Crystal structure of a far-red–sensing cyanobacteriochrome reveals an atypical bilin conformation and spectral tuning mechanism

Bandara, Sepalika; Rockwell, Nathan C.; Zeng, Xiaoli; Ren, Zhong; Wang, Cong; Shin, Heewhan; Martin, Shelley S.; Moreno, Marcus V.; Lagarias, J. Clark; Yang, Xiaojing

doi:10.1073/pnas.2025094118

Crystal structure of a far-red–sensing cyanobacteriochrome reveals an atypical bilin conformation and spectral tuning mechanism

Journal Article · Tue Mar 16 00:00:00 UTC 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.2025094118· OSTI ID:1771232

Bandara, Sepalika ^[1]; ^[2]; Zeng, Xiaoli ^[1]; ^[1]; Wang, Cong ^[1]; Shin, Heewhan ^[1]; Martin, Shelley S. ^[2]; ^[2]; ^[2]; ^[3]

Department of Chemistry, University of Illinois, Chicago, IL 60607,
Department of Molecular and Cellular Biology, University of California, Davis, CA 95616,
Department of Chemistry, University of Illinois, Chicago, IL 60607,, Department of Ophthalmology and Vision Sciences, University of Illinois, Chicago, IL 60607

Significance

Phytochromes are well-known far-red-light sensors found in plants that trigger adaptive responses to facilitate competition for light capture with neighboring plants. Red- and far-red sensing are also critical for cyanobacteria living in the far-red–enriched shade of plants. This work reports the crystal structure of a far-red–sensing cyanobacteriochrome, a distant cyanobacterial relative of phytochrome. These studies shed insights into the molecular basis of far-red-sensing by phycobilin-based photoreceptors. Owing to the deep tissue penetration of far-red light, far-red–sensing cyanobacteriochromes are promising protein scaffolds for developing genetically encoded photoswitches, optoacoustic contrast agents, and fluorescent probes for in situ imaging and optogenetic applications.

