Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi

Richter, Felix; Bindschedler, Saskia; Calonne-Salmon, Maryline; Declerck, Stéphane; Junier, Pilar; Stanley, Claire E.

doi:10.1093/femsre/fuac039

Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi

Journal Article · Wed Aug 24 00:00:00 EDT 2022 · FEMS Microbiology Reviews

DOI:https://doi.org/10.1093/femsre/fuac039· OSTI ID:1906552

Richter, Felix; Bindschedler, Saskia; Calonne-Salmon, Maryline; Declerck, Stéphane; ;

Abstract

This review highlights new advances in the emerging field of ‘Fungi-on-a-Chip’ microfluidics for single-cell studies on fungi and discusses several future frontiers, where we envisage microfluidic technology development to be instrumental in aiding our understanding of fungal biology. Fungi, with their enormous diversity, bear essential roles both in nature and our everyday lives. They inhabit a range of ecosystems, such as soil, where they are involved in organic matter degradation and bioremediation processes. More recently, fungi have been recognized as key components of the microbiome in other eukaryotes, such as humans, where they play a fundamental role not only in human pathogenesis, but also likely as commensals. In the food sector, fungi are used either directly or as fermenting agents and are often key players in the biotechnological industry, where they are responsible for the production of both bulk chemicals and antibiotics. Although the macroscopic fruiting bodies are immediately recognizable by most observers, the structure, function, and interactions of fungi with other microbes at the microscopic scale still remain largely hidden. Herein, we shed light on new advances in the emerging field of Fungi-on-a-Chip microfluidic technologies for single-cell studies on fungi. We discuss the development and application of microfluidic tools in the fields of medicine and biotechnology, as well as in-depth biological studies having significance for ecology and general natural processes. Finally, a future perspective is provided, highlighting new frontiers in which microfluidic technology can benefit this field.

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Sponsoring Organization:: USDOE

OSTI ID:: 1906552

Alternate ID(s):: OSTI ID: 2422533
OSTI ID: 1891048

Journal Information:: FEMS Microbiology Reviews, Journal Name: FEMS Microbiology Reviews Journal Issue: 6 Vol. 46; ISSN 1574-6976

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (230)

