Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)

Tiedge, Kira; Destremps, Janessa; Solano-Sanchez, Janet; Arce-Rodriguez, Magda Lisette; Zerbe, Philipp

doi:10.1186/s13007-022-00903-0

Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)

Journal Article · Mon May 30 00:00:00 EDT 2022 · Plant Methods

DOI:https://doi.org/10.1186/s13007-022-00903-0· OSTI ID:1870352

; Destremps, Janessa; Solano-Sanchez, Janet; ;

Abstract Background

Although the genome for the allotetraploid bioenergy crop switchgrass ( Panicum virgatum ) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimization. Virus-induced gene silencing (VIGS) is a versatile technique for transient genetic studies. Here we report the implementation of foxtail mosaic virus (FoMV)-mediated gene silencing in switchgrass in above- and below-ground tissues and at different developmental stages.

Results

The study demonstrated that leaf rub-inoculation is a suitable method for systemic gene silencing in switchgrass. For all three visual marker genes, Magnesium chelatase subunit D ( ChlD ) and I ( ChlI ) as well as phytoene desaturase ( PDS ), phenotypic changes were observed in leaves, albeit at different intensities. Gene silencing efficiency was verified by RT-PCR for all tested genes. Notably, systemic gene silencing was also observed in roots, although silencing efficiency was stronger in leaves (~ 63–94%) as compared to roots (~ 48–78%). Plants at a later developmental stage were moderately less amenable to VIGS than younger plants, but also less perturbed by the viral infection.

Conclusions

Using FoMV-mediated VIGS could be achieved in switchgrass leaves and roots, providing an alternative approach for studying gene functions and physiological traits in this important bioenergy crop.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0019178

OSTI ID:: 1870352

Alternate ID(s):: OSTI ID: 1904359

Journal Information:: Plant Methods, Journal Name: Plant Methods Journal Issue: 1 Vol. 18; ISSN 1746-4811

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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