View Journal Article

Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0002395

OSTI ID:: 1771232

Alternate ID(s):: OSTI ID: 1772767
OSTI ID: 1773625
OSTI ID: 1905445

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 12 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Atomic-resolution structure of the phycocyanobilin:ferredoxin oxidoreductase I86D mutant in complex with fully protonated biliverdin Hagiwara, Yoshinori; Wada, Kei; Irikawa, Teppei FEBS Letters, Vol. 590, Issue 19 https://doi.org/10.1002/1873-3468.12387	journal	September 2016
Fused-Gene Approach to Photoswitchable and Fluorescent Biliproteins Zhang, Juan; Wu, Xian-Jun; Wang, Zhi-Bin Angewandte Chemie International Edition, Vol. 49, Issue 32 https://doi.org/10.1002/anie.201001094	journal	June 2010
Femtosecond Dynamics in the Lactim Tautomer of Phycocyanobilin: A Long-Wavelength Absorbing Model Compound for the Phytochrome Chromophore Singer, Patrick; Fey, Sonja; Göller, Andreas H. ChemPhysChem, Vol. 15, Issue 17 https://doi.org/10.1002/cphc.201402383	journal	September 2014
Geometry versus basicity of bilatrienes: Stretched and helical protonated biliverdins Krois, Daniel Monatshefte f�r Chemie Chemical Monthly, Vol. 122, Issue 6-7 https://doi.org/10.1007/BF00809802	journal	January 1991
Allophycocyanin and phycocyanin crystal structures reveal facets of phycobilisome assembly Marx, Ailie; Adir, Noam Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 3 https://doi.org/10.1016/j.bbabio.2012.11.006	journal	March 2013
Multichromatic Control of Gene Expression in Escherichia coli Tabor, Jeffrey J.; Levskaya, Anselm; Voigt, Christopher A. Journal of Molecular Biology, Vol. 405, Issue 2 https://doi.org/10.1016/j.jmb.2010.10.038	journal	January 2011
Diverse Chromatic Acclimation Processes Regulating Phycoerythrocyanin and Rod-Shaped Phycobilisome in Cyanobacteria Hirose, Yuu; Chihong, Song; Watanabe, Mai Molecular Plant, Vol. 12, Issue 5 https://doi.org/10.1016/j.molp.2019.02.010	journal	May 2019
Biliproteins and their Applications in Bioimaging Scheer, Hugo; Yang, Xiaojing; Zhao, Kai-Hong Procedia Chemistry, Vol. 14 https://doi.org/10.1016/j.proche.2015.03.026	journal	January 2015
Cyanobacteriochromes: photoreceptors covering the entire UV-to-visible spectrum Fushimi, Keiji; Narikawa, Rei Current Opinion in Structural Biology, Vol. 57 https://doi.org/10.1016/j.sbi.2019.01.018	journal	August 2019
A Photo-Labile Thioether Linkage to Phycoviolobilin Provides the Foundation for the Blue/Green Photocycles in DXCF-Cyanobacteriochromes Burgie, E. Sethe; Walker, Joseph M.; Phillips, George N. Structure, Vol. 21, Issue 1 https://doi.org/10.1016/j.str.2012.11.001	journal	January 2013
Crystal Structure of Deinococcus Phytochrome in the Photoactivated State Reveals a Cascade of Structural Rearrangements during Photoconversion Burgie, E. Sethe; Zhang, Junrui; Vierstra, Richard D. Structure, Vol. 24, Issue 3 https://doi.org/10.1016/j.str.2016.01.001	journal	March 2016
Identification of Cyanobacteriochromes Detecting Far-Red Light Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, J. Clark Biochemistry, Vol. 55, Issue 28 https://doi.org/10.1021/acs.biochem.6b00299	journal	July 2016
Cyanobacteriochrome Photoreceptors Lacking the Canonical Cys Residue Fushimi, Keiji; Rockwell, Nathan C.; Enomoto, Gen Biochemistry, Vol. 55, Issue 50 https://doi.org/10.1021/acs.biochem.6b00940	journal	December 2016
Light-Regulated Synthesis of Cyclic-di-GMP by a Bidomain Construct of the Cyanobacteriochrome Tlr0924 (SesA) without Stable Dimerization Blain-Hartung, Matthew; Rockwell, Nathan C.; Lagarias, J. Clark Biochemistry, Vol. 56, Issue 46 https://doi.org/10.1021/acs.biochem.7b00734	journal	November 2017
Identification of the Deprotonated Pyrrole Nitrogen of the Bilin-Based Photoreceptor by Raman Spectroscopy with an Advanced Computational Analysis Osoegawa, Shinsuke; Miyoshi, Risako; Watanabe, Kouhei The Journal of Physical Chemistry B, Vol. 123, Issue 15 https://doi.org/10.1021/acs.jpcb.9b00965	journal	March 2019
Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli Ramakrishnan, Prabha; Tabor, Jeffrey J. ACS Synthetic Biology, Vol. 5, Issue 7 https://doi.org/10.1021/acssynbio.6b00068	journal	April 2016
Multiple-Site Diversification of Regulatory Sequences Enables Interspecies Operability of Genetic Devices Hueso-Gil, Angeles; Nyerges, Ákos; Pál, Csaba ACS Synthetic Biology, Vol. 9, Issue 1 https://doi.org/10.1021/acssynbio.9b00375	journal	December 2019
Photoisomerization by Hula-Twist: A Fundamental Supramolecular Photochemical Reaction Liu, Robert S. H. Accounts of Chemical Research, Vol. 34, Issue 7 https://doi.org/10.1021/ar000165c	journal	July 2001
Continuous Fluorescence Assay of Phytochrome Assembly in Vitro Li, Liming; Murphy, John T.; Lagarias, J. Clark Biochemistry, Vol. 34, Issue 24 https://doi.org/10.1021/bi00024a017	journal	June 1995
Light-Induced Proton Release and Proton Uptake Reactions in the Cyanobacterial Phytochrome Cph1 ^† van Thor, Jasper J.; Borucki, Berthold; Crielaard, Wim Biochemistry, Vol. 40, Issue 38 https://doi.org/10.1021/bi002651d	journal	September 2001
The Cyanobacteriochrome, TePixJ, Isomerizes Its Own Chromophore by Converting Phycocyanobilin to Phycoviolobilin Ishizuka, Takami; Kamiya, Ayumi; Suzuki, Hiroyuki Biochemistry, Vol. 50, Issue 6 https://doi.org/10.1021/bi101626t	journal	February 2011
Phycoviolobilin Formation and Spectral Tuning in the DXCF Cyanobacteriochrome Subfamily Rockwell, Nathan C.; Martin, Shelley S.; Gulevich, Alexander G. Biochemistry, Vol. 51, Issue 7 https://doi.org/10.1021/bi201783j	journal	February 2012
Mechanistic Insight into the Photosensory Versatility of DXCF Cyanobacteriochromes Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, J. Clark Biochemistry, Vol. 51, Issue 17 https://doi.org/10.1021/bi300171s	journal	April 2012
Red/Green Cyanobacteriochromes: Sensors of Color and Power Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, J. Clark Biochemistry, Vol. 51, Issue 48 https://doi.org/10.1021/bi3013565	journal	November 2012
Photoconversion Mechanism of the Second GAF Domain of Cyanobacteriochrome AnPixJ and the Cofactor Structure of Its Green-Absorbing State Velazquez Escobar, Francisco; Utesch, Tillmann; Narikawa, Rei Biochemistry, Vol. 52, Issue 29 https://doi.org/10.1021/bi400506a	journal	July 2013
A New Type of Dual-Cys Cyanobacteriochrome GAF Domain Found in Cyanobacterium Acaryochloris marina , Which Has an Unusual Red/Blue Reversible Photoconversion Cycle Narikawa, Rei; Enomoto, Gen Biochemistry, Vol. 53, Issue 31 https://doi.org/10.1021/bi500376b	journal	July 2014
A light-sensing knot revealed by the structure of the chromophore-binding domain of phytochrome Wagner, Jeremiah R.; Brunzelle, Joseph S.; Forest, Katrina T. Nature, Vol. 438, Issue 7066 https://doi.org/10.1038/nature04118	journal	November 2005
Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome Yang, Xiaojing; Ren, Zhong; Kuk, Jane Nature, Vol. 479, Issue 7373 https://doi.org/10.1038/nature10506	journal	October 2011
Structure of phycobilisome from the red alga Griffithsia pacifica Zhang, Jun; Ma, Jianfei; Liu, Desheng Nature, Vol. 551, Issue 7678 https://doi.org/10.1038/nature24278	journal	October 2017
Bright and stable near-infrared fluorescent protein for in vivo imaging Filonov, Grigory S.; Piatkevich, Kiryl D.; Ting, Li-Min Nature Biotechnology, Vol. 29, Issue 8 https://doi.org/10.1038/nbt.1918	journal	July 2011
An infrared fluorescent protein for deeper imaging Lecoq, Jérôme; Schnitzer, Mark J. Nature Biotechnology, Vol. 29, Issue 8 https://doi.org/10.1038/nbt.1941	journal	August 2011
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing Oliinyk, Olena S.; Shemetov, Anton A.; Pletnev, Sergei Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-018-08050-8	journal	January 2019
