Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes

Du, Juan; Vandavasi, Venu Gopal; Molloy, Kelly R.; Yang, Hui; Massenburg, Lynnicia N.; Singh, Abhishek; Kwansa, Albert L.; Yingling, Yaroslava G.; O’Neill, Hugh; Chait, Brian T.; Kumar, Manish; Nixon, B. Tracy

doi:10.1021/acs.biomac.2c00550

Title: Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes

Journal Article · Wed Aug 10 00:00:00 EDT 2022 · Biomacromolecules

DOI:https://doi.org/10.1021/acs.biomac.2c00550· OSTI ID:1922484

Du, Juan ^[1]; Vandavasi, Venu Gopal ^[2]; Molloy, Kelly R. ^[3];

^[4]; Massenburg, Lynnicia N. ^[4]; Singh, Abhishek ^[5]; Kwansa, Albert L. ^[5];

^[5];

^[2]; Chait, Brian T. ^[3];

^[4];

^[4]

Sichuan Univ., Chengdu (China); Pennsylvania State Univ., University Park, PA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Rockefeller Univ., New York, NY (United States)
Pennsylvania State Univ., University Park, PA (United States)
North Carolina State Univ., Raleigh, NC (United States)

Higher plants synthesize cellulose using membrane-bound, six-lobed cellulose synthase complexes, each lobe containing trimeric cellulose synthases (CESAs). Although molecular biology reports support heteromeric trimers composed of different isoforms, a homomeric trimer was reported for in vitro studies of the catalytic domain of CESA1 of Arabidopsis (AtCESA1CatD) and confirmed in cryoEM structures of full-length CESA8 and CESA7 of poplar and cotton, respectively. In both structures, a small portion of the plant-conserved region (P-CR) forms the only contacts between catalytic domains of the monomers. Here, we report inter-subunit lysine-crosslinks that localize to the small P-CR, negative-stain EM structure, and modeling data for homotrimers of AtCESA1CatD. Molecular dynamics simulations for AtCESA1CatD trimers based on the CESA8 cryoEM structure were stable and dependent upon a small set of residue contacts. The results suggest that homomeric CESA trimers may be important for the synthesis of primary and secondary cell walls and identify key residues for future mutagenic studies.

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Research Organization:: Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: SC0001090; P41 GM109824; P41 GM103314

OSTI ID:: 1922484

Journal Information:: Biomacromolecules, Vol. 23, Issue 9; ISSN 1525-7797

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Arabidopsis

Title: Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes

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