Transmission of the atypical/Nor98 scrapie agent to Suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes

Cassmann, Eric D.; Mammadova, Najiba; Moore, S. Jo; Benestad, Sylvie; Greenlee, Justin J.

doi:10.1371/journal.pone.0246503

Transmission of the atypical/Nor98 scrapie agent to Suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes

Journal Article · Thu Feb 11 00:00:00 EST 2021 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0246503· OSTI ID:1905052

^[1]; Mammadova, Najiba ^[2]; Moore, S. Jo ^[2]; Benestad, Sylvie ^[3]; ^[2]

US Dept. of Agriculture (USDA)., Ames, IA (United States); OSTI
US Dept. of Agriculture (USDA)., Ames, IA (United States)
Norwegian Veterinary Institute, Oslo (Norway)

Scrapie is a transmissible spongiform encephalopathy that occurs in sheep. Atypical/Nor98 scrapie occurs in sheep that tend to be resistant to classical scrapie and it is thought to occur spontaneously. The purpose of this study was to test the transmission of the Atypical/Nor98 scrapie agent in three genotypes of Suffolk sheep and characterize the distribution of misfolded prion protein (PrP^Sc). Ten sheep were intracranially inoculated with brain homogenate from a sheep with Atypical/Nor98 scrapie. All sheep with the ARQ/ARQ and ARQ/ARR genotypes developed Atypical/Nor98 scrapie confirmed by immunohistochemistry, and one sheep with the VRQ/ARQ genotype had detectable PrP^Sc consistent with Atypical/Nor98 scrapie at the experimental endpoint of 8 years. Sheep with mild early accumulations of PrP^Sc in the cerebellum had concomitant retinal PrP^Sc. Accordingly, large amounts of retinal PrP^Sc were identified in clinically affected sheep and sheep with dense accumulations of PrP^Sc in the cerebellum.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1905052

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 2 Vol. 16; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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