Diverse light responses of cyanobacteria mediated by phytochrome superfamily photoreceptors Wiltbank, Lisa B.; Kehoe, David M. Nature Reviews Microbiology, Vol. 17, Issue 1 https://doi.org/10.1038/s41579-018-0110-4	journal	November 2018
Photoconversion changes bilin chromophore conjugation and protein secondary structure in the violet/orange cyanobacteriochrome NpF2163g3 Lim, Sunghyuk; Rockwell, Nathan C.; Martin, Shelley S. Photochem. Photobiol. Sci., Vol. 13, Issue 6 https://doi.org/10.1039/C3PP50442E	journal	January 2014
Identification of DXCF cyanobacteriochrome lineages with predictable photocycles Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, J. Clark Photochemical & Photobiological Sciences, Vol. 14, Issue 5 https://doi.org/10.1039/C4PP00486H	journal	January 2015
Crystal-on-crystal chips for in situ serial diffraction at room temperature Ren, Zhong; Ayhan, Medine; Bandara, Sepalika Lab on a Chip, Vol. 18, Issue 15 https://doi.org/10.1039/C8LC00489G	journal	January 2018
Cyanobacteriochromes: a new superfamily of tetrapyrrole-binding photoreceptors in cyanobacteria Ikeuchi, Masahiko; Ishizuka, Takami Photochemical & Photobiological Sciences, Vol. 7, Issue 10 https://doi.org/10.1039/b802660m	journal	January 2008
Engineering of bacterial phytochromes for near-infrared imaging, sensing, and light-control in mammals Piatkevich, Kiryl D.; Subach, Fedor V.; Verkhusha, Vladislav V. Chemical Society Reviews, Vol. 42, Issue 8 https://doi.org/10.1039/c3cs35458j	journal	January 2013
Orange fluorescent proteins constructed from cyanobacteriochromes chromophorylated with phycoerythrobilin Sun, Ya-Fang; Xu, Jin-Guo; Tang, Kun Photochemical & Photobiological Sciences, Vol. 13, Issue 5 https://doi.org/10.1039/c3pp50411e	journal	January 2014
Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IX , a key enzyme in the biosynthesis of phycocyanobilin Hagiwara, Y.; Sugishima, M.; Takahashi, Y. Proceedings of the National Academy of Sciences, Vol. 103, Issue 1 https://doi.org/10.1073/pnas.0507266103	journal	December 2005
The structure of a complete phytochrome sensory module in the Pr ground state Essen, L. -O.; Mailliet, J.; Hughes, J. Proceedings of the National Academy of Sciences, Vol. 105, Issue 38 https://doi.org/10.1073/pnas.0806477105	journal	September 2008
Crystal structure of Pseudomonas aeruginosa bacteriophytochrome: Photoconversion and signal transduction Yang, X.; Kuk, J.; Moffat, K. Proceedings of the National Academy of Sciences, Vol. 105, Issue 38 https://doi.org/10.1073/pnas.0806718105	journal	September 2008
Distinct classes of red/far-red photochemistry within the phytochrome superfamily Rockwell, N. C.; Shang, L.; Martin, S. S. Proceedings of the National Academy of Sciences, Vol. 106, Issue 15 https://doi.org/10.1073/pnas.0902370106	journal	April 2009
Two ground state isoforms and a chromophore D-ring photoflip triggering extensive intramolecular changes in a canonical phytochrome Song, C.; Psakis, G.; Lang, C. Proceedings of the National Academy of Sciences, Vol. 108, Issue 10 https://doi.org/10.1073/pnas.1013377108	journal	February 2011
Diverse two-cysteine photocycles in phytochromes and cyanobacteriochromes Rockwell, N. C.; Martin, S. S.; Feoktistova, K. Proceedings of the National Academy of Sciences, Vol. 108, Issue 29 https://doi.org/10.1073/pnas.1107844108	journal	June 2011
Structures of cyanobacteriochromes from phototaxis regulators AnPixJ and TePixJ reveal general and specific photoconversion mechanism Narikawa, R.; Ishizuka, T.; Muraki, N. Proceedings of the National Academy of Sciences, Vol. 110, Issue 3 https://doi.org/10.1073/pnas.1212098110	journal	December 2012
Green/red cyanobacteriochromes regulate complementary chromatic acclimation via a protochromic photocycle Hirose, Y.; Rockwell, N. C.; Nishiyama, K. Proceedings of the National Academy of Sciences, Vol. 110, Issue 13 https://doi.org/10.1073/pnas.1302909110	journal	March 2013
