Brachypodium sylvaticum, a Model for Perennial Grasses: Transformation and Inbred Line Development

Steinwand, Michael A.; Young, Hugh A.; Bragg, Jennifer N.; Tobias, Christian M.; Vogel, John P.

doi:10.1371/journal.pone.0075180

Title: Brachypodium sylvaticum, a Model for Perennial Grasses: Transformation and Inbred Line Development

Journal Article · Fri Sep 20 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0075180· OSTI ID:1904008

Steinwand, Michael A. ^[1]; Young, Hugh A. ^[2]; Bragg, Jennifer N. ^[3]; Tobias, Christian M. ^[1]; Vogel, John P. ^[1]

US Department of Agriculture (USDA), Albany, CA (United States). Agricultural Research Service
US Department of Agriculture (USDA), Albany, CA (United States). Agricultural Research Service; University of California, Berkeley, CA (United States)
US Department of Agriculture (USDA), Albany, CA (United States). Agricultural Research Service; University of California, Davis, CA (United States)

Perennial species offer significant advantages as crops including reduced soil erosion, lower energy inputs after the first year, deeper root systems that access more soil moisture, and decreased fertilizer inputs due to the remobilization of nutrients at the end of the growing season. These advantages are particularly relevant for emerging biomass crops and it is projected that perennial grasses will be among the most important dedicated biomass crops. The advantages offered by perennial crops could also prove favorable for incorporation into annual grain crops like wheat, rice, sorghum and barley, especially under the dryer and more variable climate conditions projected for many grain-producing regions. Thus, it would be useful to have a perennial model system to test biotechnological approaches to crop improvement and for fundamental research. The perennial grass Brachypodium sylvaticum is a candidate for such a model because it is diploid, has a small genome, is self-fertile, has a modest stature, and short generation time. Its close relationship to the annual model Brachypodium distachyon will facilitate comparative studies and allow researchers to leverage the resources developed for B. distachyon. Here we report on the development of two keystone resources that are essential for a model plant: high-efficiency transformation and inbred lines. Using Agrobacterium tumefaciens-mediated transformation we achieved an average transformation efficiency of 67%. We also surveyed the genetic diversity of 19 accessions from the National Plant Germplasm System using SSR markers and created 15 inbred lines.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: University of California, Berkeley, CA (United States); US Department of Agriculture (USDA), Albany, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA

OSTI ID:: 1904008

Journal Information:: PLoS ONE, Vol. 8, Issue 9; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (41)

