A porcine ligated loop model reveals new insight into the host immune response against Campylobacter jejuni

Negretti, Nicholas M.; Ye, Yinyin; Malavasi, Lais M.; Pokharel, Swechha M.; Huynh, Steven; Noh, Susan; Klima, Cassidy; Gourley, Christopher R.; Ragle, Claude A.; Bose, Santanu; Looft, Torey; Parker, Craig T.; Clair, Geremy CD; Adkins, Joshua N.; Konkel, Michael

doi:10.1080/19490976.2020.1814121

A porcine ligated loop model reveals new insight into the host immune response against Campylobacter jejuni

Journal Article · Fri Sep 11 04:00:00 EDT 2020 · Gut Microbes

DOI:https://doi.org/10.1080/19490976.2020.1814121· OSTI ID:1924514

Negretti, Nicholas M. ^[1]; Ye, Yinyin ^[2]; Malavasi, Lais M. ^[1]; Pokharel, Swechha M. ^[1]; Huynh, Steven ^[3]; Noh, Susan ^[1]; Klima, Cassidy ^[4]; Gourley, Christopher R. ^[1]; Ragle, Claude A. ^[1]; Bose, Santanu ^[1]; Looft, Torey ^[5]; Parker, Craig T. ^[6]; ^[2]; ^[2]; Konkel, Michael ^[1]

Washington State University
BATTELLE (PACIFIC NW LAB)
United States Department of Agriculture-Agricultural Research Service
US Department of Agriculture
Iowa State University
USDA-ARS Western Regional Research Center

The symptoms of infectious diarrheal disease are mediated by a combination of a pathogen’s virulence factors and the host immune system. Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide due to its near-ubiquitous zoonotic association with poultry. One of the outstanding questions is to what extent the bacteria are responsible for the diarrheal symptoms via intestinal cell necrosis versus immune cell initiated tissue damage. To determine the stepwise process of inflammation that leads to diarrhea, we used a piglet ligated intestinal loop model to study the intestinal response to C. jejuni. Pigs were chosen due to the anatomical similarity between the porcine and the human intestine. We found that the abundance of neutrophil related proteins increased in the intestinal lumen during C. jejuni infection, including proteins related to neutrophil migration (neutrophil elastase and MMP9), actin reorganization (Arp2/3), and antimicrobial proteins (lipocalin-2, myeloperoxidase, S100A8, and S100A9). The appearance of neutrophil proteins also corresponded with increases of the inflammatory cytokines IL-8 and TNF-a. Compared to infection with the C. jejuni wild-type strain, infection with the noninvasive C. jejuni ?ciaD mutant resulted in a blunted inflammatory response, with less inflammatory cytokines and neutrophil markers. These findings indicate that intestinal inflammation is driven by C. jejuni virulence and that neutrophils are the predominant cell type responding to C. jejuni infection. We propose that this model can be used as a platform to study the early immune events during infection with intestinal pathogens.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1924514

Report Number(s):: PNNL-SA-166370

Journal Information:: Gut Microbes, Journal Name: Gut Microbes Journal Issue: 1 Vol. 12

Country of Publication:: United States

Language:: English

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A porcine ligated loop model reveals new insight into the host immune response against Campylobacter jejuni

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A porcine ligated loop model reveals new insight into the host immune response against Campylobacter jejuni

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