Three cyanobacteriochromes work together to form a light color-sensitive input system for c-di-GMP signaling of cell aggregation Enomoto, Gen; Narikawa, Rei Proceedings of the National Academy of Sciences, Vol. 112, Issue 26 https://doi.org/10.1073/pnas.1504228112	journal	June 2015
Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4 Lim, Sunghyuk; Yu, Qinhong; Gottlieb, Sean M. Proceedings of the National Academy of Sciences, Vol. 115, Issue 17 https://doi.org/10.1073/pnas.1720682115	journal	April 2018
Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin Fushimi, Keiji; Miyazaki, Takatsugu; Kuwasaki, Yuto Proceedings of the National Academy of Sciences, Vol. 116, Issue 17 https://doi.org/10.1073/pnas.1818836116	journal	April 2019
Structural elements regulating the photochromicity in a cyanobacteriochrome Xu, Xiuling; Höppner, Astrid; Wiebeler, Christian Proceedings of the National Academy of Sciences, Vol. 117, Issue 5 https://doi.org/10.1073/pnas.1910208117	journal	January 2020
Structural basis of molecular logic OR in a dual-sensor histidine kinase Shin, Heewhan; Ren, Zhong; Zeng, Xiaoli Proceedings of the National Academy of Sciences, Vol. 116, Issue 40 https://doi.org/10.1073/pnas.1910855116	journal	September 2019
Genetic engineering of phytochrome biosynthesis in bacteria Gambetta, G. A.; Lagarias, J. C. Proceedings of the National Academy of Sciences, Vol. 98, Issue 19 https://doi.org/10.1073/pnas.191375198	journal	September 2001
Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE Nagae, Takayuki; Unno, Masashi; Koizumi, Taiki Proceedings of the National Academy of Sciences, Vol. 118, Issue 20 https://doi.org/10.1073/pnas.2024583118	journal	May 2021
Unusual Spectral Properties of Bacteriophytochrome Agp2 Result from a Deprotonation of the Chromophore in the Red-absorbing Form Pr Zienicke, Benjamin; Molina, Isabel; Glenz, René Journal of Biological Chemistry, Vol. 288, Issue 44 https://doi.org/10.1074/jbc.M113.479535	journal	September 2013
Structure and Mechanism of the Phycobiliprotein Lyase CpcT Zhou, Wei; Ding, Wen-Long; Zeng, Xiao-Li Journal of Biological Chemistry, Vol. 289, Issue 39 https://doi.org/10.1074/jbc.M114.586743	journal	July 2014
Highly Conserved Residues Asp-197 and His-250 in Agp1 Phytochrome Control the Proton Affinity of the Chromophore and Pfr Formation von Stetten, David; Seibeck, Sven; Michael, Norbert Journal of Biological Chemistry, Vol. 282, Issue 3 https://doi.org/10.1074/jbc.M608878200	journal	November 2006
Cyanobacteriochrome-based photoswitchable adenylyl cyclases (cPACs) for broad spectrum light regulation of cAMP levels in cells Blain-Hartung, Matthew; Rockwell, Nathan C.; Moreno, Marcus V. Journal of Biological Chemistry, Vol. 293, Issue 22 https://doi.org/10.1074/jbc.RA118.002258	journal	April 2018
Phytochrome functions in Arabidopsis development Franklin, K. A.; Quail, P. H. Journal of Experimental Botany, Vol. 61, Issue 1 https://doi.org/10.1093/jxb/erp304	journal	October 2009
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability Katoh, K.; Standley, D. M. Molecular Biology and Evolution, Vol. 30, Issue 4 https://doi.org/10.1093/molbev/mst010	journal	January 2013
Accounting for Solvent Accessibility and Secondary Structure in Protein Phylogenetics Is Clearly Beneficial Le, Si Quang; Gascuel, Olivier Systematic Biology, Vol. 59, Issue 3 https://doi.org/10.1093/sysbio/syq002	journal	March 2010
The structure of allophycocyanin B from Synechocystis PCC 6803 reveals the structural basis for the extreme redshift of the terminal emitter in phycobilisomes Peng, Pan-Pan; Dong, Liang-Liang; Sun, Ya-Fang Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 10 https://doi.org/10.1107/S1399004714015776	journal	September 2014
An automated platform for in situ serial crystallography at room temperature Ren, Zhong; Wang, Cong; Shin, Heewhan IUCrJ, Vol. 7, Issue 6 https://doi.org/10.1107/S2052252520011288	journal	September 2020