PDMS microfluidics: A mini review Raj M., Kiran; Chakraborty, Suman Journal of Applied Polymer Science, Vol. 137, Issue 27 https://doi.org/10.1002/app.48958	journal	January 2020
A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments Luo, Chunxiong; Zhu, Xuejun; Yu, Tao Biotechnology and Bioengineering, Vol. 101, Issue 1 https://doi.org/10.1002/bit.21877	journal	September 2008
Evolutionary maintenance of genomic diversity within arbuscular mycorrhizal fungi Scott, Thomas W.; Kiers, E. Toby; Cooper, Guy A. Ecology and Evolution, Vol. 9, Issue 5 https://doi.org/10.1002/ece3.4834	journal	February 2019
Hybrid microfluidics combined with active and passive approaches for continuous cell separation: Microfluidics and Miniaturization Yan, Sheng; Zhang, Jun; Yuan, Dan ELECTROPHORESIS, Vol. 38, Issue 2 https://doi.org/10.1002/elps.201600386	journal	October 2016
Cultivation and quantitative single-cell analysis ofSaccharomyces cerevisiaeon a multifunctional microfluidic device Stratz, Simone; Verboket, Pascal Emilio; Hasler, Karina ELECTROPHORESIS, Vol. 39, Issue 3 https://doi.org/10.1002/elps.201700253	journal	October 2017
Recent advances in microfluidic cell sorting techniques based on both physical and biochemical principles Tang, Wenlai; Jiang, Di; Li, Zongan ELECTROPHORESIS, Vol. 40, Issue 6 https://doi.org/10.1002/elps.201800361	journal	October 2018
Fungi Use Efficient Algorithms for the Exploration of Microfluidic Networks Hanson, Kristi L.; Nicolau, Dan V.; Filipponi, Luisa Small, Vol. 2, Issue 10 https://doi.org/10.1002/smll.200600105	journal	August 2006
Microfluidic Platforms for Yeast-Based Aging Studies Jo, Myeong Chan; Qin, Lidong Small, Vol. 12, Issue 42 https://doi.org/10.1002/smll.201602006	journal	September 2016
Long-lived yeast as a model for ageing research Piper, Peter W. Yeast, Vol. 23, Issue 3 https://doi.org/10.1002/yea.1354	journal	January 2006
Microfluidics device for single cell gene expression analysis inSaccharomyces cerevisiae Ryley, James; Pereira-Smith, Olivia M. Yeast, Vol. 23, Issue 14-15 https://doi.org/10.1002/yea.1412	journal	January 2006
Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform Yu, Ruofan; Jo, Myeong Chan; Dang, Weiwei Aging https://doi.org/10.1007/978-1-0716-0592-9_1	book	January 2020
Droplet-Based Microfluidic High-Throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica Beneyton, Thomas; Rossignol, Tristan Methods in Molecular Biology https://doi.org/10.1007/978-1-0716-1414-3_14	book	January 2021
Preparation of Yeast Cells for Live-Cell Imaging and Indirect Immunofluorescence Pemberton, Lucy F. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-1363-3_6	book	January 2014
Organ on Chip Beißner, N.; Lorenz, T.; Reichl, S. Microsystems for Pharmatechnology https://doi.org/10.1007/978-3-319-26920-7_11	book	January 2016
Penicillin production by solid state fermentation Barrios-Gonz�lez, J.; Tomasini, A.; Viniegra-Gonz�lez, G. Biotechnology Letters, Vol. 10, Issue 11 https://doi.org/10.1007/BF01027575	journal	November 1988
Application of micro-segmented flow for two-dimensional characterization of the combinatorial effect of zinc and copper ions on metal-tolerant Streptomyces strains Cao, Jialan; Kürsten, Dana; Krause, Katrin Applied Microbiology and Biotechnology, Vol. 97, Issue 20 https://doi.org/10.1007/s00253-013-5147-8	journal	August 2013
New method for arbuscular mycorrhizal fungus spore separation using a microfluidic device based on manual temporary flow diversion Srisom, K.; Tittabutr, P.; Teaumroong, N. Mycorrhiza, Vol. 30, Issue 6 https://doi.org/10.1007/s00572-020-00986-4	journal	September 2020
The evolutionary phylogeny of the oomycete “fungi” Beakes, Gordon W.; Glockling, Sally L.; Sekimoto, Satoshi Protoplasma, Vol. 249, Issue 1 https://doi.org/10.1007/s00709-011-0269-2	journal	March 2011
Ultra-high-throughput picoliter-droplet microfluidics screening of the industrial cellulase-producing filamentous fungus Trichoderma reesei He, Ronglin; Ding, Ruihua; Heyman, John A. Journal of Industrial Microbiology and Biotechnology, Vol. 46, Issue 11 https://doi.org/10.1007/s10295-019-02221-2	journal	November 2019
Rapid prototyping of PMMA microfluidic chips utilizing a CO2 laser Hong, Ting-Fu; Ju, Wei-Jhong; Wu, Ming-Chang Microfluidics and Nanofluidics, Vol. 9, Issue 6 https://doi.org/10.1007/s10404-010-0633-0	journal	May 2010
Prospects of low temperature co-fired ceramic (LTCC) based microfluidic systems for point-of-care biosensing and environmental sensing Vasudev, Abhay; Kaushik, Ajeet; Jones, Kinzy Microfluidics and Nanofluidics, Vol. 14, Issue 3-4 https://doi.org/10.1007/s10404-012-1087-3	journal	November 2012
Numbering-up Y–Y microfluidic chips for higher-throughput solvent extraction of platinum(IV) chloride Kriel, Frederik H.; Woollam, Stephen; Gordon, Ross J. Microfluidics and Nanofluidics, Vol. 20, Issue 10 https://doi.org/10.1007/s10404-016-1802-6	journal	September 2016
Combined microfluidic-micromagnetic separation of living cells in continuous flow Xia, Nan; Hunt, Tom P.; Mayers, Brian T. Biomedical Microdevices, Vol. 8, Issue 4 https://doi.org/10.1007/s10544-006-0033-0	journal	September 2006
High-throughput microfluidic system for monitoring diffusion-based monolayer yeast cell culture over long time periods Luo, Chunxiong; Jiang, Lingli; Liang, Shibo Biomedical Microdevices, Vol. 11, Issue 5 https://doi.org/10.1007/s10544-009-9315-7	journal	April 2009
Improvement in cell capture throughput using parallel bioactivated microfluidic channels Javanmard, Mehdi; Babrzadeh, Farbod; Nyrén, Pål Biomedical Microdevices, Vol. 14, Issue 4 https://doi.org/10.1007/s10544-012-9643-x	journal	February 2012
Modelling Nutrient Uptake by Individual Hyphae of Arbuscular Mycorrhizal Fungi: Temporal and Spatial Scales for an Experimental Design Schnepf, Andrea; Jones, Davey; Roose, Tiina Bulletin of Mathematical Biology, Vol. 73, Issue 9 https://doi.org/10.1007/s11538-010-9617-1	journal	January 2011
Histochemical study of the insect cuticle infected by the fungus Entomophthora coronata Gabriel, Bernardo P. Journal of Invertebrate Pathology, Vol. 11, Issue 1 https://doi.org/10.1016/0022-2011(68)90056-6	journal	June 1968
Comparison of direct vs fumigation incubation microbial biomass estimates from ectomycorrhizal mat and non-mat soils Ingham, E. R.; Griffiths, R. P.; Cromack, K. Soil Biology and Biochemistry, Vol. 23, Issue 5 https://doi.org/10.1016/0038-0717(91)90011-8	journal	January 1991
Cyclosporin-A production by Tolypocladium inflatum using solid state fermentation Ramana Murthy, M. V.; Mohan, E. V. S.; Sadhukhan, A. K. Process Biochemistry, Vol. 34, Issue 3 https://doi.org/10.1016/S0032-9592(98)00095-8	journal	April 1999
Self/Nonself Recognition in Fungi: Old Mysteries and Simple Solutions Kahmann, Regine; Bölker, Michael Cell, Vol. 85, Issue 2 https://doi.org/10.1016/S0092-8674(00)81091-0	journal	April 1996
Luigi Galvani and animal electricity: two centuries after the foundation of electrophysiology Piccolino, M. Trends in Neurosciences, Vol. 20, Issue 10 https://doi.org/10.1016/S0166-2236(97)01101-6	journal	October 1997
Linking plants to rocks: ectomycorrhizal fungi mobilize nutrients from minerals Landeweert, Renske; Hoffland, Ellis; Finlay, Roger D. Trends in Ecology & Evolution, Vol. 16, Issue 5 https://doi.org/10.1016/S0169-5347(01)02122-X	journal	May 2001
