skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Resolution of thylakoid polyphenol oxidase and a protein kinase

Abstract

The predominant protein kinase activity in octylglucoside (OG) extracts of spinach thylakoids has been attributed to a 64-kDa protein, tp64. Recent work calls into question the relation between tp64 and protein kinase activity, which were fractionated apart using fluid phase IEF and hydroxylapatite chromatography. Hind et al. sequenced tp64 from the cDNA and showed it to be a polyphenol oxidase (PPO) homolog. Its transit peptide indicates a location for the mature protein within the thylakoid lumen, where there is presumably no ATP and where it is remote from the presumed kinase substrates: the stromally exposed regions of integral PS-II membrane proteins. Here the authors suggest that the kinase is a 64-kDa protein distinct from tp64.

Authors:
; ;
Publication Date:
Research Org.:
Brookhaven National Lab., Upton, NY (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
192405
Report Number(s):
BNL-62487; CONF-9508202-3
ON: DE96003576; TRN: 96:001567
DOE Contract Number:
AC02-76CH00016
Resource Type:
Conference
Resource Relation:
Conference: 10. international photosynthesis congress, Montpellier (France), 20-25 Aug 1995; Other Information: PBD: 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; THYLAKOID MEMBRANE PROTEINS; BIOCHEMISTRY

Citation Formats

Race, H.L., Davenport, J.W., and Hind, G. Resolution of thylakoid polyphenol oxidase and a protein kinase. United States: N. p., 1995. Web.
Race, H.L., Davenport, J.W., & Hind, G. Resolution of thylakoid polyphenol oxidase and a protein kinase. United States.
Race, H.L., Davenport, J.W., and Hind, G. Sun . "Resolution of thylakoid polyphenol oxidase and a protein kinase". United States. doi:. https://www.osti.gov/servlets/purl/192405.
@article{osti_192405,
title = {Resolution of thylakoid polyphenol oxidase and a protein kinase},
author = {Race, H.L. and Davenport, J.W. and Hind, G.},
abstractNote = {The predominant protein kinase activity in octylglucoside (OG) extracts of spinach thylakoids has been attributed to a 64-kDa protein, tp64. Recent work calls into question the relation between tp64 and protein kinase activity, which were fractionated apart using fluid phase IEF and hydroxylapatite chromatography. Hind et al. sequenced tp64 from the cDNA and showed it to be a polyphenol oxidase (PPO) homolog. Its transit peptide indicates a location for the mature protein within the thylakoid lumen, where there is presumably no ATP and where it is remote from the presumed kinase substrates: the stromally exposed regions of integral PS-II membrane proteins. Here the authors suggest that the kinase is a 64-kDa protein distinct from tp64.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 31 00:00:00 EST 1995},
month = {Sun Dec 31 00:00:00 EST 1995}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: