n-Butanol production by Rhodopseudomonas palustris TIE-1

Bai, Wei; Ranaivoarisoa, Tahina Onina; Singh, Rajesh; Rengasamy, Karthikeyan; Bose, Arpita

doi:10.1038/s42003-021-02781-z

n-Butanol production by Rhodopseudomonas palustris TIE-1

Journal Article · Wed Nov 03 04:00:00 EDT 2021 · Communications Biology

DOI:https://doi.org/10.1038/s42003-021-02781-z· OSTI ID:1904440

^[1]; Ranaivoarisoa, Tahina Onina ^[2]; Singh, Rajesh ^[2]; Rengasamy, Karthikeyan ^[2]; ^[2]

Washington Univ., St. Louis, MO (United States); Washington Univ., St. Louis, MO (United States)
Washington Univ., St. Louis, MO (United States)

Anthropogenic carbon dioxide (CO₂) release in the atmosphere from fossil fuel combustion has inspired scientists to study CO₂ to biofuel conversion. Oxygenic phototrophs such as cyanobacteria have been used to produce biofuels using CO₂. However, oxygen generation during oxygenic photosynthesis adversely affects biofuel production efficiency. To produce n-butanol (biofuel) from CO₂, here we introduce an n-butanol biosynthesis pathway into an anoxygenic (non-oxygen evolving) photoautotroph, Rhodopseudomonas palustris TIE-1 (TIE-1). Using different carbon, nitrogen, and electron sources, we achieve n-butanol production in wild-type TIE-1 and mutants lacking electron-consuming (nitrogen-fixing) or acetyl-CoA-consuming (polyhydroxybutyrate and glycogen synthesis) pathways. The mutant lacking the nitrogen-fixing pathway produce the highest n-butanol. Coupled with novel hybrid bioelectrochemical platforms, this mutant produces n-butanol using CO₂, solar panel-generated electricity, and light with high electrical energy conversion efficiency. Overall, this approach showcases TIE-1 as an attractive microbial chassis for carbon-neutral n-butanol bioproduction using sustainable, renewable, and abundant resources.

View Accepted Manuscript (DOE)

Research Organization:: Washington Univ., St. Louis, MO (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); US Army Research Office (ARO); Gordon and Betty Moore Foundation; USDOD DEPSCoR; David and Lucile Packard Foundation

Grant/Contract Number:: SC0014613; AC52-07NA27344

OSTI ID:: 1904440

Report Number(s):: LLNL-JRNL-812309; PII: 2781

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 4; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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