Production of transgenic creeping bentgrass Agrostis stolonifera var. palustris plants by Agrobacterium tumefaciens-mediated transformation using hygromycin selection Han, Ning; Chen, Dong; Bian, Hong-Wu Plant Cell, Tissue and Organ Culture, Vol. 81, Issue 2 https://doi.org/10.1007/s11240-004-4042-5	journal	May 2005
Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat Griffiths, Simon; Sharp, Rebecca; Foote, Tracie N. Nature, Vol. 439, Issue 7077 https://doi.org/10.1038/nature04434	journal	February 2006
A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium Lazo, Gerard R.; Stein, Pascal A.; Ludwig, Robert A. Bio/Technology, Vol. 9, Issue 10 https://doi.org/10.1038/nbt1091-963	journal	October 1991
Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection Bragg, Jennifer N.; Wu, Jiajie; Gordon, Sean P. PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0041916	journal	September 2012
The Pseudo-Response Regulator Ppd-H1 Provides Adaptation to Photoperiod in Barley Turner, A. Science, Vol. 310, Issue 5750 https://doi.org/10.1126/science.1117619	journal	November 2005
Delayed leaf senescence induces extreme drought tolerance in a flowering plant Rivero, R. M.; Kojima, M.; Gepstein, A. Proceedings of the National Academy of Sciences, Vol. 104, Issue 49 https://doi.org/10.1073/pnas.0709453104	journal	November 2007
Missing domesticated plant forms: can artificial selection fill the gap? Van Tassel, David L.; DeHaan, Lee R.; Cox, Thomas S. Evolutionary Applications, Vol. 3, Issue 5-6 https://doi.org/10.1111/j.1752-4571.2010.00132.x	journal	May 2010
Prospects for Developing Perennial Grain Crops Cox, Thomas S.; Glover, Jerry D.; Van Tassel, David L. BioScience, Vol. 56, Issue 8 https://doi.org/10.1641/0006-3568(2006)56[649:PFDPGC]2.0.CO;2	journal	January 2006
Perennial cereal crops: An initial evaluation of wheat derivatives Hayes, R. C.; Newell, M. T.; DeHaan, L. R. Field Crops Research, Vol. 133 https://doi.org/10.1016/j.fcr.2012.03.014	journal	July 2012
Transformation of recalcitrant turfgrass cultivars through improvement of tissue culture and selection regime Cao, M. X.; Huang, J. Q.; He, Y. L. Plant Cell, Tissue and Organ Culture, Vol. 85, Issue 3 https://doi.org/10.1007/s11240-006-9081-7	journal	May 2006
Annual Dry Matter Production and Partitioning Over the First 5 Years of a Bed System of Crispin/M.27 Apple Trees at Four Spacings Palmer, J. W. The Journal of Applied Ecology, Vol. 25, Issue 2 https://doi.org/10.2307/2403845	journal	August 1988
Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance Kim, Soo Jung; Lee, Jee-Yeon; Kim, Yong-Min Journal of Plant Biology, Vol. 50, Issue 5 https://doi.org/10.1007/BF03030712	journal	October 2007
Brachypodium as a Model for the Grasses: Today and the Future Brkljacic, Jelena; Grotewold, Erich; Scholl, Randy Plant Physiology, Vol. 157, Issue 1 https://doi.org/10.1104/pp.111.179531	journal	July 2011
More Productive Than Maize in the Midwest: How Does Miscanthus Do It? Dohleman, Frank G.; Long, Stephen P. Plant Physiology, Vol. 150, Issue 4 https://doi.org/10.1104/pp.109.139162	journal	June 2009
Ribosomal DNA variation and its phylogenetic implication in the genusBrachypodium (Poaceae) Shi, Ying; Draper, John; Stace, Clive Plant Systematics and Evolution, Vol. 188, Issue 3-4 https://doi.org/10.1007/BF00937726	journal	January 1994
Cytokinin-mediated source/sink modifications improve drought tolerance and increase grain yield in rice under water-stress: Cytokinin-mediated drought tolerance in rice Peleg, Zvi; Reguera, Maria; Tumimbang, Ellen Plant Biotechnology Journal, Vol. 9, Issue 7 https://doi.org/10.1111/j.1467-7652.2010.00584.x	journal	February 2011
Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon Vogel, John P.; Tuna, Metin; Budak, Hikmet BMC Plant Biology, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2229-9-88	journal	January 2009
T-DNA insertional mutagenesis for functional genomics in rice Jeon, Jong-Seong; Lee, Sichul; Jung, Ki-Hong The Plant Journal, Vol. 22, Issue 6 https://doi.org/10.1046/j.1365-313x.2000.00767.x	journal	June 2000
Increased Food and Ecosystem Security via Perennial Grains Glover, J. D.; Reganold, J. P.; Bell, L. W. Science, Vol. 328, Issue 5986 https://doi.org/10.1126/science.1188761	journal	June 2010
Agrobacterium tumefaciens -mediated creeping bentgrass ( Agrostis stolonifera L.) transformation using phosphinothricin selection results in a high frequency of single-copy transgene integration Luo, H.; Hu, Q.; Nelson, K. Plant Cell Reports, Vol. 22, Issue 9 https://doi.org/10.1007/s00299-003-0734-2	journal	April 2004
