Recognition of glycoside hydrolase 12 proteins by the immune receptor RXEG1 confers Fusarium head blight resistance in wheat

Wang, Zongkuan; Yang, Bo; Zheng, Wenyue; Wang, Lei; Cai, Xingxing; Yang, Jie; Song, Rongrong; Yang, Sen; Wang, Yuyin; Xiao, Jin; Liu, Huiquan; Wang, Yan; Wang, Xiue; Wang, Yuanchao

doi:10.1111/pbi.13995

Recognition of glycoside hydrolase 12 proteins by the immune receptor RXEG1 confers Fusarium head blight resistance in wheat

Journal Article · Tue Jan 10 23:00:00 EST 2023 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.13995· OSTI ID:1908917

Wang, Zongkuan ^[1]; Yang, Bo ^[2]; Zheng, Wenyue ^[3]; Wang, Lei ^[3]; Cai, Xingxing ^[4]; Yang, Jie ^[5]; Song, Rongrong ^[4]; Yang, Sen ^[3]; Wang, Yuyin ^[3]; Xiao, Jin ^[4]; Liu, Huiquan ^[5]; ^[3]; ^[4]; ^[3]

College of Plant Protection Nanjing Agricultural University Nanjing Jiangsu China, State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics Institute, Nanjing Agricultural University/JCIC‐MCP Nanjing Jiangsu China
College of Plant Protection Nanjing Agricultural University Nanjing Jiangsu China, College of Grassland Science Nanjing Agricultural University Nanjing China, The Key Laboratory of Plant Immunity Nanjing Agricultural University Nanjing China
College of Plant Protection Nanjing Agricultural University Nanjing Jiangsu China, The Key Laboratory of Plant Immunity Nanjing Agricultural University Nanjing China
State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics Institute, Nanjing Agricultural University/JCIC‐MCP Nanjing Jiangsu China
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection Northwest A&,F University Yangling Shaanxi China

Summary

Fusarium head blight (FHB), caused by Fusarium graminearum , is a devastating disease in wheat ( Triticum aestivum ) that results in substantial yield losses and mycotoxin contamination. Reliable genetic resources for FHB resistance in wheat are lacking. In this study, we characterized glycoside hydrolase 12 (GH12) family proteins secreted by F. graminearum . We established that two GH12 proteins, Fg05851 and Fg11037, have functionally redundant roles in F. graminearum colonization of wheat. Furthermore, we determined that the GH12 proteins Fg05851 and Fg11037 are recognized by the leucine‐rich‐repeat receptor‐like protein RXEG1 in the dicot Nicotiana benthamiana . Heterologous expression of RXEG1 conferred wheat responsiveness to Fg05851 and Fg11037, enhanced wheat resistance to F. graminearum and reduced levels of the mycotoxin deoxynivalenol in wheat grains in an Fg05851/Fg11037‐dependent manner. In the RXEG1 transgenic lines, genes related to pattern‐triggered plant immunity, salicylic acid, jasmonic acid, and anti‐oxidative homeostasis signalling pathways were upregulated during F. graminearum infection. However, the expression of these genes was not significantly changed during infection by the deletion mutant ΔFg05851/Fg11037 , suggesting that the recognition of Fg05851/Fg11037 by RXEG1 triggered plant resistance against FHB. Moreover, introducing RXEG1 into three other different wheat cultivars via crossing also conferred resistance to F. graminearum . Expression of RXEG1 did not have obvious deleterious effects on plant growth and development in wheat. Our study reveals that N. benthamiana RXEG1 remains effective when transferred into wheat, a monocot, which in turn suggests that engineering wheat with interfamily plant immune receptor transgenes is a viable strategy for increasing resistance to FHB.

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Sponsoring Organization:: USDOE

OSTI ID:: 1908917

Alternate ID(s):: OSTI ID: 1963352

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 4 Vol. 21; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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