The Chlamydomonas Genome Project, version 6: reference assemblies for mating type plus and minus strains reveal extensive structural mutation in the laboratory

Craig, Rory J.; Gallaher, Sean D.; Shu, Shengqiang; Salomé, Patrice; Jenkins, Jerry W.; Blaby-Haas, Crysten E.; Purvine, Samuel O.; O’Donnell, Samuel; Barry, Kerrie; Grimwood, Jane; Strenkert, Daniela; Kropat, Janette; Daum, Chris; Yoshinaga, Yuko; Goodstein, David M.; Vallon, Olivier; Schmutz, Jeremy; Merchant, Sabeeha S.

doi:10.1093/plcell/koac347

The Chlamydomonas Genome Project, version 6: reference assemblies for mating type plus and minus strains reveal extensive structural mutation in the laboratory

Journal Article · Thu Dec 22 23:00:00 EST 2022 · The Plant Cell

DOI:https://doi.org/10.1093/plcell/koac347· OSTI ID:1909760

Craig, Rory J. ^[1]; Gallaher, Sean D. ^[2]; Shu, Shengqiang ^[3]; Salomé, Patrice ^[4]; Jenkins, Jerry W. ^[5]; Blaby-Haas, Crysten E. ^[6]; Purvine, Samuel O. ^[7]; O’Donnell, Samuel ^[8]; Barry, Kerrie ^[3]; Grimwood, Jane ^[5]; Strenkert, Daniela ^[2]; Kropat, Janette ^[4]; Daum, Chris ^[3]; Yoshinaga, Yuko ^[3]; Goodstein, David M. ^[3]; Vallon, Olivier ^[8]; Schmutz, Jeremy ^[9]; ^[10]

Univ. of California, Berkeley, CA (United States); Univ. of Edinburgh (United Kingdom); California Institute for Quantitative Biosciences, University of California, Berkeley
Univ. of California, Berkeley, CA (United States)
Joint Genome Inst., Berkeley, CA (United States)
Univ. of California, Los Angeles, CA (United States)
HudsonAlpha Inst. for Biotechnology, Huntsville, AL (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sorbonne Univ., Paris (France)
Joint Genome Inst., Berkeley, CA (United States); HudsonAlpha Inst. for Biotechnology, Huntsville, AL (United States)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Five versions of the Chlamydomonas reinhardtii reference genome have been produced over the last two decades. Here we present version 6, bringing significant advances in assembly quality and structural annotations. PacBio-based chromosome-level assemblies for two laboratory strains, CC-503 and CC-4532, provide resources for the plus and minus mating type alleles. We corrected major misassemblies in previous versions and validated our assemblies via linkage analyses. Contiguity increased over ten-fold and >80% of filled gaps are within genes. We used Iso-Seq and deep RNA-seq datasets to improve structural annotations, and updated gene symbols and textual annotation of functionally characterized genes via extensive manual curation. We discovered that the cell wall-less classical reference strain CC-503 exhibits genomic instability potentially caused by deletion of the helicase RECQ3, with major structural mutations identified that affect >100 genes. We therefore present the CC-4532 assembly as the primary reference, although this strain also carries unique structural mutations and is experiencing rapid proliferation of a Gypsy retrotransposon. We expect all laboratory strains to harbor gene-disrupting mutations, which should be considered when interpreting and comparing experimental results. Collectively, the resources presented here herald a new era of Chlamydomonas genomics and will provide the foundation for continued research in this important reference organism.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1909760

Alternate ID(s):: OSTI ID: 1958839

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 2 Vol. 35; ISSN 1040-4651

Publisher:: American Society of Plant Biologists (ASPB)Copyright Statement

Country of Publication:: United States

Language:: English

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