Microbiomes of commercially-available pine nuts and sesame seeds

Fay, Megan; Salazar, Joelle K.; Ramachandran, Padmini; Stewart, Diana; Cardinale, Massimiliano

doi:10.1371/journal.pone.0252605

Title: Microbiomes of commercially-available pine nuts and sesame seeds

Journal Article · Mon Jun 21 00:00:00 EDT 2021 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0252605· OSTI ID:1904934

Fay, Megan ^[1];

^[1]; Ramachandran, Padmini ^[2]; Stewart, Diana ^[1]; Cardinale, Massimiliano ^[3]

U.S. Food and Drug Administration (FDA), Bedford Park, IL (United States)
U.S. Food and Drug Administration (FDA), College Park, MD (United States)
Univ. of Salento (Italy)

Metagenomic analysis of food is becoming more routine and can provide important information pertaining to the shelf life potential and the safety of these products. However, less information is available on the microbiomes associated with low water activity foods. Pine nuts and sesame seeds, and food products which contain these ingredients, have been associated with recalls due to contamination with bacterial foodborne pathogens. The objective of this study was to identify the microbial community of pine nuts and sesame seeds using targeted 16S rRNA sequencing technology. Ten different brands of each seed type were assessed, and core microbiomes were determined. A total of 21 and 16 unique taxa with proportional abundances >1% in at least one brand were identified in the pine nuts and sesame seeds, respectively. Members of the core pine nut microbiome included the genera Alishewanella, Aminivibrio, Mycoplasma, Streptococcus, and unassigned OTUs in the families of Desulfobacteraceae and Xanthomonadaceae. For sesame seeds, the core microbiome included Aminivibrio, Chryseolina, Okibacterium, and unassigned OTUs in the family Flavobacteriaceae. The microbiomes of these seeds revealed that these products are dominated by environmental bacterial genera commonly isolated from soil, water, and plants; bacterial genera containing species known as commensal organisms were also identified. Understanding these microbiomes can aid in the risk assessment of these products by identifying food spoilage potential and community members which may co-enrich with foodborne bacterial pathogens.

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Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); U.S. Food and Drug Administration (FDA)

Grant/Contract Number:: SC0014664

OSTI ID:: 1904934

Journal Information:: PLoS ONE, Vol. 16, Issue 6; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
microbiome
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food
bacillus
streptococcus
agricultural soil science
bacterial pathogens
seeds

Title: Microbiomes of commercially-available pine nuts and sesame seeds

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