The effects of xylo-oligosaccharides on regulating growth performance, nutrient utilization, gene expression of tight junctions, nutrient transporters, and cecal short chain fatty acids profile in Eimeria-challenged broiler chickens

Lin, Yang; Teng, Po-Yun; Olukosi, Oluyinka A.

doi:10.1016/j.psj.2022.102125

Title: The effects of xylo-oligosaccharides on regulating growth performance, nutrient utilization, gene expression of tight junctions, nutrient transporters, and cecal short chain fatty acids profile in Eimeria-challenged broiler chickens

Journal Article · Tue Aug 09 00:00:00 EDT 2022 · Poultry Science

DOI:https://doi.org/10.1016/j.psj.2022.102125· OSTI ID:1904432

^[1]; Teng, Po-Yun ^[1];

^[1]

Univ. of Georgia, Athens, GA (United States)

A 21-d experiment was conducted to investigate the effects of xylo-oligosaccharides (XOS) on growth performance, nutrient utilization, gene expression of tight junctions, nutrient transporters, and cecal short chain fatty acids (SCFA) profile of broiler chickens challenged with mixed Eimeria spp. Two hundred fifty-two zero-day-old chicks were allocated to 6 treatments in a 3 £ 2 factorial arrangement (corn-soybean meal diets supplemented with 0, 0.5, or 1.0 g/kg XOS; with or without Eimeria challenge). Challenged groups were inoculated with a solution containing E. maxima, E. acervulina, and E. tenella oocysts on d 15. During the infection period (d 15 to d 21), there was a significant (P < 0.05) Eimeria £ XOS interaction for weight gain (WG). XOS significantly (P < 0.05) increased WG in the unchallenged birds but not in the challenged treatments. There was no significant Eimeria £ XOS interaction for N and minerals utilization responses. XOS supplementation at 0.5 g/kg tended to alleviate Eimeria-induced depression in apparent ileal digestibility of DM (P = 0.052). Challenged birds had lower (P < 0.01) AME, AMEn, and total retention of N, Ca, and P. Eimeria upregulated (P < 0.01) gene expression of tight junction proteins claudin-1, junctional adhesion molecule-2, and glucose transporter GLUT1; but downregulated (P < 0.01) the peptide transporter PepT1, amino acid transporters rBAT, CAT2, y+LAT2, and zinc transporter ZnT1. XOS alleviated (P < 0.05) Eimeria-induced claudin-1 upregulation. Eimeria decreased (P < 0.05) cecal saccharolytic SCFA acetate, butyrate, and total SCFA, but increased (P < 0.05) branched chain fatty acids isobutyrate and isovalerate. The supplementation of XOS tended to decrease the concentration of isobutyrate (P = 0.08) and isovalerate (P = 0.062). In conclusion, 0.5 g/kg XOS supplementation alleviated depression in growth performance and nutrient utilization from the Eimeria challenge. In addition, supplemental XOS reversed the gene expression changes of claudin-1, also showed the potentials of alleviating the negative cecal fermentation pattern induced by Eimeria infection.

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Research Organization:: Univ. of Georgia, Athens, GA (United States); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDA

Grant/Contract Number:: SC0015662; 58-6040-8-034

OSTI ID:: 1904432

Alternate ID(s):: OSTI ID: 1962846

Journal Information:: Poultry Science, Vol. 101, Issue 11; ISSN 0032-5791

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (51)