There and Back Again: Loss and Reacquisition of Two-Cys Photocycles in Cyanobacteriochromes Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, John Clark Photochemistry and Photobiology, Vol. 93, Issue 3 https://doi.org/10.1111/php.12708	journal	May 2017
Distinctive Properties of Dark Reversion Kinetics between Two Red/Green-Type Cyanobacteriochromes and their Application in the Photoregulation of cAMP Synthesis Fushimi, Keiji; Enomoto, Gen; Ikeuchi, Masahiko Photochemistry and Photobiology, Vol. 93, Issue 3 https://doi.org/10.1111/php.12732	journal	May 2017
The Expanded Red/Green Cyanobacteriochrome Lineage: An Evolutionary Hot Spot Fushimi, Keiji; Ikeuchi, Masahiko; Narikawa, Rei Photochemistry and Photobiology, Vol. 93, Issue 3 https://doi.org/10.1111/php.12764	journal	May 2017
UV‐Vis Spectroscopy Reveals a Correlation Between Y263 and BV Protonation States in Bacteriophytochromes Rumfeldt, Jessica A.; Takala, Heikki; Liukkonen, Alli Photochemistry and Photobiology, Vol. 95, Issue 4 https://doi.org/10.1111/php.13095	journal	March 2019
Long-range allosteric signaling in red light–regulated diguanylyl cyclases Gourinchas, Geoffrey; Etzl, Stefan; Göbl, Christoph Science Advances, Vol. 3, Issue 3 https://doi.org/10.1126/sciadv.1602498	journal	March 2017
Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome Shu, X.; Royant, A.; Lin, M. Z. Science, Vol. 324, Issue 5928 https://doi.org/10.1126/science.1168683	journal	May 2009
Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light Gan, F.; Zhang, S.; Rockwell, N. C. Science, Vol. 345, Issue 6202 https://doi.org/10.1126/science.1256963	journal	August 2014
Photoreceptor Signaling Networks in Plant Responses to Shade Casal, Jorge J. Annual Review of Plant Biology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev-arplant-050312-120221	journal	April 2013
Phytochrome Structure and Signaling Mechanisms Rockwell, Nathan C.; Su, Yi-Shin; Lagarias, J. Clark Annual Review of Plant Biology, Vol. 57, Issue 1 https://doi.org/10.1146/annurev.arplant.56.032604.144208	journal	June 2006
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues Chudakov, Dmitriy M.; Matz, Mikhail V.; Lukyanov, Sergey Physiological Reviews, Vol. 90, Issue 3 https://doi.org/10.1152/physrev.00038.2009	journal	July 2010
Crystal structure of a far-red cyanobacteriochrome photoreceptor at room temperature Yang, X.; Ren, Z.; Bandara, S. Worldwide Protein Data Bank https://doi.org/10.2210/pdb6uv8/pdb	text	January 2020
Crystal structure of far-red-light absorbing cyanobacteriochrome at 100K Yang, X.; Ren, Z.; Bandara, S. Worldwide Protein Data Bank https://doi.org/10.2210/pdb6uvb/pdb	text	January 2020
RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP) Zhao, Chi; Gan, Fei; Shen, Gaozhong Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01303	journal	November 2015
Photoconversion and Fluorescence Properties of a Red/Green-Type Cyanobacteriochrome AM1_C0023g2 That Binds Not Only Phycocyanobilin But Also Biliverdin Fushimi, Keiji; Nakajima, Takahiro; Aono, Yuki Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00588	journal	April 2016

Similar Records

A far-red cyanobacteriochrome lineage specific for verdins

Journal Article · Mon Oct 26 00:00:00 UTC 2020 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1690274

Cyanobacteriochromes: A Rainbow of Photoreceptors

Journal Article · Fri Jun 07 00:00:00 UTC 2024 · Annual Review of Microbiology · OSTI ID:2342095

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bilin-based photoreceptor
far-red-light sensing
linear tetrapyrrole tautomers
optogenetics
phycocyanobilin
serial crystallography

Crystal structure of a far-red–sensing cyanobacteriochrome reveals an atypical bilin conformation and spectral tuning mechanism

Citation Formats

References (77)

Similar Records

Related Subjects