Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger

Gladden, John M.; Campen, Saori Amaike; Zhang, Jinxiang; Magnuson, Jon K.; Baker, Scott E.; Simmons, Blake A.

Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger

Patent · Tue Aug 16 00:00:00 EDT 2022

OSTI ID:1924985

Gladden, John M.; Campen, Saori Amaike; Zhang, Jinxiang; Magnuson, Jon K.; Baker, Scott E.; Simmons, Blake A.

The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

View Patent

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM); Battelle Memorial Institute (Richland, WA); The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,414,653

Application Number:: 16/124,127

OSTI ID:: 1924985

Country of Publication:: United States

Language:: English

References (48)

Fusion PCR and gene targeting in Aspergillus nidulans Szewczyk, Edyta; Nayak, Tania; Oakley, C. Elizabeth Nature Protocols, Vol. 1, Issue 6 https://doi.org/10.1038/nprot.2006.405	journal	December 2006
Regulation of the glaA gene of Aspergillus niger Fowler, Timothy; Berka, Randy M.; Ward, Michael Current Genetics, Vol. 18, Issue 6 https://doi.org/10.1007/BF00327025	journal	January 1990
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Livak, Kenneth J.; Schmittgen, Thomas D. Methods, Vol. 25, Issue 4 https://doi.org/10.1006/meth.2001.1262	journal	December 2001
Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast Zhang, Jin; Astorga, Maria A.; Gardner, Jennifer M. Metabolic Engineering, Vol. 45 https://doi.org/10.1016/j.ymben.2017.12.012	journal	January 2018
Comparative genomics and transcriptome analysis of Aspergillus niger and metabolic engineering for citrate production Yin, Xian; Shin, Hyun-dong; Li, Jianghua Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep41040	journal	January 2017
Characterization of a polyketide synthase in Aspergillus niger whose product is a precursor for both dihydroxynaphthalene (DHN) melanin and naphtho-γ-pyrone Chiang, Yi-Ming; Meyer, Kristen M.; Praseuth, Michael Fungal Genetics and Biology, Vol. 48, Issue 4 https://doi.org/10.1016/j.fgb.2010.12.001	journal	April 2011
The Cell Surface Protein Geneecm33⁺Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast Takada, Hirofumi; Nishida, Aiko; Domae, Mitsuhiro Molecular Biology of the Cell, Vol. 21, Issue 4 https://doi.org/10.1091/mbc.e09-09-0810	journal	February 2010
Discovery and characterization of ionic liquid-tolerant thermophilic cellulases from a switchgrass-adapted microbial community Gladden, John M.; Park, Joshua I.; Bergmann, Jessica Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-15	journal	January 2014
Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli Punt, Peter J.; Oliver, Richard P.; Dingemanse, Maria A. Gene, Vol. 56, Issue 1 https://doi.org/10.1016/0378-1119(87)90164-8	journal	January 1987
Filamentous fungi as cell factories for heterologous protein production Punt, Peter J.; van Biezen, Nick; Conesa, Ana Trends in Biotechnology, Vol. 20, Issue 5, p. 200-206 https://doi.org/10.1016/S0167-7799(02)01933-9	journal	May 2002
A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels Park, Joshua I.; Steen, Eric J.; Burd, Helcio PLoS ONE, Vol. 7, Issue 5, Article No. e37010 https://doi.org/10.1371/journal.pone.0037010	journal	May 2012
Specific expression and temperature-dependent expression of the acid protease-encoding gene (pepA) in Aspergillus oryzae in solid-state culture (Rice-Koji) Kitano, Hideyuki; Kataoka, Kouhei; Furukawa, Keiji Journal of Bioscience and Bioengineering, Vol. 93, Issue 6 https://doi.org/10.1016/S1389-1723(02)80238-9	journal	January 2002
ACEI of Trichoderma reesei Is a Repressor of Cellulase and Xylanase Expression Aro, N.; Ilmen, M.; Saloheimo, A. Applied and Environmental Microbiology, Vol. 69, Issue 1 https://doi.org/10.1128/AEM.69.1.56-65.2003	journal	January 2003
Chapter One - Heterologous Gene Expression in Filamentous Fungi Su, Xiaoyun; Schmitz, George; Zhang, Meiling Advances in Applied Microbiology, Vol. 81, p. 1-61 https://doi.org/10.1016/B978-0-12-394382-8.00001-0	book	January 2012
The Generally Recognized as Safe (GRAS) Process for Industrial Microbial Enzymes Sewalt, Vincent; Shanahan, Diane; Gregg, Lori Industrial Biotechnology, Vol. 12, Issue 5 https://doi.org/10.1089/ind.2016.0011	journal	October 2016
