document 10,208,076	patent	February 2019
Blood Tolerant Laccase by Directed Evolution Mate, Diana M.; Gonzalez-Perez, David; Falk, Magnus Chemistry & Biology, Vol. 20, Issue 2 https://doi.org/10.1016/j.chembiol.2013.01.001	journal	February 2013
Decoding how a soil bacterium extracts building blocks and metabolic energy from ligninolysis provides road map for lignin valorization Varman, Arul M.; He, Lian; Follenfant, Rhiannon Proceedings of the National Academy of Sciences, Vol. 113, Issue 40 https://doi.org/10.1073/pnas.1606043113	journal	September 2016
Multiplexed screening of enzyme activities using nanostructure-initiator mass spectrometry Northen, Trent R.; Reindl, Wolfgang E.; Deng, Kai https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10240180 US Patent Document 10,240,180	patent	March 2019
Plant cell wall lignification and monolignol metabolism Wang, Yin; Chantreau, Maxime; Sibout, Richard Frontiers in Plant Science, Vol. 4 https://doi.org/10.3389/fpls.2013.00220	journal	January 2013
Protein tolerance to random amino acid change Guo, H. H.; Choe, J.; Loeb, L. A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210 https://doi.org/10.1073/pnas.0403255101	journal	June 2004
Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay Müller, Lars; Fröhlich, Kati; Böhm, Volker Food Chemistry, Vol. 129, Issue 1 https://doi.org/10.1016/j.foodchem.2011.04.045	journal	November 2011
Renewable aromatics from lignocellulosic lignin Singh, Seema; Varanasi, Patanjali; Simmons, Blake A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10233292 US Patent Document 10,233,292	patent	March 2019
Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes Gonzalez-Perez, David; Garcia-Ruiz, Eva; Alcalde, Miguel Bioengineered, Vol. 3, Issue 3 https://doi.org/10.4161/bbug.19544	journal	May 2012
Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review Fillat, Úrsula; Ibarra, David; Eugenio, María Fermentation, Vol. 3, Issue 2 https://doi.org/10.3390/fermentation3020017	journal	May 2017
Alkaline versatile peroxidase by directed evolution Gonzalez-Perez, David; Mateljak, Ivan; Garcia-Ruiz, Eva Catalysis Science & Technology, Vol. 6, Issue 17 https://doi.org/10.1039/C6CY01044J	journal	January 2016
HMF production from glucose in ionic liquid media Sathitsuksanoh, Noppadon; Travisano, Philip; George, Anthe https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10112916 US Patent Document 10,112,916	patent	October 2018
OPTIMIZER: a web server for optimizing the codon usage of DNA sequences Puigbo, P.; Guzman, E.; Romeu, A. Nucleic Acids Research, Vol. 35, Issue Web Server https://doi.org/10.1093/nar/gkm219	journal	May 2007
Engineering and Applications of fungal laccases for organic synthesis Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos Microbial Cell Factories, Vol. 7, Issue 1 https://doi.org/10.1186/1475-2859-7-32	journal	January 2008

Similar Records

Defining the Minimal Set of Microbial Genes Required for Valorization of Lignin Biomass (Final Report)

Technical Report · Fri Sep 22 00:00:00 EDT 2023 · OSTI ID:1925142

Sattely, Elizabeth; Khlystov, Nikita

Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes

Journal Article · Thu Jan 01 00:00:00 EST 2009 · Biotechnology for Biofuels · OSTI ID:1925142

Tartar, Aurélien; Wheeler, Marsha M.; Zhou, Xuguo; +3 more

Enzymes that cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides

Patent · Tue Oct 24 00:00:00 EDT 2017 · OSTI ID:1925142

Kravit, Nancy G.; Schmidt, Katherine A.

Title: Engineered hosts with exogenous ligninase and uses thereof

Citation Formats

References (53)

Similar Records

Related Subjects