Intracellular accommodation of microbes by plants: a common developmental program for symbiosis and disease? Parniske, Martin Current Opinion in Plant Biology, Vol. 3, Issue 4 https://doi.org/10.1016/S1369-5266(00)00088-1	journal	August 2000
Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation Bussamara, Roberta; Fuentefria, Alexandre Meneghello; Oliveira, Eder Silva de Bioresource Technology, Vol. 101, Issue 1 https://doi.org/10.1016/j.biortech.2008.10.063	journal	January 2010
Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes Hosokawa, Masahito; Hoshino, Yuri; Nishikawa, Yohei Biosensors and Bioelectronics, Vol. 67 https://doi.org/10.1016/j.bios.2014.08.059	journal	May 2015
Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential Fehrmann, Steffen; Paoletti, Camille; Goulev, Youlian Cell Reports, Vol. 5, Issue 6 https://doi.org/10.1016/j.celrep.2013.11.013	journal	December 2013
Yeast Replicator: A High-Throughput Multiplexed Microfluidics Platform for Automated Measurements of Single-Cell Aging Liu, Ping; Young, Thomas Z.; Acar, Murat Cell Reports, Vol. 13, Issue 3 https://doi.org/10.1016/j.celrep.2015.09.012	journal	October 2015
Oxalotrophic bacterial assemblages in the ectomycorrhizosphere of forest trees and their effects on oxalate degradation and carbon fixation potential Sun, Qibiao; Li, Jing; Finlay, Roger D. Chemical Geology, Vol. 514 https://doi.org/10.1016/j.chemgeo.2019.03.023	journal	June 2019
Mechanical Forces of Fission Yeast Growth Minc, Nicolas; Boudaoud, Arezki; Chang, Fred Current Biology, Vol. 19, Issue 13 https://doi.org/10.1016/j.cub.2009.05.031	journal	July 2009
Bidirectional Propagation of Signals and Nutrients in Fungal Networks via Specialized Hyphae Schmieder, Stefanie S.; Stanley, Claire E.; Rzepiela, Andrzej Current Biology, Vol. 29, Issue 2 https://doi.org/10.1016/j.cub.2018.11.058	journal	January 2019
Mycorrhizal networks: Mechanisms, ecology and modelling Simard, Suzanne W.; Beiler, Kevin J.; Bingham, Marcus A. Fungal Biology Reviews, Vol. 26, Issue 1 https://doi.org/10.1016/j.fbr.2012.01.001	journal	April 2012
Nuclear behavior in fungal hyphae Maheshwari, Ramesh FEMS Microbiology Letters, Vol. 249, Issue 1 https://doi.org/10.1016/j.femsle.2005.06.031	journal	August 2005
Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus resolution Lee, Kang Kug; Labiscsak, Laszlo; Ahn, Chong H. Fungal Genetics and Biology, Vol. 94 https://doi.org/10.1016/j.fgb.2016.06.004	journal	September 2016
Probing the growth dynamics of Neurospora crassa with microfluidic structures Held, Marie; Edwards, Clive; Nicolau, Dan V. Fungal Biology, Vol. 115, Issue 6 https://doi.org/10.1016/j.funbio.2011.02.003	journal	June 2011
Kidney-on-a-chip: untapped opportunities Ashammakhi, Nureddin; Wesseling-Perry, Katherine; Hasan, Anwarul Kidney International, Vol. 94, Issue 6 https://doi.org/10.1016/j.kint.2018.06.034	journal	December 2018
Microfluidic technologies for yeast replicative lifespan studies Chen, Kenneth L.; Crane, Matthew M.; Kaeberlein, Matt Mechanisms of Ageing and Development, Vol. 161 https://doi.org/10.1016/j.mad.2016.03.009	journal	January 2017
Molecular motors-based micro- and nano-biocomputation devices Nicolau, Dan V.; Nicolau, Dan V.; Solana, Gerardin Microelectronic Engineering, Vol. 83, Issue 4-9 https://doi.org/10.1016/j.mee.2006.01.198	journal	April 2006
Dynamic behaviour of microorganisms on microstructures Held, Marie; Komaromy, Andras; Fulga, Florin Microelectronic Engineering, Vol. 86, Issue 4-6 https://doi.org/10.1016/j.mee.2009.01.012	journal	April 2009
Microfluidics structures for probing the dynamic behaviour of filamentous fungi Held, Marie; Lee, Abraham P.; Edwards, Clive Microelectronic Engineering, Vol. 87, Issue 5-8 https://doi.org/10.1016/j.mee.2009.11.096	journal	May 2010
Mechanisms of hypha orientation of fungi Brand, Alexandra; Gow, Neil AR Current Opinion in Microbiology, Vol. 12, Issue 4 https://doi.org/10.1016/j.mib.2009.05.007	journal	August 2009
Self-signalling and self-fusion in filamentous fungi Read, Nick D.; Lichius, Alexander; Shoji, Jun-ya Current Opinion in Microbiology, Vol. 12, Issue 6 https://doi.org/10.1016/j.mib.2009.09.008	journal	December 2009
Regulatory mechanisms controlling morphology and pathogenesis in Candida albicans Kadosh, David Current Opinion in Microbiology, Vol. 52 https://doi.org/10.1016/j.mib.2019.04.005	journal	December 2019
A review on sustainable yeast biotechnological processes and applications Nandy, Subir Kumar; Srivastava, R. K. Microbiological Research, Vol. 207 https://doi.org/10.1016/j.micres.2017.11.013	journal	March 2018
Regulation of Cell Signaling Dynamics by the Protein Kinase-Scaffold Ste5 Hao, Nan; Nayak, Sujata; Behar, Marcelo Molecular Cell, Vol. 30, Issue 5 https://doi.org/10.1016/j.molcel.2008.04.016	journal	June 2008
Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli Lesage, Julie; Timoumi, Asma; Cenard, Stéphanie New Biotechnology, Vol. 64 https://doi.org/10.1016/j.nbt.2021.05.004	journal	September 2021
Cross-kingdom RNA trafficking and environmental RNAi for powerful innovative pre- and post-harvest plant protection Wang, Ming; Thomas, Nicholas; Jin, Hailing Current Opinion in Plant Biology, Vol. 38 https://doi.org/10.1016/j.pbi.2017.05.003	journal	August 2017
A compartmentalized microsystem helps understanding the uptake of benzo[a]pyrene by fungi during soil bioremediation processes Baranger, Claire; Pezron, Isabelle; Lins, Laurence Science of The Total Environment, Vol. 784 https://doi.org/10.1016/j.scitotenv.2021.147151	journal	August 2021
Hitchhikers on the fungal highway: The helper effect for bacterial migration via fungal hyphae Warmink, J. A.; Nazir, R.; Corten, B. Soil Biology and Biochemistry, Vol. 43, Issue 4 https://doi.org/10.1016/j.soilbio.2010.12.009	journal	April 2011
Spores-on-a-chip: new frontiers for spore research Bernier, Léa S.; Junier, Pilar; Stan, Guy-Bart Trends in Microbiology, Vol. 30, Issue 6 https://doi.org/10.1016/j.tim.2022.03.003	journal	June 2022
An inexpensive microscopy system for microfluidic studies in budding yeast Chen, Kenneth L.; Ven, Toby N.; Crane, Matthew M. Translational Medicine of Aging, Vol. 3 https://doi.org/10.1016/j.tma.2019.05.001	journal	January 2019
Plant neurobiology: an integrated view of plant signaling Brenner, Eric D.; Stahlberg, Rainer; Mancuso, Stefano Trends in Plant Science, Vol. 11, Issue 8 https://doi.org/10.1016/j.tplants.2006.06.009	journal	August 2006
Non-Mycorrhizal Plants: The Exceptions that Prove the Rule Cosme, Marco; Fernández, Ivan; Van der Heijden, Marcel G. A. Trends in Plant Science, Vol. 23, Issue 7 https://doi.org/10.1016/j.tplants.2018.04.004	journal	July 2018
Arbuscular mycorrhizal fungi conducting the hyphosphere bacterial orchestra Zhang, Lin; Zhou, Jiachao; George, Timothy S. Trends in Plant Science, Vol. 27, Issue 4 https://doi.org/10.1016/j.tplants.2021.10.008	journal	April 2022
Fungal behaviour: a new frontier in behavioural ecology Aleklett, Kristin; Boddy, Lynne Trends in Ecology & Evolution, Vol. 36, Issue 9 https://doi.org/10.1016/j.tree.2021.05.006	journal	September 2021
Introduction to Fungi Webster, John; Weber, Roland Cambridge University Press https://doi.org/10.1017/CBO9780511809026	book	January 2007
Microfluidic Platform for Liquid Chromatography−Tandem Mass Spectrometry Analyses of Complex Peptide Mixtures Xie, Jun; Miao, Yunan; Shih, Jason Analytical Chemistry, Vol. 77, Issue 21 https://doi.org/10.1021/ac0510888	journal	October 2005