Detection of a protein, AngCP, which binds specifically to the three upstream regions of glaA gene in A. niger T21 Qiu, Runxiang Science in China Series C, Vol. 45, Issue 5 https://doi.org/10.1360/02yc9058	journal	January 2002
An upstream activating sequence from the Aspergillus nidulans gpdA gene Punt, Peter J.; Kramer, Cas; Kuyvenhoven, Anneke Gene, Vol. 120, Issue 1 https://doi.org/10.1016/0378-1119(92)90010-M	journal	October 1992
Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase Punt, Peter J.; Dingemanse, Maria A.; Kuyvenhoven, Anneke Gene, Vol. 93, Issue 1 https://doi.org/10.1016/0378-1119(90)90142-E	journal	September 1990
Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon cataboiite repression in Aspergillus nidulans Kulmburg, P.; Mathieu, M.; Dowzer, C. Molecular Microbiology, Vol. 7, Issue 6 https://doi.org/10.1111/j.1365-2958.1993.tb01175.x	journal	March 1993
Developing Aspergillus as a host for heterologous expression Lubertozzi, David; Keasling, Jay D. Biotechnology Advances, Vol. 27, Issue 1 https://doi.org/10.1016/j.biotechadv.2008.09.001	journal	January 2009
Transformation of Aspergillus nidulans by using a trpC plasmid. Yelton, M. M.; Hamer, J. E.; Timberlake, W. E. Proceedings of the National Academy of Sciences, Vol. 81, Issue 5, p. 1470-1474 https://doi.org/10.1073/pnas.81.5.1470	journal	March 1984
One Single Basic Amino Acid at the ω-1 or ω-2 Site Is a Signal That Retains Glycosylphosphatidylinositol-Anchored Protein in the Plasma Membrane of Aspergillus fumigatus Ouyang, Haomiao; Chen, Xiaomin; Lü, Yang Eukaryotic Cell, Vol. 12, Issue 6 https://doi.org/10.1128/EC.00351-12	journal	June 2013
5′ Untranslated region of the Hsp12 gene contributes to efficient translation in Aspergillus oryzae Koda, Akio; Bogaki, Takayuki; Minetoki, Toshitaka Applied Microbiology and Biotechnology, Vol. 70, Issue 3 https://doi.org/10.1007/s00253-005-0083-x	journal	April 2006
Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins Champer, Jackson; Ito, James; Clemons, Karl Journal of Fungi, Vol. 2, Issue 1 https://doi.org/10.3390/jof2010006	journal	January 2016
Production of first and second generation biofuels: A comprehensive review Naik, S. N.; Goud, Vaibhav V.; Rout, Prasant K. Renewable and Sustainable Energy Reviews, Vol. 14, Issue 2 https://doi.org/10.1016/j.rser.2009.10.003	journal	February 2010
Translation efficiency mediated by the 5? untranslated region greatly affects protein production in Aspergillus oryzae Koda, Akio; Minetoki, Toshitaka; Ozeki, Kenji Applied Microbiology and Biotechnology, Vol. 66, Issue 3 https://doi.org/10.1007/s00253-004-1681-8	journal	August 2004
A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors Colot, H. V.; Park, G.; Turner, G. E. Proceedings of the National Academy of Sciences, Vol. 103, Issue 27 https://doi.org/10.1073/pnas.0601456103	journal	June 2006
On the safety of Aspergillus niger - a review Schuster, E.; Dunn-Coleman, N.; Frisvad, J. Applied Microbiology and Biotechnology, Vol. 59, Issue 4-5, p. 426-435 https://doi.org/10.1007/s00253-002-1032-6	journal	January 2002
Aspergillus niger genomics: Past, present and into the future Baker, Scott E. Medical Mycology, Vol. 44, Issue s1 https://doi.org/10.1080/13693780600921037	journal	January 2006
Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice McCutcheon, Susan C.; Jones, Ken; Cumming, Sarah A. BMC Biology, Vol. 8, Issue 1 https://doi.org/10.1186/1741-7007-8-89	journal	June 2010
Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling Liu, Gang; Zhang, Jian; Bao, Jie Bioprocess and Biosystems Engineering, Vol. 39, Issue 1 https://doi.org/10.1007/s00449-015-1497-1	journal	November 2015
PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity Pardo, M. Microbiology, Vol. 150, Issue 12 https://doi.org/10.1099/mic.0.26924-0	journal	December 2004
Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88 Andersen, M. R.; Salazar, M. P.; Schaap, P. J. Genome Research, Vol. 21, Issue 6, p. 885-897 https://doi.org/10.1101/gr.112169.110	journal	May 2011
Characterization of Humanized Antibodies Secreted by Aspergillus niger Ward, M.; Lin, C.; Victoria, D. C. Applied and Environmental Microbiology, Vol. 70, Issue 5 https://doi.org/10.1128/AEM.70.5.2567-2576.2004	journal	May 2004
Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae amylase-encoding genes Minetoki, T.; Kumagai, C.; Gomi, K. Applied Microbiology and Biotechnology, Vol. 50, Issue 4 https://doi.org/10.1007/s002530051321	journal	October 1998
Global Proteomic Profiling of the Secretome of Candida albicans ecm33 Cell Wall Mutant Reveals the Involvement of Ecm33 in Sap2 Secretion Gil-Bona, Ana; Monteoliva, Lucía; Gil, Concha Journal of Proteome Research, Vol. 14, Issue 10 https://doi.org/10.1021/acs.jproteome.5b00411	journal	August 2015
Proteomic Analysis of the Secretory Response of Aspergillus niger to D-Maltose and D-Xylose Ferreira de Oliveira, José Miguel P.; van Passel, Mark W. J.; Schaap, Peter J. PLoS ONE, Vol. 6, Issue 6 https://doi.org/10.1371/journal.pone.0020865	journal	June 2011
E3 Ubiquitin Ligase Pub1 Is implicated in Endocytosis of a GPI-Anchored Protein Ecm33 in Fission Yeast Fang, Yue; Jaiseng, Wurentuya; Ma, Yan PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0085238	journal	January 2014
The challenge of enzyme cost in the production of lignocellulosic biofuels Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A. Biotechnology and Bioengineering, Vol. 109, Issue 4 https://doi.org/10.1002/bit.24370	journal	November 2011
The Cell Wall Protein Ecm33 of Candida albicans is Involved in Chronological Life Span, Morphogenesis, Cell Wall Regeneration, Stress Tolerance, and Host–Cell Interaction Gil-Bona, Ana; Reales-Calderon, Jose A.; Parra-Giraldo, Claudia M. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00064	journal	February 2016
A new transcriptional activator for amylase genes in Aspergillus Petersen, K. L.; Lehmbeck, J.; Christensen, T. Molecular and General Genetics MGG, Vol. 262, Issue 4-5 https://doi.org/10.1007/s004380051129	journal	December 1999
Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger Amaike Campen, Saori; Lynn, Jed; Sibert, Stephanie J. PLOS ONE, Vol. 12, Issue 12 https://doi.org/10.1371/journal.pone.0189604	journal	December 2017
Disruption of the Aspergillus fumigatus ECM33 homologue results in rapid conidial germination, antifungal resistance and hypervirulence Romano, Jacob; Nimrod, Guy; Ben-Tal, Nir Microbiology, Vol. 152, Issue 7 https://doi.org/10.1099/mic.0.28936-0	journal	July 2006
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Bok, Jin Woo; Keller, Nancy P. Methods in Molecular Biology https://doi.org/10.1007/978-1-62703-122-6_11	book	January 2012
An expression system based on the promoter region of the Aspergillus awamori 1,4-β-endoxylanase A gene Gouka, R. J.; Hessing, J. G. M.; Punt, P. J. Applied Microbiology and Biotechnology, Vol. 46, Issue 1 https://doi.org/10.1007/s002530050779	journal	August 1996
Proteogenomic Analysis of a Thermophilic Bacterial Consortium Adapted to Deconstruct Switchgrass D'haeseleer, Patrik; Gladden, John M.; Allgaier, Martin PLoS ONE, Vol. 8, Issue 7, Article No. e68465 https://doi.org/10.1371/journal.pone.0068465	journal	July 2013
Isolation of the ace1 Gene Encoding a Cys2-His2 Transcription Factor Involved in Regulation of Activity of the Cellulase Promoter cbh1of Trichoderma reesei Saloheimo, Anu; Aro, Nina; Ilmén, Marja Journal of Biological Chemistry, Vol. 275, Issue 8 https://doi.org/10.1074/jbc.275.8.5817	journal	February 2000
The GPI-anchored protein CaEcm33p is required for cell wall integrity, morphogenesis and virulence in Candida albicans Martinez-Lopez, R. Microbiology, Vol. 150, Issue 10 https://doi.org/10.1099/mic.0.27320-0	journal	October 2004

Similar Records

Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger

Book · Fri Jun 01 00:00:00 EDT 2018 · OSTI ID:1768010

Production of extremophilic bacterial cellulase enzymes in aspergillus niger.

Technical Report · Sun Sep 01 00:00:00 EDT 2013 · OSTI ID:1096445

Analytical and computational approaches to define the Aspergillus niger secretome

Journal Article · Sat Feb 28 23:00:00 EST 2009 · Fungal Genetics and Biology : FG & B, 46(1, Suppl. 1):S153-S160 · OSTI ID:979507

Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger

Citation Formats

References (48)

Similar Records

Related Subjects