AnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactor

Pérez‐González, Ana; Jimenez‐Vicente, Emilio; Salinero‐Lanzarote, Alvaro; Harris, Derek F.; Seefeldt, Lance C.; Dean, Dennis R.

doi:10.1111/mmi.14890

AnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactor

Journal Article · Sat Feb 26 23:00:00 EST 2022 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.14890· OSTI ID:1904478

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Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA (United States); OSTI
Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA (United States)
University Politecnica de Madrid (Spain)
Utah State University, Logan, UT (United States)

Azotobacter vinelandii produces three genetically distinct, but structurally and mechanistically similar nitrogenase isozymes designated as Mo-dependent, V-dependent, or Fe-only based on the heterometal contained within their associated active site cofactors. These catalytic cofactors, which provide the site for N₂ binding and reduction, are, respectively, designated as FeMo-cofactor, FeV-cofactor, and FeFe-cofactor. Fe-only nitrogenase is a poor catalyst for N₂ fixation, when compared to the Modependent and V-dependent nitrogenases and is only produced when neither Mo nor V is available. Under conditions favoring the production of Fe-only nitrogenase a gene product designated AnfO preserves the fidelity of Fe-only nitrogenase by preventing the misincorporation of FeV-cofactor, which results in the accumulation of a hybrid enzyme that cannot reduce N₂. These results are interpreted to indicate that AnfO controls the fidelity of Fe-only nitrogenase maturation during the physiological transition from conditions that favor V-dependent nitrogenase utilization to Fe-only nitrogenase utilization to support diazotrophic growth.

View Accepted Manuscript (DOE)

Research Organization:: Utah State University, Logan, UT (United States); Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Bill and Melinda Gates Foundation

Grant/Contract Number:: SC0010687

OSTI ID:: 1904478

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 5 Vol. 117; ISSN 0950-382X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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AnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactor

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