Rapid Prototyping of Arrayed Microfluidic Systems in Polystyrene for Cell-Based Assays Young, Edmond W. K.; Berthier, Erwin; Guckenberger, David J. Analytical Chemistry, Vol. 83, Issue 4 https://doi.org/10.1021/ac102897h	journal	January 2011
Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress Lee, Myung Gwon; Shin, Joong Ho; Bae, Chae Yun Analytical Chemistry, Vol. 85, Issue 13 https://doi.org/10.1021/ac4006149	journal	June 2013
Versatile, Simple-to-Use Microfluidic Cell-Culturing Chip for Long-Term, High-Resolution, Time-Lapse Imaging Frey, Olivier; Rudolf, Fabian; Schmidt, Gregor W. Analytical Chemistry, Vol. 87, Issue 8 https://doi.org/10.1021/ac504611t	journal	April 2015
Synthesizing Living Tissues with Microfluidics Zheng, Wenfu; Jiang, Xingyu Accounts of Chemical Research, Vol. 51, Issue 12 https://doi.org/10.1021/acs.accounts.8b00417	journal	November 2018
Separation and Enrichment of Yeast Saccharomyces cerevisiae by Shape Using Viscoelastic Microfluidics Liu, Ping; Liu, Hangrui; Yuan, Dan Analytical Chemistry, Vol. 93, Issue 3 https://doi.org/10.1021/acs.analchem.0c03990	journal	December 2020
Precise Size-Based Cell Separation via the Coupling of Inertial Microfluidics and Deterministic Lateral Displacement Xiang, Nan; Wang, Jie; Li, Qiao Analytical Chemistry, Vol. 91, Issue 15 https://doi.org/10.1021/acs.analchem.9b02863	journal	July 2019
Microfluidics: Innovations in Materials and Their Fabrication and Functionalization Nielsen, Jacob B.; Hanson, Robert L.; Almughamsi, Haifa M. Analytical Chemistry, Vol. 92, Issue 1 https://doi.org/10.1021/acs.analchem.9b04986	journal	November 2019
Mycelial Effects on Phage Retention during Transport in a Microfluidic Platform Ghanem, Nawras; Stanley, Claire E.; Harms, Hauke Environmental Science & Technology, Vol. 53, Issue 20 https://doi.org/10.1021/acs.est.9b03502	journal	September 2019
Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array Duarte, Pedro A.; Menze, Lukas; Shoute, Lian ACS Omega, Vol. 7, Issue 1 https://doi.org/10.1021/acsomega.1c04878	journal	December 2021
Microfluidic Chip for Detection of Fungal Infections Asghar, Waseem; Sher, Mazhar; Khan, Nida S. ACS Omega, Vol. 4, Issue 4 https://doi.org/10.1021/acsomega.9b00499	journal	April 2019
Taking the Fungal Highway: Mobilization of Pollutant-Degrading Bacteria by Fungi Kohlmeier, Stefanie; Smits, Theo H. M.; Ford, Roseanne M. Environmental Science & Technology, Vol. 39, Issue 12 https://doi.org/10.1021/es047979z	journal	June 2005
Effect of Fungal Hyphae on the Access of Bacteria to Phenanthrene in Soil Wick, Lukas Y.; Remer, Rita; Würz, Birgit Environmental Science & Technology, Vol. 41, Issue 2 https://doi.org/10.1021/es061407s	journal	January 2007
Microfluidic Platform for Controlled Synthesis of Polymeric Nanoparticles Karnik, Rohit; Gu, Frank; Basto, Pamela Nano Letters, Vol. 8, Issue 9 https://doi.org/10.1021/nl801736q	journal	July 2008
Evolution of uni- and bifactorial sexual compatibility systems in fungi Nieuwenhuis, B. P. S.; Billiard, S.; Vuilleumier, S. Heredity, Vol. 111, Issue 6 https://doi.org/10.1038/hdy.2013.67	journal	July 2013
Promoter decoding of transcription factor dynamics involves a trade‐off between noise and control of gene expression Hansen, Anders S.; O'Shea, Erin K. Molecular Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1038/msb.2013.56	journal	January 2013
Monitoring dynamics of single‐cell gene expression over multiple cell cycles Cookson, Scott; Ostroff, Natalie; Pang, Wyming Lee Molecular Systems Biology, Vol. 1, Issue 1 https://doi.org/10.1038/msb4100032	journal	January 2005
Evolution of indirect reciprocity Nowak, Martin A.; Sigmund, Karl Nature, Vol. 437, Issue 7063 https://doi.org/10.1038/nature04131	journal	October 2005
MAPK-mediated bimodal gene expression and adaptive gradient sensing in yeast Paliwal, Saurabh; Iglesias, Pablo A.; Campbell, Kyle Nature, Vol. 446, Issue 7131 https://doi.org/10.1038/nature05561	journal	February 2007
Electrical signalling goes bacterial Beagle, Sarah D.; Lockless, Steve W. Nature, Vol. 527, Issue 7576 https://doi.org/10.1038/nature15641	journal	October 2015
Ion channels enable electrical communication in bacterial communities Prindle, Arthur; Liu, Jintao; Asally, Munehiro Nature, Vol. 527, Issue 7576 https://doi.org/10.1038/nature15709	journal	October 2015
Biopharmaceutical benchmarks 2014 Walsh, Gary Nature Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1038/nbt.3040	journal	October 2014
Man-made cell-like compartments for molecular evolution Tawfik, Dan S.; Griffiths, Andrew D. Nature Biotechnology, Vol. 16, Issue 7 https://doi.org/10.1038/nbt0798-652	journal	July 1998
Mycelium-mediated transfer of water and nutrients stimulates bacterial activity in dry and oligotrophic environments Worrich, Anja; Stryhanyuk, Hryhoriy; Musat, Niculina Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15472	journal	June 2017
An extracorporeal blood-cleansing device for sepsis therapy Kang, Joo H.; Super, Michael; Yung, Chong Wing Nature Medicine, Vol. 20, Issue 10 https://doi.org/10.1038/nm.3640	journal	September 2014
A microfluidic chemostat for experiments with bacterial and yeast cells Groisman, Alex; Lobo, Caroline; Cho, HoJung Nature Methods, Vol. 2, Issue 9 https://doi.org/10.1038/nmeth784	journal	August 2005
High-throughput microfluidics to control and measure signaling dynamics in single yeast cells Hansen, Anders S.; Hao, Nan; O'Shea, Erin K. Nature Protocols, Vol. 10, Issue 8 https://doi.org/10.1038/nprot.2015.079	journal	July 2015
Signal-dependent dynamics of transcription factor translocation controls gene expression Hao, Nan; O'Shea, Erin K. Nature Structural & Molecular Biology, Vol. 19, Issue 1 https://doi.org/10.1038/nsmb.2192	journal	December 2011
Signal beyond nutrient, fructose, exuded by an arbuscular mycorrhizal fungus triggers phytate mineralization by a phosphate solubilizing bacterium Zhang, Lin; Feng, Gu; Declerck, Stéphane The ISME Journal, Vol. 12, Issue 10 https://doi.org/10.1038/s41396-018-0171-4	journal	June 2018
Fungal foraging behaviour and hyphal space exploration in micro-structured Soil Chips Aleklett, Kristin; Ohlsson, Pelle; Bengtsson, Martin The ISME Journal, Vol. 15, Issue 6 https://doi.org/10.1038/s41396-020-00886-7	journal	January 2021
Phage co-transport with hyphal-riding bacteria fuels bacterial invasion in a water-unsaturated microbial model system You, Xin; Kallies, René; Kühn, Ingolf The ISME Journal, Vol. 16, Issue 5 https://doi.org/10.1038/s41396-021-01155-x	journal	December 2021
Microbial volatile communication in human organotypic lung models Barkal, Layla J.; Procknow, Clare L.; Álvarez-García, Yasmín R. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01985-4	journal	November 2017
Advances in organ-on-a-chip engineering Zhang, Boyang; Korolj, Anastasia; Lai, Benjamin Fook Lun Nature Reviews Materials, Vol. 3, Issue 8 https://doi.org/10.1038/s41578-018-0034-7	journal	August 2018
A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips Qiang, Le; Guo, Jing; Han, Yingkuan Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-44298-w	journal	May 2019
High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics Yaginuma, Kenshi; Aoki, Wataru; Miura, Natsuko Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-47388-x	journal	July 2019
