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Title: The Tonoplast-Localized Sucrose Transporter in Populus (PtaSUT4) Regulates Whole-Plant Water Relations, Responses to Water Stress, and Photosynthesis

Journal Article · · PLoS ONE
 [1];  [1];  [1];  [1]
  1. University of Georgia, Athens, GA (United States)
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The Populus sucrose (Suc) transporter 4 (PtaSUT4), like its orthologs in other plant taxa, is tonoplast localized and thought to mediate Suc export from the vacuole into the cytosol. In source leaves of Populus, SUT4 is the predominantly expressed gene family member, with transcript levels several times higher than those of plasma membrane SUTs. A hypothesis is advanced that SUT4-mediated tonoplast sucrose fluxes contribute to the regulation of osmotic gradients between cellular compartments, with the potential to mediate both sink provisioning and drought tolerance in Populus. Here, we describe the effects of PtaSUT4-RNA interference (RNAi) on sucrose levels and raffinose family oligosaccharides (RFO) induction, photosynthesis, and water uptake, retention and loss during acute and chronic drought stresses. Under normal waterreplete growing conditions, SUT4-RNAi plants had generally higher shoot water contents than wild-type plants. In response to soil drying during a short-term, acute drought, RNAi plants exhibited reduced rates of water uptake and delayed wilting relative to wild-type plants. SUT4-RNAi plants had larger leaf areas and lower photosynthesis rates than wild-type plants under well-watered, but not under chronic water-limiting conditions. Moreover, the magnitude of shoot water content, height growth, and photosynthesis responses to contrasting soil moisture regimes was greater in RNAi than wild-type plants. The concentrations of stress-responsive RFOs increased in wild-type plants but were unaffected in SUT4-RNAi plants under chronically dry conditions. We discuss a model in which the subcellular compartmentalization of sucrose mediated by PtaSUT4 is regulated in response to both sink demand and plant water status in Populus.

Research Organization:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
SC0005140
OSTI ID:
1904518
Journal Information:
PLoS ONE, Vol. 7, Issue 8; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Characterization, localization, and seasonal changes of the sucrose transporter FeSUT1 in the phloem ofFraxinus excelsior journal May 2015
Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus journal August 2015
Salicylic acid activates poplar defense against the biotrophic rust fungus Melampsora larici‐populina via increased biosynthesis of catechin and proanthocyanidins journal August 2018
An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought journal October 2018
Genome-wide identification of MST, SUT and SWEET family sugar transporters in root parasitic angiosperms and analysis of their expression during host parasitism journal May 2019
Effects of External Potassium (K) Supply on Drought Tolerances of Two Contrasting Winter Wheat Cultivars journal July 2013
Molecular Evolution and Stress and Phytohormone Responsiveness of SUT Genes in Gossypium hirsutum journal October 2018
Source-to-sink transport of sugar and regulation by environmental factors journal January 2013
Bayesian phylogeny of sucrose transporters: ancient origins, differential expansion and convergent evolution in monocots and dicots journal November 2014
The Plasma Membrane-Localized Sucrose Transporter IbSWEET10 Contributes to the Resistance of Sweet Potato to Fusarium oxysporum journal February 2017
Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro journal June 2017

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59 BASIC BIOLOGICAL SCIENCES
leaves
water resources
RNA interface
genetically modified plants
photosynthesis
poplars
light
plant vacuoles