Assaying chimeric genes in plants: The GUS gene fusion system Jefferson, Richard A. Plant Molecular Biology Reporter, Vol. 5, Issue 4 https://doi.org/10.1007/BF02667740	journal	December 1987
Comparison of orthologous loci from small grass genomes Brachypodium and rice: implications for wheat genomics and grass genome annotation Bossolini, Eligio; Wicker, Thomas; Knobel, Philip A. The Plant Journal, Vol. 49, Issue 4 https://doi.org/10.1111/j.1365-313X.2006.02991.x	journal	January 2007
Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34 /Yr18: a disease resistance locus effective against multiple pathogens in wheat Spielmeyer, W.; Singh, R. P.; McFadden, H. Theoretical and Applied Genetics, Vol. 116, Issue 4 https://doi.org/10.1007/s00122-007-0684-0	journal	December 2007
Genome sequencing and analysis of the model grass Brachypodium distachyon Nature, Vol. 463, Issue 7282, p. 763-768 https://doi.org/10.1038/nature08747	journal	February 2010
EST sequencing and phylogenetic analysis of the model grass Brachypodium distachyon Vogel, John P.; Gu, Yong Qiang; Twigg, Paul Theoretical and Applied Genetics, Vol. 113, Issue 2, p. 186-195 https://doi.org/10.1007/s00122-006-0285-3	journal	May 2006
Stimulatory Effects of Casein Hydrolysate and Proline in in vitro Callus Induction and Plant Regeneration from Five Deepwater Rice (Oryza sativa L.) ., L. Khaleda; ., M. Al-Forkan Biotechnology(Faisalabad), Vol. 5, Issue 3 https://doi.org/10.3923/biotech.2006.379.384	journal	June 2006
Rapid and efficient production of transgenic bermudagrass and creeping bentgrass bypassing the callus formation phase Wang, Zeng-Yu; Ge, Yaxin Functional Plant Biology, Vol. 32, Issue 9 https://doi.org/10.1071/FP05083	journal	January 2005
Perennial wheat: a review of environmental and agronomic prospects for development in Australia Bell, Lindsay W.; Wade, Len J.; Ewing, Mike A. Crop and Pasture Science, Vol. 61, Issue 9 https://doi.org/10.1071/CP10064	journal	January 2010
Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon Vogel, John P.; Garvin, David F.; Leong, Oymon M. Plant Cell, Tissue and Organ Culture, Vol. 84, Issue 2 https://doi.org/10.1007/s11240-005-9023-9	journal	January 2006
Physiological perspectives of changes over time in maize yield dependency on nitrogen uptake and associated nitrogen efficiencies: A review Ciampitti, Ignacio A.; Vyn, Tony J. Field Crops Research, Vol. 133 https://doi.org/10.1016/j.fcr.2012.03.008	journal	July 2012
Isolation and characterization of nine microsatellite markers forBrachypodium sylvaticum(Huds.) Beauv., a recently invasive grass species in Oregon Ramakrishnan, Alisa P.; Rosenthal, David M.; Musial, Trieste Molecular Ecology Resources, Vol. 8, Issue 6 https://doi.org/10.1111/j.1755-0998.2008.02174.x	journal	November 2008
-Mediated Genetic Transformation of Switchgrass Somleva, M. N.; Tomaszewski, Z.; Conger, B. V. Crop Science, Vol. 42, Issue 6 https://doi.org/10.2135/cropsci2002.2080	journal	January 2002
Progress in breeding perennial grains Cox, T. S.; Van Tassel, D. L.; Cox, C. M. Crop and Pasture Science, Vol. 61, Issue 7 https://doi.org/10.1071/CP09201	journal	January 2010
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3 Vogel, John; Hill, Theresa Plant Cell Reports, Vol. 27, Issue 3 https://doi.org/10.1007/s00299-007-0472-y	journal	November 2007
Evolutionary History of the Grasses Kellogg, Elizabeth A. Plant Physiology, Vol. 125, Issue 3 https://doi.org/10.1104/pp.125.3.1198	journal	March 2001
Cellulosic Biofuels Carroll, Andrew; Somerville, Chris Annual Review of Plant Biology, Vol. 60, Issue 1 https://doi.org/10.1146/annurev.arplant.043008.092125	journal	June 2009
Breeding Perennial Grain Crops Cox, T. S.; Bender, M.; Picone, C. Critical Reviews in Plant Sciences, Vol. 21, Issue 2 https://doi.org/10.1080/0735-260291044188	journal	March 2002
T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum Feldmann, Kenneth A. The Plant Journal, Vol. 1, Issue 1 https://doi.org/10.1111/j.1365-313X.1991.00071.x	journal	July 1991
A high throughput Agrobacterium tumefaciens-mediated transformation method for functional genomics of perennial ryegrass (Lolium perenne L.) Bajaj, Shivendra; Ran, Yidong; Phillips, Jonathan Plant Cell Reports, Vol. 25, Issue 7 https://doi.org/10.1007/s00299-005-0099-9	journal	March 2006
Breeding relationships in the genus Brachypodium (Poaceae: Pooideae) Khan, Mir Ajab; Stace, Clive A. Nordic Journal of Botany, Vol. 19, Issue 3 https://doi.org/10.1111/j.1756-1051.1999.tb01108.x	journal	July 1999
Karyotype variation is indicative of subgenomic and ecotypic differentiation in switchgrass Young, Hugh A.; Sarath, Gautam; Tobias, Christian M. BMC Plant Biology, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2229-12-117	journal	January 2012