Prebiotics can restrict Salmonella populations in poultry: a review Abd El-Hack, Mohamed E.; El-Saadony, Mohamed T.; Shafi, Manal E. Animal Biotechnology, Vol. 33, Issue 7 https://doi.org/10.1080/10495398.2021.1883637	journal	February 2021
Overview of Prebiotics and Probiotics: Focus on Performance, Gut Health and Immunity – A Review Adhikari, Pratima Acharya; Kim, Woo Kyun Annals of Animal Science, Vol. 17, Issue 4 https://doi.org/10.1515/aoas-2016-0092	journal	October 2017
Recent Advances in Biology and Immunobiology of Eimeria Species and in Diagnosis and Control of Infection with These Coccidian Parasites of Poultry Allen, P. C.; Fetterer, R. H. Clinical Microbiology Reviews, Vol. 15, Issue 1 https://doi.org/10.1128/CMR.15.1.58-65.2002	journal	January 2002
Age-Related Differences in the Luminal and Mucosa-Associated Gut Microbiome of Broiler Chickens and Shifts Associated with Campylobacter jejuni Infection Awad, Wageha A.; Mann, Evelyne; Dzieciol, Monika Frontiers in Cellular and Infection Microbiology, Vol. 6 https://doi.org/10.3389/fcimb.2016.00154	journal	November 2016
Effects of Purified Lignin and Mannan Oligosaccharides on Intestinal Integrity and Microbial Populations in the Ceca and Litter of Broiler Chickens Baurhoo, B.; Phillip, L.; Ruiz-Feria, C. A. Poultry Science, Vol. 86, Issue 6 https://doi.org/10.1093/ps/86.6.1070	journal	June 2007
Arabinoxylan-oligosaccharides kick-start arabinoxylan digestion in the aging broiler Bautil, A.; Verspreet, J.; Buyse, J. Poultry Science, Vol. 99, Issue 5 https://doi.org/10.1016/j.psj.2019.12.041	journal	May 2020
The evolution and application of enzymes in the animal feed industry: the role of data interpretation Bedford, Michael R. British Poultry Science, Vol. 59, Issue 5 https://doi.org/10.1080/00071668.2018.1484074	journal	July 2018
The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens Biswas, Avishek; Dev, Kapil; Tyagi, Pramod K. Animal Bioscience, Vol. 35, Issue 1 https://doi.org/10.5713/ab.20.0749	journal	January 2022
Spotlight on avian pathology:Eimeriaand the disease coccidiosis Blake, Damer P.; Marugan-Hernandez, Virginia; Tomley, Fiona M. Avian Pathology, Vol. 50, Issue 3 https://doi.org/10.1080/03079457.2021.1912288	journal	April 2021
The similarity of the effect of carbohydrase or prebiotic supplementation in broilers aged 21 days, fed mixed cereal diets and challenged with coccidiosis infection Craig, Allison D.; Khattak, Farina; Hastie, Peter PLOS ONE, Vol. 15, Issue 2 https://doi.org/10.1371/journal.pone.0229281	journal	February 2020
Xylanase and xylo- oligosaccharide prebiotic improve the growth performance and concentration of potentially prebiotic oligosaccharides in the ileum of broiler chickens Craig, A. D.; Khattak, F.; Hastie, P. British Poultry Science, Vol. 61, Issue 1 https://doi.org/10.1080/00071668.2019.1673318	journal	October 2019
Poultry coccidiosis: recent advancements in control measures and vaccine development Dalloul, Rami A.; Lillehoj, Hyun S. Expert Review of Vaccines, Vol. 5, Issue 1 https://doi.org/10.1586/14760584.5.1.143	journal	February 2006
Effect of dietary xylooligosaccharides on intestinal characteristics, gut microbiota, cecal short-chain fatty acids, and plasma immune parameters of laying hens Ding, X. M.; Li, D. D.; Bai, S. P. Poultry Science, Vol. 97, Issue 3 https://doi.org/10.3382/ps/pex372	journal	March 2018
Xylooligosaccharide Modulates Gut Microbiota and Alleviates Colonic Inflammation Caused by High Fat Diet Induced Obesity Fei, Yanquan; Wang, Yan; Pang, Yilin Frontiers in Physiology, Vol. 10 https://doi.org/10.3389/fphys.2019.01601	journal	January 2020
Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics Gibson, Glenn R.; Hutkins, Robert; Sanders, Mary Ellen Nature Reviews Gastroenterology & Hepatology, Vol. 14, Issue 8 https://doi.org/10.1038/nrgastro.2017.75	journal	June 2017
Dietary modulation of the human colonic microbiota: updating the concept of prebiotics Gibson, Glenn R.; Probert, Hollie M.; Loo, Jan Van Nutrition Research Reviews, Vol. 17, Issue 2 https://doi.org/10.1079/NRR200479	journal	December 2004
Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics Gibson, Glenn R.; Roberfroid, Marcel B. The Journal of Nutrition, Vol. 125, Issue 6 https://doi.org/10.1093/jn/125.6.1401	journal	June 1995
Effect of dietary fiber levels on performance, gizzard development, intestinal morphology, and nutrient utilization in meat ducks from 1 to 21 days of age Han, H. Y.; Zhang, K. Y.; Ding, X. M. Poultry Science, Vol. 96, Issue 12 https://doi.org/10.3382/ps/pex268	journal	December 2017