High-Throughput Yeast Aging Analysis for Cryptococcus (HYAAC) microfluidic device streamlines aging studies in Cryptococcus neoformans Orner, Erika P.; Zhang, Pengchao; Jo, Myeong C. Communications Biology, Vol. 2, Issue 1 https://doi.org/10.1038/s42003-019-0504-5	journal	July 2019
A versatile microfluidic platform measures hyphal interactions between Fusarium graminearum and Clonostachys rosea in real-time Gimeno, Alejandro; Stanley, Claire E.; Ngamenie, Zacharie Communications Biology, Vol. 4, Issue 1 https://doi.org/10.1038/s42003-021-01767-1	journal	February 2021
Droplet-based microfluidic platform for high-throughput screening of Streptomyces Tu, Ran; Zhang, Yue; Hua, Erbing Communications Biology, Vol. 4, Issue 1 https://doi.org/10.1038/s42003-021-02186-y	journal	May 2021
Microfluidic chips provide visual access to in situ soil ecology Mafla-Endara, Paola Micaela; Arellano-Caicedo, Carlos; Aleklett, Kristin Communications Biology, Vol. 4, Issue 1 https://doi.org/10.1038/s42003-021-02379-5	journal	July 2021
High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics Beneyton, Thomas; Wijaya, I. Putu Mahendra; Postros, Prexilia Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep27223	journal	June 2016
Mycelia as a focal point for horizontal gene transfer among soil bacteria Berthold, Tom; Centler, Florian; Hübschmann, Thomas Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep36390	journal	November 2016
Rolled-up transparent microtubes as two-dimensionally confined culture scaffolds of individual yeast cells Huang, Gaoshan; Mei, Yongfeng; Thurmer, Dominic J. Lab Chip, Vol. 9, Issue 2 https://doi.org/10.1039/B810419K	journal	January 2009
High-throughput tracking of single yeast cells in a microfluidic imaging matrix Falconnet, D.; Niemistö, A.; Taylor, R. J. Lab Chip, Vol. 11, Issue 3 https://doi.org/10.1039/C0LC00228C	journal	January 2011
Surfactants in droplet-based microfluidics Baret, Jean-Christophe Lab Chip, Vol. 12, Issue 3 https://doi.org/10.1039/C1LC20582J	journal	January 2012
A microdevice for rapid optical detection of magnetically captured rare blood pathogens Cooper, Ryan M.; Leslie, Daniel C.; Domansky, Karel Lab Chip, Vol. 14, Issue 1 https://doi.org/10.1039/C3LC50935D	journal	January 2014
Oxygen levels in thermoplastic microfluidic devices during cell culture Ochs, Christopher J.; Kasuya, Junichi; Pavesi, Andrea Lab Chip, Vol. 14, Issue 3 https://doi.org/10.1039/C3LC51160J	journal	January 2014
Probing bacterial–fungal interactions at the single cell level Stanley, Claire E.; Stöckli, Martina; van Swaay, Dirk Integr. Biol., Vol. 6, Issue 10 https://doi.org/10.1039/C4IB00154K	journal	January 2014
Modulation of alpha-synuclein toxicity in yeast using a novel microfluidic-based gradient generator Fernandes, João Tiago S.; Tenreiro, Sandra; Gameiro, Andreia Lab Chip, Vol. 14, Issue 20 https://doi.org/10.1039/C4LC00756E	journal	January 2014
3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip Yoon No, Da; Lee, Kwang-Ho; Lee, Jaeseo Lab on a Chip, Vol. 15, Issue 19 https://doi.org/10.1039/C5LC00611B	journal	January 2015
Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants Wagner, Olaf; Thiele, Julian; Weinhart, Marie Lab on a Chip, Vol. 16, Issue 1 https://doi.org/10.1039/C5LC00823A	journal	January 2016
Soil-on-a-Chip: microfluidic platforms for environmental organismal studies Stanley, Claire E.; Grossmann, Guido; Casadevall i. Solvas, Xavier Lab on a Chip, Vol. 16, Issue 2 https://doi.org/10.1039/C5LC01285F	journal	January 2016
Leveraging a high resolution microfluidic assay reveals insights into pathogenic fungal spore germination Barkal, Layla J.; Walsh, Naomi M.; Botts, Michael R. Integrative Biology, Vol. 8, Issue 5 https://doi.org/10.1039/C6IB00012F	journal	January 2016
Microbial separation from a complex matrix by a hand-held microfluidic device Singh, Renu; Brockgreitens, John; Saiapina, Olga Chemical Communications, Vol. 53, Issue 78 https://doi.org/10.1039/C7CC06310E	journal	January 2017
Why microfluidics? Merits and trends in chemical synthesis Liu, Yong; Jiang, Xingyu Lab on a Chip, Vol. 17, Issue 23 https://doi.org/10.1039/C7LC00627F	journal	January 2017
Continuous separation of fungal spores in a microfluidic flow focusing device Park, Byeong Seon; Kye, Hyeon Gi; Kim, Tae Hyeon The Analyst, Vol. 144, Issue 16 https://doi.org/10.1039/C9AN00905A	journal	January 2019
Droplet incubation and splitting in open microfluidic channels Berry, Samuel B.; Lee, Jing J.; Berthier, Jean Analytical Methods, Vol. 11, Issue 35 https://doi.org/10.1039/C9AY00758J	journal	January 2019
High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq Liu, Leqian; Dalal, Chiraj K.; Heineike, Benjamin M. Lab on a Chip, Vol. 19, Issue 10 https://doi.org/10.1039/C9LC00084D	journal	January 2019
Microfluidic quantification and separation of yeast based on surface adhesion Reinmets, Kristina; Dehkharghani, Amin; Guasto, Jeffrey S. Lab on a Chip, Vol. 19, Issue 20 https://doi.org/10.1039/C9LC00275H	journal	January 2019
Quantitative analysis of yeast MAPK signaling networks and crosstalk using a microfluidic device Lee, Byungjin; Jeong, Seong-Geun; Jin, Si Hyung Lab on a Chip, Vol. 20, Issue 15 https://doi.org/10.1039/D0LC00203H	journal	January 2020
Microfluidic platform for integrated compartmentalization of single zoospores, germination and measurement of protrusive force generated by germ tubes Sun, Yiling; Tayagui, Ayelen; Garrill, Ashley Lab on a Chip, Vol. 20, Issue 22 https://doi.org/10.1039/D0LC00752H	journal	January 2020
Micro fluid segment technique for screening and development studies on Danio rerio embryos Funfak, Anette; Brösing, Andreas; Brand, Michael Lab on a Chip, Vol. 7, Issue 9 https://doi.org/10.1039/b701116d	journal	January 2007
Development of a microfluidic biochip for online monitoring of fungal biofilm dynamics Richter, Lukas; Stepper, Christoph; Mak, Andy Lab on a Chip, Vol. 7, Issue 12 https://doi.org/10.1039/b708236c	journal	January 2007
Micromagnetic–microfluidic blood cleansing device Yung, Chong Wing; Fiering, Jason; Mueller, Andrew J. Lab on a Chip, Vol. 9, Issue 9 https://doi.org/10.1039/b816986a	journal	January 2009
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity Baret, Jean-Christophe; Miller, Oliver J.; Taly, Valerie Lab on a Chip, Vol. 9, Issue 13, p. 1850-1858 https://doi.org/10.1039/b902504a	journal	January 2009
Bonding of microfluidic devices fabricated in polycarbonate Ogończyk, D.; Węgrzyn, J.; Jankowski, P. Lab on a Chip, Vol. 10, Issue 10 https://doi.org/10.1039/b924439e	journal	January 2010
Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions Bauer, Wolfgang-Andreas C.; Fischlechner, Martin; Abell, Chris Lab on a Chip, Vol. 10, Issue 14 https://doi.org/10.1039/c004046k	journal	January 2010
A modular approach for the generation, storage, mixing, and detection of droplet libraries for high throughput screening Trivedi, Varun; Doshi, Ankur; Kurup, G. K. Lab on a Chip, Vol. 10, Issue 18 https://doi.org/10.1039/c004768f	journal	January 2010
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics Pekin, Deniz; Skhiri, Yousr; Baret, Jean-Christophe Lab on a Chip, Vol. 11, Issue 13 https://doi.org/10.1039/c1lc20128j	journal	January 2011
Exploring emulsion science with microfluidics Bremond, Nicolas; Bibette, Jérôme Soft Matter, Vol. 8, Issue 41 https://doi.org/10.1039/c2sm25923k	journal	January 2012
