Behavioral and neuroinflammatory changes caused by glyphosate: Base herbicide in mice offspring

de Castro Vieira Carneiro, Carla Larissa; Chaves, Edna Maria Camelo; Neves, Kelly Rose Tavares; Braga, Marcus Davis Machado; Assreuy, Ana Maria Sampaio; de Moraes, Maria Elisabete Amaral; Aragão, Gislei Frota

doi:10.1002/bdr2.2138

Behavioral and neuroinflammatory changes caused by glyphosate: Base herbicide in mice offspring

Journal Article · Sun Dec 18 00:00:00 EST 2022 · Birth Defects Research

DOI:https://doi.org/10.1002/bdr2.2138· OSTI ID:1905670

de Castro Vieira Carneiro, Carla Larissa ^[1]; Chaves, Edna Maria Camelo ^[1]; Neves, Kelly Rose Tavares ^[2]; Braga, Marcus Davis Machado ^[2]; Assreuy, Ana Maria Sampaio ^[1]; de Moraes, Maria Elisabete Amaral ^[2]; ^[3]

Instituto Superior de Ciências Biomédicas (ISCB), State University of Ceará Ceará Brazil
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM) Federal University of Ceará Ceará Brazil
Instituto Superior de Ciências Biomédicas (ISCB), State University of Ceará Ceará Brazil, Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM) Federal University of Ceará Ceará Brazil

Abstract Background

Glyphosate is a pesticide considered of low toxicity, but scientific evidences show it can be harmful to health. This study aimed to evaluate the toxicity in mice offspring exposed to glyphosate‐based herbicide (GBH) during the intrauterine period.

Methods

Female matrices received glyphosate 0.3 mg/kg daily per oral throughout the gestational period, which was variable between 18 and 22 days. From the 25th until the 28th days post‐birth, mice offspring were subjected to behavioral tests, and the prefrontal cortex was processed for immunohistochemical analysis.

Results

Two significant behavioral changes were observed: anxiety in the GLIF0.3 group, increase in the behavior burying marbles in the marble‐burying test and hyperactivity, expressed by the significant increase of the crossing number in the open field test. The increased microglia, TNF‐alpha, and astrocyte expression were also observed in the prefrontal cortex of offspring treated with GLIF0.3.

Conclusion

Exposure to GBH during mice intrauterine development induces hyperactive and anxious behavior, evidencing neuroinflammation.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

OSTI ID:: 1905670

Journal Information:: Birth Defects Research, Journal Name: Birth Defects Research Journal Issue: 4 Vol. 115; ISSN 2472-1727

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Country unknown/Code not available

Language:: English

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