Dietary Xylooligosaccharide Downregulates IFN-γ and the Low-Grade Inflammatory Cytokine IL-1β Systemically in Mice Hansen, Camilla H. F.; Frøkiær, Hanne; Christensen, Annette G. The Journal of Nutrition, Vol. 143, Issue 4 https://doi.org/10.3945/jn.112.172361	journal	February 2013
Anticoccidial drugs: Lesion scoring techniques in battery and floor-pen experiments with chickens Johnson, Joyce; Reid, W. Malcolm Experimental Parasitology, Vol. 28, Issue 1 https://doi.org/10.1016/0014-4894(70)90063-9	journal	August 1970
Xylo-oligosaccharide (XOS) in combination with inulin modulates both the intestinal environment and immune status in healthy subjects, while XOS alone only shows prebiotic properties Lecerf, Jean-Michel; Dépeint, Flore; Clerc, Elise British Journal of Nutrition, Vol. 108, Issue 10 https://doi.org/10.1017/S0007114511007252	journal	January 2012
Effects of dietary xylooligosaccharides on the performance, egg quality, nutrient digestibility and plasma parameters of laying hens Li, D. D.; Ding, X. M.; Zhang, K. Y. Animal Feed Science and Technology, Vol. 225 https://doi.org/10.1016/j.anifeedsci.2016.12.010	journal	March 2017
Exogenous Enzymes Influenced Eimeria-Induced Changes in Cecal Fermentation Profile and Gene Expression of Nutrient Transporters in Broiler Chickens Lin, Yang; Olukosi, Oluyinka A. Animals, Vol. 11, Issue 9 https://doi.org/10.3390/ani11092698	journal	September 2021
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Livak, Kenneth J.; Schmittgen, Thomas D. Methods, Vol. 25, Issue 4 https://doi.org/10.1006/meth.2001.1262	journal	December 2001
Effects of Xylo-Oligosaccharides on Broiler Chicken Performance and Microbiota De Maesschalck, C.; Eeckhaut, V.; Maertens, L. Applied and Environmental Microbiology, Vol. 81, Issue 17 https://doi.org/10.1128/AEM.01616-15	journal	September 2015
Prebiotics Manning, Thea Scantlebury; Gibson, Glenn R. Best Practice & Research Clinical Gastroenterology, Vol. 18, Issue 2 https://doi.org/10.1016/j.bpg.2003.10.008	journal	April 2004
The mRNA expression of amino acid and sugar transporters, aminopeptidase, as well as the di- and tri-peptide transporter PepT1 in the intestines of Eimeria infected broiler chickens Miska, K. B.; Fetterer, R. H. Poultry Science, Vol. 96, Issue 2 https://doi.org/10.3382/ps/pew303	journal	February 2017
Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens Mookiah, Saminathan; Sieo, Chin Chin; Ramasamy, Kalavathy Journal of the Science of Food and Agriculture, Vol. 94, Issue 2 https://doi.org/10.1002/jsfa.6365	journal	September 2013
Effect of arabinoxylo-oligosaccharides and arabinoxylans on net energy and nutrient utilization in broilers Morgan, Natalie K.; Keerqin, Chake; Wallace, Andrew Animal Nutrition, Vol. 5, Issue 1 https://doi.org/10.1016/j.aninu.2018.05.001	journal	March 2019
Advances in the manufacture, purification and applications of xylo-oligosaccharides as food additives and nutraceuticals Moure, Andrés; Gullón, Patricia; Domínguez, Herminia Process Biochemistry, Vol. 41, Issue 9 https://doi.org/10.1016/j.procbio.2006.05.011	journal	September 2006
Non-digestible oligosaccharides: A review Mussatto, Solange I.; Mancilha, Ismael M. Carbohydrate Polymers, Vol. 68, Issue 3 https://doi.org/10.1016/j.carbpol.2006.12.011	journal	April 2007
The Role of Microbial Amino Acid Metabolism in Host Metabolism Neis, Evelien; Dejong, Cornelis; Rensen, Sander Nutrients, Vol. 7, Issue 4 https://doi.org/10.3390/nu7042930	journal	April 2015
16S rRNA amplicon sequencing characterization of caecal microbiome composition of broilers and free-range slow-growing chickens throughout their productive lifespan Ocejo, Medelin; Oporto, Beatriz; Hurtado, Ana Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-39323-x	journal	February 2019
Increase in the Tight Junction Protein Claudin-1 in Intestinal Inflammation Poritz, Lisa S.; Harris, Leonard R.; Kelly, Ashley A. Digestive Diseases and Sciences, Vol. 56, Issue 10 https://doi.org/10.1007/s10620-011-1688-9	journal	July 2011
Prebiotic effects: metabolic and health benefits Roberfroid, Marcel; Gibson, Glenn R.; Hoyles, Lesley British Journal of Nutrition, Vol. 104, Issue S2 https://doi.org/10.1017/S0007114510003363	journal	August 2010