Stimuli-responsive hydrogel patterns for smart microfluidics and microarrays Kang, Do Hyun; Kim, Sang Moon; Lee, Byungjun The Analyst, Vol. 138, Issue 21 https://doi.org/10.1039/c3an01119d	journal	January 2013
Microchemostat array with small-volume fraction replenishment for steady-state microbial culture Park, Jaewon; Wu, Jianzhang; Polymenis, Michael Lab on a Chip, Vol. 13, Issue 21 https://doi.org/10.1039/c3lc50665g	journal	January 2013
Branched absorbing structures (BAS): a feature of the extraradical mycelium of symbiotic arbuscular mycorrhizal fungi Bago, B.; Azcon-Aguilar, C.; Goulet, A. New Phytologist, Vol. 139, Issue 2 https://doi.org/10.1046/j.1469-8137.1998.00199.x	journal	June 1998
An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting Cheng, I-Fang; Chang, Hsien-Chang; Hou, Diana Biomicrofluidics, Vol. 1, Issue 2 https://doi.org/10.1063/1.2723669	journal	June 2007
High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment Rosa, Patrícia; Tenreiro, Sandra; Chu, Virginia Biomicrofluidics, Vol. 6, Issue 1 https://doi.org/10.1063/1.3683161	journal	March 2012
A microfluidics approach towards high-throughput pathogen removal from blood using margination Wei Hou, Han; Gan, Hiong Yap; Bhagat, Ali Asgar S. Biomicrofluidics, Vol. 6, Issue 2 https://doi.org/10.1063/1.4710992	journal	June 2012
Brain-on-a-chip: A history of development and future perspective Bang, Seokyoung; Jeong, Sohyeon; Choi, Nakwon Biomicrofluidics, Vol. 13, Issue 5 https://doi.org/10.1063/1.5120555	journal	September 2019
Chemotropism among populations of yeast cells with spatiotemporal resolution in a biofabricated microfluidic platform Vo, Thanh; Shah, Sameer B.; Choy, John S. Biomicrofluidics, Vol. 14, Issue 1 https://doi.org/10.1063/1.5128739	journal	January 2020
Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems Huh, D.; Fujioka, H.; Tung, Y. -C. Proceedings of the National Academy of Sciences, Vol. 104, Issue 48 https://doi.org/10.1073/pnas.0610868104	journal	November 2007
Signal processing by the HOG MAP kinase pathway Hersen, P.; McClean, M. N.; Mahadevan, L. Proceedings of the National Academy of Sciences, Vol. 105, Issue 20 https://doi.org/10.1073/pnas.0710770105	journal	May 2008
Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform Taylor, R. J.; Falconnet, D.; Niemistö, A. Proceedings of the National Academy of Sciences, Vol. 106, Issue 10 https://doi.org/10.1073/pnas.0813416106	journal	March 2009
Tracking lineages of single cells in lines using a microfluidic device Rowat, A. C.; Bird, J. C.; Agresti, J. J. Proceedings of the National Academy of Sciences, Vol. 106, Issue 43 https://doi.org/10.1073/pnas.0903163106	journal	October 2009
Droplet microfluidic technology for single-cell high-throughput screening Brouzes, E.; Medkova, M.; Savenelli, N. Proceedings of the National Academy of Sciences, Vol. 106, Issue 34 https://doi.org/10.1073/pnas.0903542106	journal	July 2009
High-resolution dose-response screening using droplet-based microfluidics Miller, O. J.; Harrak, A. E.; Mangeat, T. Proceedings of the National Academy of Sciences, Vol. 109, Issue 2 https://doi.org/10.1073/pnas.1113324109	journal	December 2011
Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform Lee, S. S.; Vizcarra, I. A.; Huberts, D. H. E. W. Proceedings of the National Academy of Sciences, Vol. 109, Issue 13 https://doi.org/10.1073/pnas.1113505109	journal	March 2012
Long-term model predictive control of gene expression at the population and single-cell levels Uhlendorf, J.; Miermont, A.; Delaveau, T. Proceedings of the National Academy of Sciences, Vol. 109, Issue 35 https://doi.org/10.1073/pnas.1206810109	journal	August 2012
Dissecting genealogy and cell cycle as sources of cell-to-cell variability in MAPK signaling using high-throughput lineage tracking Ricicova, Marketa; Hamidi, Mani; Quiring, Adam Proceedings of the National Academy of Sciences, Vol. 110, Issue 28 https://doi.org/10.1073/pnas.1215850110	journal	June 2013
A chemostat array enables the spatio-temporal analysis of the yeast proteome Denervaud, N.; Becker, J.; Delgado-Gonzalo, R. Proceedings of the National Academy of Sciences, Vol. 110, Issue 39 https://doi.org/10.1073/pnas.1308265110	journal	September 2013
Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae Huberts, Daphne H. E. W.; González, Javier; Lee, Sung Sik Proceedings of the National Academy of Sciences, Vol. 111, Issue 32 https://doi.org/10.1073/pnas.1410024111	journal	July 2014
High-throughput analysis of yeast replicative aging using a microfluidic system Jo, Myeong Chan; Liu, Wei; Gu, Liang Proceedings of the National Academy of Sciences, Vol. 112, Issue 30 https://doi.org/10.1073/pnas.1510328112	journal	July 2015
Intracellular mechanisms of fungal space searching in microenvironments Held, Marie; Kašpar, Ondřej; Edwards, Clive Proceedings of the National Academy of Sciences, Vol. 116, Issue 27 https://doi.org/10.1073/pnas.1816423116	journal	June 2019
RNAi expression tuning, microfluidic screening, and genome recombineering for improved protein production in Saccharomyces cerevisiae Wang, Guokun; Björk, Sara M.; Huang, Mingtao Proceedings of the National Academy of Sciences, Vol. 116, Issue 19 https://doi.org/10.1073/pnas.1820561116	journal	April 2019
Lung-on-a-chip: the future of respiratory disease models and pharmacological studies Shrestha, Jesus; Razavi Bazaz, Sajad; Aboulkheyr Es, Hamidreza Critical Reviews in Biotechnology, Vol. 40, Issue 2 https://doi.org/10.1080/07388551.2019.1710458	journal	January 2020
Overview of citric acid production from Aspergillus niger Show, Pau Loke; Oladele, Kehinde Opeyemi; Siew, Qi Yan Frontiers in Life Science, Vol. 8, Issue 3 https://doi.org/10.1080/21553769.2015.1033653	journal	April 2015
A micropillar-based on-chip system for continuous force measurement ofC. elegans Ghanbari, Ali; Nock, Volker; Johari, Shazlina Journal of Micromechanics and Microengineering, Vol. 22, Issue 9 https://doi.org/10.1088/0960-1317/22/9/095009	journal	July 2012
Fungal intelligence; Or on the behaviour of microorganisms in confined micro-environments Held, M.; Edwards, C.; Nicolau, D. V. Journal of Physics: Conference Series, Vol. 178 https://doi.org/10.1088/1742-6596/178/1/012005	journal	July 2009
Affinity Chromatography: A Review of Clinical Applications Hage, David S. Clinical Chemistry, Vol. 45, Issue 5 https://doi.org/10.1093/clinchem/45.5.593	journal	May 1999
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization Griffiths, A. D. The EMBO Journal, Vol. 22, Issue 1 https://doi.org/10.1093/emboj/cdg014	journal	January 2003
Yarrowia lipolytica: a model yeast for citric acid production Cavallo, Ema; Charreau, Hernán; Cerrutti, Patricia FEMS Yeast Research, Vol. 17, Issue 8 https://doi.org/10.1093/femsyr/fox084	journal	November 2017
Penetration of the Human Pulmonary Epithelium by Aspergillus fumigatus Hyphae Fernandes, Julien; Hamidi, Fatima; Leborgne, Remi The Journal of Infectious Diseases, Vol. 218, Issue 8 https://doi.org/10.1093/infdis/jiy298	journal	May 2018
Stable propagation of cosmid sized human DNA inserts in an F factor based vector Kim, Ung-Jin; Shizuya, Hiroaki; de Jong, Pieter J. Nucleic Acids Research, Vol. 20, Issue 5 https://doi.org/10.1093/nar/20.5.1083	journal	January 1992