Cited By (4)

Progress of cereal transformation technology mediated by Agrobacterium tumefaciens Hiei, Yukoh; Ishida, Yuji; Komari, Toshihiko Frontiers in Plant Science, Vol. 5 https://doi.org/10.3389/fpls.2014.00628	journal	November 2014
Use of Agrobacterium rhizogenes Strain 18r12v and Paromomycin Selection for Transformation of Brachypodium distachyon and Brachypodium sylvaticum Collier, Ray; Bragg, Jennifer; Hernandez, Bryan T. Frontiers in Plant Science, Vol. 7 https://doi.org/10.3389/fpls.2016.00716	journal	May 2016
Comparatively Barcoded Chromosomes of Brachypodium Perennials Tell the Story of Their Karyotype Structure and Evolution Lusinska, Joanna; Betekhtin, Alexander; Lopez-Alvarez, Diana International Journal of Molecular Sciences, Vol. 20, Issue 22 https://doi.org/10.3390/ijms20225557	journal	November 2019
Genetic structure and diversity of the selfing model grassBrachypodium stacei(Poaceae) in Western Mediterranean: out of the Iberian Peninsula and into the islands Shiposha, Valeriia; Catalán, Pilar; Olonova, Marina PeerJ, Vol. 4 https://doi.org/10.7717/peerj.2407	journal	September 2016

Similar Records

Agrobacterium ‐Mediated Transformation of Brachypodium distachyon

Journal Article · Tue Mar 12 00:00:00 EDT 2019 · Current Protocols in Plant Biology · OSTI ID:1904008

Chen, Fengjuan; Liu, Qi; P. Vogel, John; +1 more

The reference genome and abiotic stress responses of the model perennial grass Brachypodium sylvaticum

Journal Article · Thu Oct 26 00:00:00 EDT 2023 · G3 · OSTI ID:1904008

Lei, Li; Gordon, Sean P.; Liu, Lifeng; +17 more

Using the Model Perennial Grass Brachypodium sylvaticum to Engineer Resistance to Multiple Abiotic Stresses

Conference · Fri Mar 20 00:00:00 EDT 2015 · OSTI ID:1904008

Gordon, Sean; Reguera, Maria; Sade, Nir; +5 more

Related Subjects

54 ENVIRONMENTAL SCIENCES
genetically modified plants
grasses
crops
flowering plants
seeds
inbred strains
agrobacteria
phylogenetic analysis

Title: Brachypodium sylvaticum, a Model for Perennial Grasses: Transformation and Inbred Line Development

Citation Formats

References (41)

Cited By (4)

Similar Records

Related Subjects