Effects of prebiotics on immune system and cytokine expression Shokryazdan, Parisa; Faseleh Jahromi, Mohammad; Navidshad, Bahman Medical Microbiology and Immunology, Vol. 206, Issue 1 https://doi.org/10.1007/s00430-016-0481-y	journal	October 2016
Determination of titanium dioxide added as an inert marker in chicken digestibility studies Short, F. J.; Gorton, P.; Wiseman, J. Animal Feed Science and Technology, Vol. 59, Issue 4 https://doi.org/10.1016/0377-8401(95)00916-7	journal	June 1996
Xylooligosaccharides chemical stability after high-intensity ultrasound processing of prebiotic orange juice Silva, Eric Keven; Arruda, Henrique S.; Pastore, Glaucia M. Ultrasonics Sonochemistry, Vol. 63 https://doi.org/10.1016/j.ultsonch.2019.104942	journal	May 2020
Effects of supplemental xylanase and xylooligosaccharides on production performance and gut health variables of broiler chickens Singh, Amit K.; Mishra, Birendra; Bedford, Michael R. Journal of Animal Science and Biotechnology, Vol. 12, Issue 1 https://doi.org/10.1186/s40104-021-00617-8	journal	September 2021
Expression of digestive enzymes and nutrient transporters in Eimeria acervulina-challenged layers and broilers Su, S.; Miska, K. B.; Fetterer, R. H. Poultry Science, Vol. 93, Issue 5 https://doi.org/10.3382/ps.2013-03807	journal	May 2014
Expression of digestive enzymes and nutrient transporters in Eimeria-challenged broilers Su, S.; Miska, K. B.; Fetterer, R. H. Experimental Parasitology, Vol. 150 https://doi.org/10.1016/j.exppara.2015.01.003	journal	March 2015
Effectiveness of dietary xylo-oligosaccharides for broilers fed a conventional corn-soybean meal diet Suo, Hai-qing; Lu, Lin; Xu, Guo-hui Journal of Integrative Agriculture, Vol. 14, Issue 10 https://doi.org/10.1016/S2095-3119(15)61101-7	journal	October 2015
Impacts of increasing challenge with Eimeria maxima on the growth performance and gene expression of biomarkers associated with intestinal integrity and nutrient transporters Teng, Po-Yun; Choi, Janghan; Tompkins, Yuguo Veterinary Research, Vol. 52, Issue 1 https://doi.org/10.1186/s13567-021-00949-3	journal	June 2021
Effects of low-crude protein diets supplemented with arginine, glutamine, threonine, and methionine on regulating nutrient absorption, intestinal health, and growth performance of Eimeria-infected chickens Teng, Po-Yun; Choi, Janghan; Yadav, Sudhir Poultry Science, Vol. 100, Issue 11 https://doi.org/10.1016/j.psj.2021.101427	journal	November 2021
Graded Eimeria challenge linearly regulated growth performance, dynamic change of gastrointestinal permeability, apparent ileal digestibility, intestinal morphology, and tight junctions of broiler chickens Teng, Po-Yun; Yadav, Sudhir; Castro, Fernanda Lima de Souza Poultry Science, Vol. 99, Issue 9 https://doi.org/10.1016/j.psj.2020.04.031	journal	September 2020
Effects of coccidial vaccination and dietary antimicrobial alternatives on the growth performance, internal organ development, and intestinal morphology of Eimeria-challenged male broilers Wang, Xi; Peebles, E. David; Kiess, Aaron S. Poultry Science, Vol. 98, Issue 5 https://doi.org/10.3382/ps/pey552	journal	May 2019
Prebiotics, Bone and Mineral Metabolism Whisner, Corrie M.; Castillo, Luisa F. Calcified Tissue International, Vol. 102, Issue 4 https://doi.org/10.1007/s00223-017-0339-3	journal	October 2017
Xylooligosaccharide supplementation alters gut bacteria in both healthy and prediabetic adults: a pilot study Yang, Jieping; Summanen, Paula H.; Henning, Susanne M. Frontiers in Physiology, Vol. 6 https://doi.org/10.3389/fphys.2015.00216	journal	August 2015
Dietary xylo-oligosaccharide improves intestinal functions in weaned piglets Yin, Jie; Li, Fengna; Kong, Xiangfeng Food & Function, Vol. 10, Issue 5 https://doi.org/10.1039/C8FO02485E	journal	January 2019
Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens Yuan, Lin; Li, Wanli; Huo, Qianqian PeerJ, Vol. 6 https://doi.org/10.7717/peerj.4435	journal	March 2018
Dietary supplemental xylooligosaccharide modulates nutrient digestibility, intestinal morphology, and gut microbiota in laying hens Zhou, Jianmin; Wu, Shugeng; Qi, Guanghai Animal Nutrition, Vol. 7, Issue 1 https://doi.org/10.1016/j.aninu.2020.05.010	journal	March 2021

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Title: The effects of xylo-oligosaccharides on regulating growth performance, nutrient utilization, gene expression of tight junctions, nutrient transporters, and cecal short chain fatty acids profile in Eimeria-challenged broiler chickens

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