A Unified Effort to Fight an Enemy of Wheat and Barley: Fusarium Head Blight McMullen, Marcia; Bergstrom, Gary; De Wolf, Erick Plant Disease, Vol. 96, Issue 12 https://doi.org/10.1094/PDIS-03-12-0291-FE	journal	December 2012
Microfluidic monitoring of the growth of individual hyphae in confined environments Baranger, Claire; Fayeulle, Antoine; Le Goff, Anne Royal Society Open Science, Vol. 7, Issue 8 https://doi.org/10.1098/rsos.191535	journal	August 2020
Language of fungi derived from their electrical spiking activity Adamatzky, Andrew Royal Society Open Science, Vol. 9, Issue 4 https://doi.org/10.1098/rsos.211926	journal	April 2022
Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets Bringer, Michelle R.; Gerdts, Cory J.; Song, Helen Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences, Vol. 362, Issue 1818 https://doi.org/10.1098/rsta.2003.1364	journal	May 2004
Changing perspectives in yeast research nearly a decade after the genome sequence Dolinski, Kara; Botstein, David Genome Research, Vol. 15, Issue 12 https://doi.org/10.1101/gr.3727505	journal	December 2005
The RootChip: An Integrated Microfluidic Chip for Plant Science Grossmann, Guido; Guo, Woei-Jiun; Ehrhardt, David W. The Plant Cell, Vol. 23, Issue 12 https://doi.org/10.1105/tpc.111.092577	journal	December 2011
Fabrication of In-Channel High-Aspect Ratio Sensing Pillars for Protrusive Force Measurements on Fungi and Oomycetes Sun, Yiling; Tayagui, Ayelen; Garrill, Ashley Journal of Microelectromechanical Systems, Vol. 27, Issue 5 https://doi.org/10.1109/JMEMS.2018.2862863	journal	October 2018
Marine yeast isolation and industrial application Zaky, Abdelrahman Saleh; Tucker, Gregory A.; Daw, Zakaria Yehia FEMS Yeast Research, Vol. 14, Issue 6 https://doi.org/10.1111/1567-1364.12158	journal	May 2014
Ecological and evolutionary implications of hyphal anastomosis in arbuscular mycorrhizal fungi Chagnon, Pierre-Luc FEMS Microbiology Ecology, Vol. 88, Issue 3 https://doi.org/10.1111/1574-6941.12321	journal	April 2014
Two‐component system in Rahnella aquatilis is impacted by the hyphosphere of the arbuscular mycorrhizal fungus Rhizophagus irregularis Duan, Shilong; Declerck, Stéphane; Zhang, Lin Environmental Microbiology Reports, Vol. 14, Issue 1 https://doi.org/10.1111/1758-2229.13039	journal	December 2021
Contact-induced apical asymmetry drives the thigmotropic responses ofCandida albicanshyphae Thomson, Darren D.; Wehmeier, Silvia; Byfield, FitzRoy J. Cellular Microbiology, Vol. 17, Issue 3 https://doi.org/10.1111/cmi.12369	journal	November 2014
Underground signals carried through common mycelial networks warn neighbouring plants of aphid attack Babikova, Zdenka; Gilbert, Lucy; Bruce, Toby J. A. Ecology Letters, Vol. 16, Issue 7 https://doi.org/10.1111/ele.12115	journal	May 2013
One fungus, one name promotes progressive plant pathology: One fungus, one name Wingfield, Michael J.; De Beer, Z. Wilhelm; Slippers, Bernard Molecular Plant Pathology, Vol. 13, Issue 6 https://doi.org/10.1111/j.1364-3703.2011.00768.x	journal	December 2011
Arbuscular mycorrhizal fungi as support systems for seedling establishment in grassland Van Der Heijden, Marcel G. A. Ecology Letters, Vol. 7, Issue 4 https://doi.org/10.1111/j.1461-0248.2004.00577.x	journal	March 2004
Fungi, bacteria and soil pH: the oxalate-carbonate pathway as a model for metabolic interaction: Bacteria/fungi interactions and soil pH Martin, Gaëtan; Guggiari, Matteo; Bravo, Daniel Environmental Microbiology, Vol. 14, Issue 11 https://doi.org/10.1111/j.1462-2920.2012.02862.x	journal	August 2012
Arbuscular mycorrhizal fungi reveal distinct patterns of anastomosis formation and hyphal healing mechanisms between different phylogenic groups De La Providencia, Ivan Enrique; De Souza, Francisco Adriano; Fernández, Félix New Phytologist, Vol. 165, Issue 1 https://doi.org/10.1111/j.1469-8137.2004.01236.x	journal	November 2004
Glomeraceae and Gigasporaceae differ in their ability to form hyphal networks Voets, Liesbeth; de la Providencia, Ivan Enrique; Declerck, Stéphane New Phytologist, Vol. 172, Issue 2 https://doi.org/10.1111/j.1469-8137.2006.01873.x	journal	October 2006
Nonself vegetative fusion and genetic exchange in the arbuscular mycorrhizal fungus Glomus intraradices Croll, Daniel; Giovannetti, Manuela; Koch, Alexander M. New Phytologist, Vol. 181, Issue 4 https://doi.org/10.1111/j.1469-8137.2008.02726.x	journal	December 2008
Activity of mannose-binding lectin in centenarians Tomaiuolo, Rossella; Ruocco, Anna; Salapete, Chiara Aging Cell, Vol. 11, Issue 3 https://doi.org/10.1111/j.1474-9726.2012.00793.x	journal	February 2012
Molecular phenotyping of aging in single yeast cells using a novel microfluidic device Xie, Zhengwei; Zhang, Yi; Zou, Ke Aging Cell, Vol. 11, Issue 4 https://doi.org/10.1111/j.1474-9726.2012.00821.x	journal	May 2012
The fungal α‐aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate Fazius, Felicitas; Shelest, Ekaterina; Gebhardt, Peter Molecular Microbiology, Vol. 86, Issue 6 https://doi.org/10.1111/mmi.12076	journal	November 2012
Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea Stöckli, Martina; Morinaka, Brandon I.; Lackner, Gerald Molecular Microbiology, Vol. 112, Issue 2 https://doi.org/10.1111/mmi.14277	journal	May 2019
Microfabrication and its use in investigating fungal biology Bedekovic, Tina; Brand, Alexandra C. Molecular Microbiology, Vol. 117, Issue 3 https://doi.org/10.1111/mmi.14816	journal	October 2021
Dual-flow-RootChip reveals local adaptations of roots towards environmental asymmetry at the physiological and genetic levels Stanley, Claire E.; Shrivastava, Jagriti; Brugman, Rik New Phytologist, Vol. 217, Issue 3 https://doi.org/10.1111/nph.14887	journal	November 2017
Arbuscular mycorrhizal fungi enhance mineralisation of organic phosphorus by carrying bacteria along their extraradical hyphae Jiang, Feiyan; Zhang, Lin; Zhou, Jiachao New Phytologist, Vol. 230, Issue 1 https://doi.org/10.1111/nph.17081	journal	January 2021
Dynamic behaviour of fungi in microfluidics: a comparative study Held, Marie; Binz, Marie; Edwards, Clive SPIE Proceedings https://doi.org/10.1117/12.822464	conference	February 2009
Reconstituting Organ-Level Lung Functions on a Chip Huh, D.; Matthews, B. D.; Mammoto, A. Science, Vol. 328, Issue 5986 https://doi.org/10.1126/science.1188302	journal	June 2010
Tunable Signal Processing Through Modular Control of Transcription Factor Translocation Hao, N.; Budnik, B. A.; Gunawardena, J. Science, Vol. 339, Issue 6118 https://doi.org/10.1126/science.1227299	journal	January 2013
Detection and Learning of Floral Electric Fields by Bumblebees Clarke, Dominic; Whitney, Heather; Sutton, Gregory Science, Vol. 340, Issue 6128 https://doi.org/10.1126/science.1230883	journal	February 2013
New Concepts of Kingdoms of Organisms Whittaker, R. H. Science, Vol. 163, Issue 3863 https://doi.org/10.1126/science.163.3863.150	journal	January 1969
Delaying the Empiric Treatment of Candida Bloodstream Infection until Positive Blood Culture Results Are Obtained: a Potential Risk Factor for Hospital Mortality Morrell, M.; Fraser, V. J.; Kollef, M. H. Antimicrobial Agents and Chemotherapy, Vol. 49, Issue 9 https://doi.org/10.1128/AAC.49.9.3640-3645.2005	journal	August 2005
New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria Andersen, Jens Bo; Sternberg, Claus; Poulsen, Lars Kongsbak Applied and Environmental Microbiology, Vol. 64, Issue 6 https://doi.org/10.1128/AEM.64.6.2240-2246.1998	journal	June 1998
Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures Zhang, Sean X.; Carroll, Karen C.; Lewis, Shawna Journal of Clinical Microbiology, Vol. 58, Issue 5 https://doi.org/10.1128/JCM.02096-19	journal	April 2020
Trade-off between Plasticity and Velocity in Mycelial Growth Fukuda, Sayumi; Yamamoto, Riho; Yanagisawa, Naoki mBio, Vol. 12, Issue 2 https://doi.org/10.1128/mBio.03196-20	journal	April 2021
Fungal Diversity Revisited: 2.2 to 3.8 Million Species Hawksworth, David L.; Lücking, Robert Microbiology Spectrum, Vol. 5, Issue 4 https://doi.org/10.1128/microbiolspec.FUNK-0052-2016	journal	August 2017
Fungi and the evolution of growth form Andrews, John H. Canadian Journal of Botany, Vol. 73, Issue S1 https://doi.org/10.1139/b95-380	journal	December 1995
Development of Image Processing Program for Yeast cell Morphology Ohtani, Miwaka; Saka, Ayaka; Sano, Fumi Journal of Bioinformatics and Computational Biology, Vol. 01, Issue 04 https://doi.org/10.1142/S0219720004000363	journal	January 2004
Digital Microfluidics Choi, Kihwan; Ng, Alphonsus H. C.; Fobel, Ryan Annual Review of Analytical Chemistry, Vol. 5, Issue 1 https://doi.org/10.1146/annurev-anchem-062011-143028	journal	July 2012
The History of Poiseuille's Law Sutera, S. P.; Skalak, R. Annual Review of Fluid Mechanics, Vol. 25, Issue 1 https://doi.org/10.1146/annurev.fl.25.010193.000245	journal	January 1993
Artificial Dirt: Microfluidic Substrates for Nematode Neurobiology and Behavior Lockery, S. R.; Lawton, K. J.; Doll, J. C. Journal of Neurophysiology, Vol. 99, Issue 6 https://doi.org/10.1152/jn.91327.2007	journal	June 2008
A microfluidic device to acquire high-magnification microphotographs of yeast cells Ohnuki, Shinsuke; Nogami, Satoru; Ohya, Yoshikazu Cell Division, Vol. 4, Issue 1 https://doi.org/10.1186/1747-1028-4-5	journal	March 2009
Combining microfluidics and RNA-sequencing to assess the inducible defensome of a mushroom against nematodes Tayyrov, Annageldi; Stanley, Claire E.; Azevedo, Sophie BMC Genomics, Vol. 20, Issue 1 https://doi.org/10.1186/s12864-019-5607-3	journal	March 2019
Mechanical force-induced morphology changes in a human fungal pathogen Puerner, Charles; Kukhaleishvili, Nino; Thomson, Darren BMC Biology, Vol. 18, Issue 1 https://doi.org/10.1186/s12915-020-00833-0	journal	September 2020
Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons Strom, Noah B.; Bushley, Kathryn E. Fungal Biology and Biotechnology, Vol. 3, Issue 1 https://doi.org/10.1186/s40694-016-0022-x	journal	May 2016
A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells Charvin, Gilles; Cross, Frederick R.; Siggia, Eric D. PLoS ONE, Vol. 3, Issue 1 https://doi.org/10.1371/journal.pone.0001468	journal	January 2008
Robust Spatial Sensing of Mating Pheromone Gradients by Yeast Cells Moore, Travis I.; Chou, Ching-Shan; Nie, Qing PLoS ONE, Vol. 3, Issue 12 https://doi.org/10.1371/journal.pone.0003865	journal	December 2008
Single Cell Analysis of Yeast Replicative Aging Using a New Generation of Microfluidic Device Zhang, Yi; Luo, Chunxiong; Zou, Ke PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0048275	journal	November 2012
Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array Nobs, Jean-Bernard; Maerkl, Sebastian J. PLoS ONE, Vol. 9, Issue 4 https://doi.org/10.1371/journal.pone.0093466	journal	April 2014
A Microfluidic System for Studying Ageing and Dynamic Single-Cell Responses in Budding Yeast Crane, Matthew M.; Clark, Ivan B. N.; Bakker, Elco PLoS ONE, Vol. 9, Issue 6 https://doi.org/10.1371/journal.pone.0100042	journal	June 2014
Crowdsourced analysis of fungal growth and branching on microfluidic platforms Hopke, Alex; Mela, Alex; Ellett, Felix PLOS ONE, Vol. 16, Issue 9 https://doi.org/10.1371/journal.pone.0257823	journal	September 2021
A Human Breathing Lung-on-a-Chip Huh, Dongeun (Dan) Annals of the American Thoracic Society, Vol. 12, Issue Supplement 1 https://doi.org/10.1513/AnnalsATS.201410-442MG	journal	March 2015
A simple microfluidic platform to study age-dependent protein abundance and localization changes in Saccharomyces cerevisiae Cabrera, Margarita; Novarina, Daniele; Rempel, Irina L. Microbial Cell, Vol. 4, Issue 5 https://doi.org/10.15698/mic2017.05.573	journal	May 2017
A microfluidic system for dynamic yeast cell imaging Lee, Philip J.; Helman, Noah C.; Lim, Wendell A. BioTechniques, Vol. 44, Issue 1 https://doi.org/10.2144/000112673	journal	January 2008
The Whispering World of Plants: ‘The Wood Wide Web’ Rhodes, Christopher J. Science Progress, Vol. 100, Issue 3 https://doi.org/10.3184/003685017X14968299580423	journal	September 2017
Droplet-Based Microfluidic High Throughput Screening of Corynebacterium glutamicum for Efficient Heterologous Protein Production and Secretion Balasubramanian, Suvasini; Chen, Jun; Wigneswaran, Vinoth Frontiers in Bioengineering and Biotechnology, Vol. 9 https://doi.org/10.3389/fbioe.2021.668513	journal	May 2021
Rapid Isolation and Concentration of Pathogenic Fungi Using Inertial Focusing on a Chip-Based Platform Fuchs, Beth Burgwyn; Eatemadpour, Soraya; Martel-Foley, Joseph M. Frontiers in Cellular and Infection Microbiology, Vol. 9 https://doi.org/10.3389/fcimb.2019.00027	journal	February 2019
Using Lung Organoids to Investigate Epithelial Barrier Complexity and IL-17 Signaling During Respiratory Infection Paolicelli, Giuseppe; Luca, Antonella De; Jose, Shyam S. Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.00323	journal	February 2019
The Still Underestimated Problem of Fungal Diseases Worldwide Almeida, Fausto; Rodrigues, Marcio L.; Coelho, Carolina Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00214	journal	February 2019
Microfluidics and Metabolomics Reveal Symbiotic Bacterial–Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange Uehling, Jessie K.; Entler, Matthew R.; Meredith, Hannah R. Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02163	journal	October 2019
The Lichens’ Microbiota, Still a Mystery? Grimm, Maria; Grube, Martin; Schiefelbein, Ulf Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.623839	journal	March 2021
Pseudomonas Strains Induce Transcriptional and Morphological Changes and Reduce Root Colonization of Verticillium spp. Harting, Rebekka; Nagel, Alexandra; Nesemann, Kai Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.652468	journal	May 2021
The Integration of Electrical Signals Originating in the Root of Vascular Plants Canales, Javier; Henriquez-Valencia, Carlos; Brauchi, Sebastian Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.02173	journal	January 2018
Fungicides With Contrasting Mode of Action Differentially Affect Hyphal Healing Mechanism in Gigaspora sp. and Rhizophagus irregularis Rodriguez-Morelos, Victor Hugo; Calonne-Salmon, Maryline; Bremhorst, Vincent Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.642094	journal	March 2021
Airway-On-A-Chip: Designs and Applications for Lung Repair and Disease Bennet, Tanya J.; Randhawa, Avineet; Hua, Jessica Cells, Vol. 10, Issue 7 https://doi.org/10.3390/cells10071602	journal	June 2021
The Collection Method for Crop Fungal Spores Based on an Efficient Microfluidic Device Wang, Pan; Yuan, Shouqi; Yang, Ning Aerosol and Air Quality Research, Vol. 20, Issue 1 https://doi.org/10.4209/aaqr.2019.08.0424	journal	January 2020

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