Planar 2D wireframe DNA origami

Wang, Xiao; Li, Shanshan; Jun, Hyungmin; John, Torsten; Zhang, Kaiming; Fowler, Hannah; Doye, Jonathan P.K.; Chiu, Wah; Bathe, Mark

doi:10.1126/sciadv.abn0039

Planar 2D wireframe DNA origami

Journal Article · Fri May 20 00:00:00 EDT 2022 · Science Advances

DOI:https://doi.org/10.1126/sciadv.abn0039· OSTI ID:1908739

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Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); SLAC
Stanford University, CA (United States); University of Science and Technology of China, Hefei (China)
Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); Jeonbuk National University, Jeonju (Korea, Republic of)
Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)
University of Oxford (United Kingdom)
Stanford University, CA (United States); SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Two-dimensional (2D) DNA origami is widely used for applications ranging from excitonics to single-molecule biophysics. Conventional, single-layer 2D DNA origami exhibits flexibility and curvature in solution; however, that may limit its suitability as a 2D structural template. In contrast, 2D wireframe DNA origami rendered with six-helix bundle edges offers local control over duplex orientations with enhanced in-plane rigidity. Here, we investigate the 3D structure of these assemblies using cryo–electron microscopy (cryo-EM). 3D reconstructions reveal a high degree of planarity and homogeneity in solution for polygonal objects with and without internal mesh, enabling 10-Å resolution for a triangle. Coarse-grained simulations were in agreement with cryo-EM data, offering molecular structural insight into this class of 2D DNA origami. Our results suggest that these assemblies may be valuable for 2D material applications and geometries that require high structural fidelity together with local control over duplex orientations, rather than parallel duplex assembly.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: Alexander von Humboldt Foundation; Army Research Office (ARO); Engineering and Physical Sciences Research Council (EPSRC); National Science Foundation (NSF); Office of Naval Research (ONR); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1908739

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 20 Vol. 8; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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