Nitrogen addition alters soil fungal communities, but root fungal communities are resistant to change

Carrell, Alyssa A.; Hicks, Brittany B.; Sidelinger, Emilie; Johnston, Eric R.; Jawdy, Sara S.; Clark, Miranda M.; Klingeman, Dawn M.; Cregger, Melissa A.

doi:10.3389/fmicb.2022.1033631

Nitrogen addition alters soil fungal communities, but root fungal communities are resistant to change

Journal Article · Wed Jan 25 00:00:00 EST 2023 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2022.1033631· OSTI ID:1923176

Carrell, Alyssa A. ^[1]; Hicks, Brittany B. ^[2]; Sidelinger, Emilie ^[3]; ^[1]; Jawdy, Sara S. ^[1]; ^[1]; ^[1]; ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Michigan, Ann Arbor, MI (United States)
Clemson Univ., SC (United States)

Plants are colonized by numerous microorganisms serving important symbiotic functions that are vital to plant growth and success. Understanding and harnessing these interactions will be useful in both managed and natural ecosystems faced with global change, but it is still unclear how variation in environmental conditions and soils influence the trajectory of these interactions. In this study, we examine how nitrogen addition alters plant-fungal interactions within two species of Populus - Populus deltoides and P. trichocarpa. In this experiment, we manipulated plant host, starting soil (native vs. away for each tree species), and nitrogen addition in a fully factorial replicated design. After ~10 weeks of growth, we destructively harvested the plants and characterized plant growth factors and the soil and root endosphere fungal communities using targeted amplicon sequencing of the ITS2 gene region. Overall, we found nitrogen addition altered plant growth factors, e.g., plant height, chlorophyll density, and plant N content. Interestingly, nitrogen addition resulted in a lower fungal alpha diversity in soils but not plant roots. Further, there was an interactive effect of tree species, soil origin, and nitrogen addition on soil fungal community composition. Starting soils collected from Oregon and West Virginia were dominated by the ectomycorrhizal fungi Inocybe (55.8% relative abundance), but interestingly when P. deltoides was grown in its native West Virginia soil, the roots selected for a high abundance of the arbuscular mycorrhizal fungi, Rhizophagus. These results highlight the importance of soil origin and plant species on establishing plant-fungal interactions.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1923176

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Journal Issue: 1 Vol. 13; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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References (54)

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