A newly isolated alkaliphilic cyanobacterium for biomass production with direct air CO2 capture

Gao, Song; Pittman, Kyle; Edmundson, Scott; Huesemann, Michael; Greer, Mattias; Louie, William; Chen, Peter; Nobles, David; Benemann, John; Crowe, Braden

doi:10.1016/j.jcou.2023.102399

Title: A newly isolated alkaliphilic cyanobacterium for biomass production with direct air CO2 capture

Journal Article · Wed Mar 01 00:00:00 EST 2023 · Journal of CO2 Utilization

DOI:https://doi.org/10.1016/j.jcou.2023.102399· OSTI ID:1923589

; Pittman, Kyle;

; Huesemann, Michael; Greer, Mattias; Louie, William; Chen, Peter;

; Benemann, John;

Large-scale microalgal biomass production usually relies on concentrated CO₂ as the carbon source which is a major logistical and economic challenge because of the required infrastructure and geospatial constraints of siting large algal production facilities close to concentrated CO₂ sources. By taking advantage of enhanced CO₂ mass transfer within alkaline media due to the reaction between CO₂ and OH^-, carbon can be directly captured from the ambient air and used by the algae. Achieving such goal requires algal strains that can grow fast under alkaline conditions. In this study, a newly isolated alkaliphilic cyanobacterial strain Cyanobacterium sp. PNNL-SSL1 was characterized for its temperature, pH, and salinity tolerance. The result shows that PNNL-SSL1 could sustain grow at pH above 11.2 but exhibited the highest growth rates at pH below 10.5. The strain is a warm season strain and grows the best at 10 PSU (practical salinity unit). The strain was then evaluated in indoor climate simulation ponds under outdoor-relevant pond cultivation conditions with carbon supplied only through direct air CO₂ capture at the culture surface. Two batch culture runs, at slightly different initial pH, exhibited average biomass productivity of 15.2 g m^-2 day^-1 on ash-free dry weight basis. CO₂ was directly captured from the air at an average rate of 21.6 g CO₂ m^-2 day^-1 (78.8 tons ha^-1 yr^-1), contributing 74% of the carbon fixed in the biomass. The results demonstrated that PNNL-SSL1 is a promising strain for biomass production using air CO₂ as the carbon source.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: EE0007004; AC05-76RL01830

OSTI ID:: 1923589

Alternate ID(s):: OSTI ID: 1924496

Report Number(s):: PNNL-SA-178327; S2212982023000100; 102399; PII: S2212982023000100

Journal Information:: Journal of CO2 Utilization, Journal Name: Journal of CO2 Utilization Vol. 69 Journal Issue: C; ISSN 2212-9820

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Alkaliphilic cyanobacteria
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Direct air capture

Title: A newly isolated alkaliphilic cyanobacterium for biomass production with direct air CO2 capture

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