An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins

Rosi, Matthew; Russell, Brandon; Kristensen, Line G.; Farquhar, Erik R.; Jain, Rohit; Abel, Donald; Sullivan, Michael; Costello, Shawn M.; Dominguez-Martin, Maria Agustina; Chen, Yan; Marqusee, Susan; Petzold, Christopher J.; Kerfeld, Cheryl A.; DePonte, Daniel P.; Farahmand, Farid; Gupta, Sayan; Ralston, Corie Y.

doi:10.1038/s42003-022-03775-1

Title: An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins

Journal Article · Thu Aug 25 00:00:00 EDT 2022 · Communications Biology

DOI:https://doi.org/10.1038/s42003-022-03775-1· OSTI ID:1907590

Rosi, Matthew ^[1]; Russell, Brandon ^[1]; Kristensen, Line G. ^[2];

^[3];

^[3]; Abel, Donald ^[3]; Sullivan, Michael ^[3];

^[4]; Dominguez-Martin, Maria Agustina ^[5]; Chen, Yan ^[2];

^[4];

^[2];

^[5]; DePonte, Daniel P. ^[6]; Farahmand, Farid ^[1];

^[2];

^[2]

Sonoma State University, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Case Western Reserve University, Cleveland, OH (United States)
University of California, Berkeley, CA (United States)
Michigan State University, East Lansing, MI (United States)
SLAC National Accelerator Laboratory, Menlo Park, CA (United States)

X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density beams are essential to overcome radical scavengers. However, conventional sample delivery environments, such as capillary flow, limit the use of a fully unattenuated focused broadband beam. An alternative is to use a liquid jet, and we have previously demonstrated that use of this form of sample delivery can increase labeling by tenfold at an unfocused X-ray source. Here we report the first use of a liquid jet for automated inline quantitative fluorescence dosage characterization and sample exposure at a high flux density microfocused synchrotron beamline. Our approach enables exposure times in single-digit microseconds while retaining a high level of side-chain labeling. This development significantly boosts the method’s overall effectiveness and efficiency, generates high-quality data, and opens up the arena for high throughput and ultrafast time-resolved in situ hydroxyl radical labeling.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Case Western Reserve University; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231; SC0012704; DBI-1228549; P30-EB-009998; 1R01GM126218; 1R21AG072478; SC0020606; GM050945

OSTI ID:: 1907590

Journal Information:: Communications Biology, Vol. 5, Issue 1; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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